Isolation of T-Cell Tropic HTLV-III-Like Retrovirus from Macaques

Daniel, Daniel; Letvin, N L; King, Norval W.; Kannagi, Mari; Sehgal, Priya; Hunt, Ronald D.; Kanki, PJ; Essex, Max; Desrosiers, R. C.

doi:10.1126/science.3159089

Cited by 847 publications

(427 citation statements)

References 24 publications

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“…Of special interest was the identification of HIV-l-specific MAbs cross-reacting also with HIV-2, strains LAV-2ro d and SBL 6669 (Clavel et al, 1986;Albert et al, 1987), and with SIVma c (Daniel et al, 1985). Four different reaction patterns were observed with regard to reactivity in ELISA and immunoblotting using antigens derived from partially purified HTLV-IIIB, LAV-2, and SIVma~ (Table I).…”

Section: Mab Reactivitiesmentioning

confidence: 99%

Monoclonal Antibodies Directed against Human Immunodeficiency Virus (HIV) gag Proteins with Specificity for Conserved Epitopes in HIV-1, HIV-2 and Simian Immunodeficiency Virus

Niedrig

Rabanus

Stehr

et al. 1988

Journal of General Virology

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Section: Mab Reactivitiesmentioning

confidence: 99%

Monoclonal Antibodies Directed against Human Immunodeficiency Virus (HIV) gag Proteins with Specificity for Conserved Epitopes in HIV-1, HIV-2 and Simian Immunodeficiency Virus

Niedrig

Rabanus

Stehr

et al. 1988

Journal of General Virology

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“…In this study full-length molecular clones have been isolated from a virus pool known as the 32H reisolate of SIVmac, originating from a rhesus macaque infected with the same pool of SIVmac from which three other molecular clones, SIVmac251(pBK28), SIVmaclAll and SIVmac239 have previously been isolated (Daniel et al, 1985;Naidu et aI., 1988 ;Unger et al, 1992). The 32H molecular clones are being used to examine the ability of subunit and attenuated versions of the clones to stimulate protective responses in vaccinated macaques.…”

Section: Introductionmentioning

confidence: 99%

Molecular and biological characterization of simian immunodeficiency virus macaque strain 32H proviral clones containing nef size variants

Rud

Cranage²,

Yon

et al. 1994

Journal of General Virology

122

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The proviral genome of the 32H reisolate of simian immunodeficiency of macaques (SIVmac32H) has been cloned and sequenced. Including both long terminal repeats, it is 10277 base pairs in length and contains open reading frames for all known SIV genes (gag, pol, vif, vpx, ~pr, tat, rev, env and neJ). This is the first report of an infectious SIVmac molecular clone which contains no premature termination codons. Three molecular clones of SIVmac32H have been constructed differing in sequence only within their last 1.2 kb. Two of the molecular clones, SIVmac32H(pJS) and SIVmae32H (pC8), differ in the nef coding region by an in-frame deletion of four amino acids in pC8 and two conservative amino acid changes; other nucleotide changes in the 3' LTR were not associated with known functionally critical motifs. The third clone, SIVmac32H(pB1), contains the last 1.2 kb of the SIVmae251 clone pBK28. The biological properties of virus produced after electroporation of these clones into C8166 cells has been assessed by infection of rhesus and cynomolgus macaques, time to seroconversion and by induction of cytopathic effects upon co-cultivation of infected rhesus peripheral blood lymphocytes with C8166 cells. The viruses obtained from these clones have identical growth kinetics in vitro but differ in their ability to persist in macaques. Macaques infected with p J5 derived virus remain viraemic longer than macaques infected with pC8-derived virus. PCR analysis of circulating provirus indicates that the nefgene evolved over time in p J5 virusinfected macaques, whereas late in infection in pC8 virus-infected macaques the nefgene remained invariant in sequence. These results support the observation that a nefdeletion mutant of SIVmac239 lost its pathogenic potential and resulted in low-level viraemia when rhesus macaques were infected. Virus challenge pools for vaccine studies have been prepared for p J5 using both human and monkey cell substrates and these stocks have been titrated both in vitro and in vivo. Virus has also been prepared from pC8 and titrated in vitro. This virus pool is being assessed as an attenuated live-virus vaccine in macaques. Since only virus originating from the SIVmac239 molecular clone is known to cause AIDSlike symptoms in rhesus macaques consistently, the SIVmac32H molecular clones should tell us more about which viral sequence features are important for the pathogenesis of AIDS.

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“…Similar to HIV infection of humans, infection with SIV causes an AIDS-like disease in the majority of infected macaques (30). SIV and HIV are also similar in that both have tropism for CD4 ϩ T cells (31,32) and that structurally diverse HIV and SIV envelope glycoproteins use similar members of the chemokine receptor family as coreceptors (33,34). Furthermore, HLA-A and -B locus homologues are found in rhesus macaques (21), and both human and macaque (Macaca mulatta) MHC class I (Mamu) 3 molecules appear to bind gag and env epitopes from similar regions of both HIV and SIV (35).…”

mentioning

confidence: 99%

Conserved MHC Class I Peptide Binding Motif Between Humans and Rhesus Macaques

Dzuris¹,

Sidney²,

Appella

et al. 2000

The Journal of Immunology

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Since the onset of the HIV pandemic, the use of nonhuman primate models of infection has increasingly become important. An excellent model to study HIV infection and immunological responses, in particular cell-mediated immune responses, is SIV infection of rhesus macaques. CTL epitopes have been mapped using SIV-infected rhesus macaques, but, to date, a peptide binding motif has been described for only one rhesus class I MHC molecule, Mamu-A*01. Herein, we have established peptide-live cell binding assays for four rhesus MHC class I molecules: Mamu-A*11, -B*03, -B*04, and -B*17. Using such assays, peptide binding motifs have been established for all four of these rhesus MHC class I molecules. With respect to the nature and spacing of crucial anchor positions, the motifs defined for Mamu-B*04 and -B*17 present unique features not previously observed for other primate species. The motifs identified for Mamu-A*11 and -B*03 are very similar to the peptide binding motifs previously described for human HLA-B*44 and -B*27, respectively. Accordingly, naturally processed peptides derived from HLA-B*44 and HLA-B*27 specifically bind Mamu-A*11 and Mamu-B*03, respectively, indicating that conserved MHC class I binding capabilities exist between rhesus macaques and humans. The definition of four rhesus MHC class I-specific motifs expands our ability to accurately detect and quantitate immune responses to MHC class I-restricted epitopes in rhesus macaques and to rationally design peptide epitope-based model vaccine constructs destined for use in nonhuman primates.

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Isolation of T-Cell Tropic HTLV-III-Like Retrovirus from Macaques

Cited by 847 publications

References 24 publications

Monoclonal Antibodies Directed against Human Immunodeficiency Virus (HIV) gag Proteins with Specificity for Conserved Epitopes in HIV-1, HIV-2 and Simian Immunodeficiency Virus

Monoclonal Antibodies Directed against Human Immunodeficiency Virus (HIV) gag Proteins with Specificity for Conserved Epitopes in HIV-1, HIV-2 and Simian Immunodeficiency Virus

Molecular and biological characterization of simian immunodeficiency virus macaque strain 32H proviral clones containing nef size variants

Conserved MHC Class I Peptide Binding Motif Between Humans and Rhesus Macaques

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