Isolation of single cells for protein therapeutics using microwell selection and Surface Plasmon Resonance imaging

Abali, Fekri; Stevens, Michiel; Tibbe, Arjan G.J.; Terstappen, Leon W.M.M.; Velde, P.N. van der; Schasfoort, Richardus B.M.

doi:10.1016/j.ab.2017.05.021

Cited by 23 publications

(22 citation statements)

References 11 publications

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“…To overcome this, some technology providers are developing solutions to punch out the cells from well-shaped filter pores (e.g. VyCAP filters [ 34 ]). Further examples for more widely used microfluidic devices are the Parsortix device [ 35 , 36 ], the ClearCell® FX1 [ 37 ] or the Vortex trapping system [ 38 , 39 ].…”

Section: Classes Of Blood-based Biomarkers In Liquid Biopsymentioning

confidence: 99%

ctDNA and CTCs in Liquid Biopsy – Current Status and Where We Need to Progress

Neumann

Bender

Krahn

et al. 2018

Computational and Structural Biotechnology Journal

176

145

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Section: Classes Of Blood-based Biomarkers In Liquid Biopsymentioning

confidence: 99%

ctDNA and CTCs in Liquid Biopsy – Current Status and Where We Need to Progress

Neumann

Bender

Krahn

et al. 2018

Computational and Structural Biotechnology Journal

176

145

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show abstract

“…The technology is well suited for the isolation of single cells from cell suspensions. Examples are the isolation of trophoblasts from maternal blood for non‐invasive‐prenatal testing (NIPT), isolation of single cells from cell suspensions obtained from for example the dissociated of Zebrafish embryos, organoids, or tissues or isolation of cells from cell lines and clonal expansion of single cells .…”

Section: Resultsmentioning

confidence: 99%

VyCAP's puncher technology for single cell identification, isolation, and analysis

et al. 2018

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Here we present the Puncher technology for the isolation of single cells. This technology combines a silicon chip with microwells, fluorescence imaging, and a punching method to isolate and transfer the single cells to standard reaction tubes. The technology is compatible with commercially available downstream workflows and instrumentation. Here we focus on the isolation of CTC but the Puncher technology can be applied to isolate single cells from liquid biopsies and more general from cell suspensions. It is especially suited for cell suspensions that contain: • Cells of interest at a frequency of 1 per 10,000 or less • A low total number of cells ranging from 1 to 100,000, that are present in a volume of 0.01 to 50 mL.The frequency of appearance of CTC in blood is in the order of the 1 per 10 6 leukocytes.To be able to isolate the single CTC with the Puncher technology, enrichment of the CTC by a 3 logs reduction of the leukocytes is required. Here we describe the use of Rosettesep and Parsortix as examples of pre-enrichment methods that are compatible with the Puncher technology and further downstream applications.

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“…The protocol for selecting single cells based on criteria as the quality and quantity of the secreted product is according to the procedure described by Abali et al [44]:…”

Section: Quantifying the Secretion Of Cellsmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters

et al. 2018

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SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.

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Isolation of single cells for protein therapeutics using microwell selection and Surface Plasmon Resonance imaging

Cited by 23 publications

References 11 publications

ctDNA and CTCs in Liquid Biopsy – Current Status and Where We Need to Progress

ctDNA and CTCs in Liquid Biopsy – Current Status and Where We Need to Progress

VyCAP's puncher technology for single cell identification, isolation, and analysis

Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters

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