Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters

Schasfoort, Richardus B.M.; Abali, Fikri; Stojanović, Ivan; Vidarsson, Gestur; Terstappen, Leon W.M.M.

doi:10.3390/bios8040102

Cited by 22 publications

(16 citation statements)

References 44 publications

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“…Indeed, in SPR imaging, signal results from amplitude modulation at a fixed angle, unlike GC-SPR where a phase angle scan is performed. Because they are based on intensity interrogation, SPR imaging sensors suffer from worse performance with respect to angle scanning systems ( [20] and for a complete review see [21]). In conclusion, our methodological approach results in good resolution, compact, inexpensive and very simple cell detection setup, and opens for further research strategies based on the integration of the sensitive grating into a microfluidic chamber that could improve cell biology studies.…”

Section: Discussionmentioning

confidence: 99%

Label-free, real-time on-chip sensing of living cancer cell via grating-coupled surface plasmon resonance

Borile

Rossi

Filippi

et al. 2019

Preprint

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The application of nanotechnologies to address biomedical questions is a key strategy for innovation in biomedical research. Among others, a key point consists in the availability of nanotechnologies for monitoring cellular processes in a real-time and label-free approach. Here, we focused on a grating-coupled Surface Plasmon Resonance (GC-SPR) sensor exploiting phase interrogation. This sensor can be integrated in a microfluidic chamber that ensures cell viability and avoids cell stress. We report the calibration of the sensor response as a function of cell number and its application to monitor cell adhesion kinetics as well as cell response to an external stimulus. Our results show that GC-SPR sensors can offer a valuable alternative to prism-coupled or imaging SPR devices, amenable for microfluidic implementation.

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Section: Discussionmentioning

confidence: 99%

Label-free, real-time on-chip sensing of living cancer cell via grating-coupled surface plasmon resonance

Borile

Rossi

Filippi

et al. 2019

Preprint

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“…With each well containing a single cell, and most of the cell's secreted species diffusing towards the SPR surface rather than the bulk, the ability of SPRi instruments to take measurements at multiple detection spots enables a comparison of the secretions of different cells [156]. For more details, the reader is referred to a recent review on the advances in the field of SPR cytometry [157].…”

Section: Spr Studies With Live Cellsmentioning

confidence: 99%

On the Use of Surface Plasmon Resonance-Based Biosensors for Advanced Bioprocess Monitoring

et al. 2021

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Biomanufacturers are being incited by regulatory agencies to transition from a quality by testing framework, where they extensively test their product after their production, to more of a quality by design or even quality by control framework. This requires powerful analytical tools and sensors enabling measurements of key process variables and/or product quality attributes during production, preferably in an online manner. As such, the demand for monitoring technologies is rapidly growing. In this context, we believe surface plasmon resonance (SPR)-based biosensors can play a role in enabling the development of improved bioprocess monitoring and control strategies. The SPR technique has been profusely used to probe the binding behavior of a solution species with a sensor surface-immobilized partner in an investigative context, but its ability to detect binding in real-time and without a label has been exploited for monitoring purposes and is promising for the near future. In this review, we examine applications of SPR that are or could be related to bioprocess monitoring in three spheres: biotherapeutics production monitoring, vaccine monitoring, and bacteria and contaminant detection. These applications mainly exploit SPR’s ability to measure solution species concentrations, but performing kinetic analyses is also possible and could prove useful for product quality assessments. We follow with a discussion on the limitations of SPR in a monitoring role and how recent advances in hardware and SPR response modeling could counter them. Mainly, throughput limitations can be addressed by multi-detection spot instruments, and nonspecific binding effects can be alleviated by new antifouling materials. A plethora of methods are available for cell growth and metabolism monitoring, but product monitoring is performed mainly a posteriori. SPR-based biosensors exhibit potential as product monitoring tools from early production to the end of downstream processing, paving the way for more efficient production control. However, more work needs to be done to facilitate or eliminate the need for sample preprocessing and to optimize the experimental protocols.

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“…This relatively large sensing depth allows SPR to monitor the changes occurred not only on surfaces, but also in the bulk. SPR has been applied in various studies for molecules with molecular weight between 1000 Da~500 kDa [47]. Liu et al [48] attached human ovarian carcinoma SKOV-3 cells on the ceiling of a flow cell chamber, and detected their vascular endothelial growth factor (VEGF) secretion by monitoring the SPR signal of the antibodiesimmobilized gold chip at the bottom of the flow cell.…”

Section: Plasmonic Sensorsmentioning

confidence: 99%

Advanced Biosensing towards Real-Time Imaging of Protein Secretion from Single Cells

Zhou¹,

Chen²,

Simonian³

2021

Biosensors - Current and Novel Strategies for Biosensing

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Protein secretion of cells plays a vital role in intercellular communication. The abnormality and dysfunction of cellular protein secretion are associated with various physiological disorders, such as malignant proliferation of cells, aberrant immune function, and bone marrow failure. The heterogeneity of protein secretion exists not only between varying populations of cells, but also in the same phenotype of cells. Therefore, characterization of protein secretion from single cell contributes not only to the understanding of intercellular communication in immune effector, carcinogenesis and metastasis, but also to the development and improvement of diagnosis and therapy of relative diseases. In spite of abundant highly sensitive methods that have been developed for the detection of secreted proteins, majority of them fall short in providing sufficient spatial and temporal resolution for comprehensive profiling of protein secretion from single cells. The real-time imaging techniques allow rapid acquisition and manipulation of analyte information on a 2D plane, providing high spatiotemporal resolution. Here, we summarize recent advances in real-time imaging of secretory proteins from single cell, including label-free and labelling techniques, shedding light on the development of simple yet powerful methodology for real-time imaging of single-cell protein secretion.

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Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters

Cited by 22 publications

References 44 publications

Label-free, real-time on-chip sensing of living cancer cell via grating-coupled surface plasmon resonance

Label-free, real-time on-chip sensing of living cancer cell via grating-coupled surface plasmon resonance

On the Use of Surface Plasmon Resonance-Based Biosensors for Advanced Bioprocess Monitoring

Advanced Biosensing towards Real-Time Imaging of Protein Secretion from Single Cells

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