Isolation of sodium chloride-tolerant plantlets of Kickxia ramosissima under in vitro conditions

Tissue Culture in the Improvement of Salt Tolerance in Plants

“…Selection in vitro also results in the isolation of cells that express enhanced salt tolerance after the stress is eliminated and the phenotype is stable through regeneration (Mathur et al 1980;Nabors et al 1980Nabors et al , 1982Tyagi et al 1981;Yano et al 1982;Bressan et al 1985Bressan et al , 1987Nabors and Dykes 1985;Bhaskaran et al 1986;Pua and Thorpe 1986;McCoy 1987b;Bouharmont and Dekeyser 1989;Hanning and Nabors 1989;Narayanan and Sree Rangasamy 1989;Vajrabhaya et al 1989;Freytag et al 1990;Jain et al 1990Jain et al , 1991Waskom et al 1990;Watad et al 1991b). Difficulties in isolating cells that possess a genetic capacity to tolerate high salt concentrations from those that are exhibiting an inherent capacity for adjustment into saline environments have been circumvented either by isolating cell clones or through establishment of selection protocols capable of identifying cells with a genetically based predisposition for salt tolerance (Dix and Street 1975;Nabors 1983Nabors , 1990Bressan et al 1985;van Swaaji et al 1986;Ochatt and Power 1988;Tal 1990).…”

A protein induced by NaCl in suspension cultures ofNicotiana tabacum accumulates in whole plant roots

“…However, suspension cultures provide an opportunity to (i) work with a relatively homogeneous population of cells and (ii) limit the number of responses to only those which operate at the cellular level. We should be able to extrapolate to the whole plant from data obtained with suspension cells because ~esults obtained using suspension cultures have demonstrated that (i) some of the phenotypic differences in salt tolerance which occur in whole plants are retained in cell cultures derived from these plants (1~5, 18) and (ii) that salt tolerant plants have been regenerated from cells adapted to NaC1 stress in tissue culture (13,14). Nonetheless, it is important that once responses in tissue culture have been identified, these be' correlated with responses which occur in the intact stressed plant.…”

NaCl-resistant variant cells isolated from sweet potato cell suspensions

“…Cell lines with increased tolerance to NaC1 have been isolated from Medicago sativa [3], Nicotiana sylvestris and Capsicum annuum [4], Citrus sinensis and C. aurantium [9], Nicotiana tabacum [12], Oryza sativa [15], Pennisetum americanum [16], and Datura innoxia [17]. Regeneration of NaCl-tolerant plants from tissue cultures also has been reported [10,13,17].…”