Isolation of the Tomato
            <i>Cf-9</i>
            Gene for Resistance to
            <i>Cladosporium fulvum</i>
            by Transposon Tagging

Jones, David A.; Thomas, Colwyn M.; Hammond‐Kosack, K. E.; Balint‐Kurti, Peter; Jones, Jonathan D. G.

doi:10.1126/science.7973631

Cited by 821 publications

(517 citation statements)

References 33 publications

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“…The use of such transposable elements as insertional mutagens in heterologous plant species has been investigated, and it has been established that the maize transposable elements AciDs are transpositionally active when introduced into other plant species. The application of the maize Activator (Ac) and Dissociation (Ds) elements as insertional mutagens in heterologous host species has resulted in genes being cloned from tobacco (Whitham et aL, 1994), tomato (Jones et aL, 1994), Arabidopsis thaliana (Bancroft et aL, 1993) and N. ptumbaginifolia (Marinet aL, 1996). In parallel with the application of this technique, much research has been directed into characterizing the mechanism by which the transposition process occurs.…”

Section: Introductionmentioning

confidence: 99%

Alternative processing of the maize Ac transcript in Arabidopsis

et al. 1997

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SummaryThe successful application of the maize transposable element system AciDs as a genome mutagen in heterologous plant species has recently proved the versatility and power of this technique in plant molecular biology. However, the frequency of Ac/Ds transposition is considerably lower in Arabidopsis thaliana than in most other dicot plant species that have been studied. Since previous research has established that transcripts derived from monocot genes can be alternatively processed in dicot plants, we have investigated both the efficiency of intron splicing and poiyadenylation of the maize Ac transposase pre-mRNA in Arabidopsis thaliana, Nicotiana tabacum, Nicotiana plumbaginifolia and Zea mays. In this paper, we demonstrate that intron 4 is alternatively spliced within Arabidopsis, using cryptic 5' and 3' splice sites within the intron sequence, leading to a heterogeneous population of full length transposase transcript. Furthermore, analysis of transposase transcript polyadenylation revealed that at least four alternative poly(A) sites were utilized between introns 2 and 3, resulting in truncated transposase transcripts. Finally, by Northern blotting, we established that the truncated transposase transcript was the most abundant form of transposase message in Arabidopsis. In contrast to these findings, the alternative splicing and premature polyadenylation of Ac message in Arabidopsis was unparalleled in the other species examined. We suggest that the poor frequency of transposition of Ac in Arabidopsis may be in part due to the low quantity of correctly processed transposase transcript available in this species.

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Section: Introductionmentioning

confidence: 99%

Alternative processing of the maize Ac transcript in Arabidopsis

et al. 1997

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“…Transposition of AciDs to linked sites occurs in maize as well as several heterologous hosts including Arabidopsis Keller et al, 1993). This aspect of the behaviour of Ac/Ds was used to isolate genes by targeted transposon-tagging from maize (Dellaporta et al, 1988), tomato (Jones et al, 1994) and Arabidopsis (James et al, 1995). A prerequisite for this strategy is a copy of the transposon close to the gene of interest.…”

Section: Introductionmentioning

confidence: 99%

Ds elements on all five Arabidopsis chromosomes and assessment of their utility for transposon tagging

et al. 1997

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SummaryThe maize transposons Activator (Ac) and Dissociation (Ds) tend to transpose to sites close to their original position and can be efficiently used to transposon-tag genetically linked genes. To facilitate this approach, we describe the locations of seven T-DNAs carrying Ds elements, including at least one on each of the five chromosomes. For five of these T-DNAs, we have confirmed that the Ds element transposes preferentially to genetically linked sites. A large-scale transposon-tagging experiment was performed by activating Ds from eight chromosomal locations that included at least one on each of the five chromosomes. These experiments produced a total of 1132 F 3 families that were predicted to carry around 870 independent Ds insertions. In these populations, 33 independently isolated mutants that were visibly different from wild-type were identified. Twenty-nine of these mutants were studied genetically, and 14 were not tagged with Ds because the element could be separated from the mutation by recombination. The remaining 15 mutations were possibly tagged because the transposon and the mutation were not separated by recombination. These experiments provide tools for transposon-tagging on each chromosome, and indicate that approximately 50% of identified mutations are likely to be tagged, thereby enabling cloning of the affected genes.

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“…The discovery of the RXC3 gene encoding for Xanthomonas recognition/reaction extends the number of pathogen specificities located within this interval. Cloning and characResistance in Arabidopsis to X. co campestris 27 terization of RXC3 will allow comparison of a digenic resistance gene not only to other recently cloned resistance genes (Bent et al, 1994;Grant et al, 1995;Jones et al, 1994;Lawrence et aL, 1995;Mindrinos et aL, 1994;Song et al, 1995;Whitham et al, 1994), but also to other resistance genes located in the MRC-J interval (Lee et aL, 1996;McDowell et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

“…RPS2 and RPM1/RPS3, which confer resistance to Pseudomonas syringae pathovars, encode proteins with a leucine-rich repeat domain (Bent et al, 1994;Grant et al, 1995;Mindrinos et al, 1994). This feature is conserved in several other disease resistance genes, such as the Cfo9, N, L e and Xa21 genes cloned from other angiosperms, indicating a conservation in resistance mechanisms to bacterial, viral and fungal pathogens in flowering plants (Jones et al, 1994;Lawrence et al, 1995;Song et aL, 1995;Whitham et aL, 1994).…”

Section: Introductionmentioning

confidence: 99%

Use of Arabidopsis recombinant inbred lines reveals a monogenic and a novel digenic resistance mechanism to Xanthomonas campestris pv campestris

Buell

Somerville

1997

The Plant Journal

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SummaryInfiltration of the Arabidopsis thaliana accession Landsberg erecta (Ler) with Xanthomonas campestris pv campestris isolate 2D520 results in extensive necrosis and limited chlorosis within 5-6 days post-inoculation (d.p.i.}, which can lead to systemic necrosis within 23 d.p.i. In contrast, the accession Columbia (Col) remains asymptomatic after infiltration. Although both accessions support bacterial growth, 5-28-fold more bacteria are present in Let than in Col leaf tissue. Inheritance studies indicate that three independent, dominant or partially dominant, nuclear genes condition resistance to X. c. campestris 2D520. The major gene, termed RXC2, conditions monogenic resistance to X. c. campestris and was mapped to a 5.5 cM interval of chromosome V. Segregation data indicate that the locus RXC3 in conjunction with RXC4 confers digenic resistance to X. c. campestris. The combined action of RXC3 and RXC4 is correlated with a suppression of in planta bacterial levels and a suppression of symptoms relative to Ler. The RXC3 + RXC4-mediated resistance is novel in that although the Col allele of RXC4 contributes positively to resistance, it is the Ler and not the Col allele of RXC3 that contributes positively to resistance. RXC3 was mapped to the bottom arm of chromosome V in a 2.7 cM interval within the major recognition gene complex MRC-J, a cluster of genes involved in disease resistance. RXC4 was mapped to a 12 cM interval on chromosome II that also contains RXCl, a gene conferring tolerance to X. c. campestris.

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Isolation of the Tomato Cf-9 Gene for Resistance to Cladosporium fulvum by Transposon Tagging

Cited by 821 publications

References 33 publications

Alternative processing of the maize Ac transcript in Arabidopsis

Alternative processing of the maize Ac transcript in Arabidopsis

Ds elements on all five Arabidopsis chromosomes and assessment of their utility for transposon tagging

Use of Arabidopsis recombinant inbred lines reveals a monogenic and a novel digenic resistance mechanism to Xanthomonas campestris pv campestris

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