Isolation of Rat Hepatocytes

Fermented Wheat Powder Induces the Antioxidant and Detoxifying System in Primary Rat Hepatocytes

“…The animals were anesthetized with an intraperitoneally injection of Zoletil ® (40 mg/kg) and then subjected to midline laparotomy in order to exteriorize the liver and isolate the portal vein. A needle was inserted into the portal vein and then the liver was perfused as described previously [32].…”

Major Phase I Biotransformation Pathways of Trichostatin A in Rat Hepatocytes and in Rat and Human Liver Microsomes

“…The isolation of rat hepatocytes was performed as described by De Smet et al (1998). Cell integrity was assessed by trypan blue exclusion and only suspensions with a minimum of 84% observed viability were used.…”

Adenoviral gene transfer of ABIN-1 protects mice from TNF/galactosamine-induced acute liver failure and lethality

“…The mouse hepatoma cell line BWTG3 22 was obtained from Dr. C. Szpirer (Free University of Brussels, Brussels, Belgium) and cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum, 2 mmol/L L-glutamine, 1 mmol/L sodium pyruvate, nonessential amino acids, and antibiotics. Primary rat hepatocytes (viability Ͼ 85%) were isolated by a 2-step collagenase perfusion 23 and were plated at a density of 0.4 ϫ 10 5 cells/cm 2 in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum, 2 mmol/L L-glutamine, 1 mmol/L sodium pyruvate, and antibiotics. Four hours after plating, medium was removed and fresh medium, supplemented with 7.5 g/mL hydrocortisone hemisuccinate, was added to the cells.…”