Fermented Wheat Powder Induces the Antioxidant and Detoxifying System in Primary Rat Hepatocytes

Abstract

Many plants exhibit antioxidant properties which may be useful in the prevention of oxidative stress reactions, such as those mediated by the formation of free radical species in different pathological situations. In recent years a number of studies have shown that whole grain products in particular have strong antioxidant activity. Primary cultures of rat hepatocytes were used to investigate whether and how a fermented powder of wheat (Lisosan G) is able to modulate antioxidant and detoxifying enzymes, and whether or not it can activate Nrf2 transcription factor or inhibit NF-kB activation. All of the antioxidant and detoxifying enzymes studied were significantly up-regulated by 0.7 mg/ml Lisosan G treatment. In particular, NAD(P)H:quinone oxidoreductase and heme oxygenase-1 were induced, although to different degrees, at the transcriptional, protein and/or activity levels by the treatment. As for the Nrf2 transcription factor, a partial translocation of its protein from the cytosol to the nucleus after 1 h of Lisosan G treatment was revealed by immunoblotting. Lisosan G was also observed to decrease H2O2-induced toxicityTaken together, these results show that this powder of wheat is an effective inducer of ARE/Nrf2-regulated antioxidant and detoxifying genes and has the potential to inhibit the translocation of NF-kB into the nucleus.

A Fermented Whole Grain Prevents Lipopolysaccharides‐Induced Dysfunction in Human Endothelial Progenitor Cells

“…Not only does this product contain the typical components of cereals but also after fermentation it is enriched in bioactive substances such as phenolic components and alpha-lipoic acid [13], a natural antioxidant that can provide protection against reactive oxygen species- (ROS-) induced vascular damage [14]. In healthy rabbits, LG improves lipid profile and antioxidant status [15], while in in vitro studies LG has been shown to exert protective effects in human microvascular endothelial cells exposed to oxidized-LDL controlling both oxidative and inflammatory processes [16].…”

The effects of fermented wheat powder (Lisosan G) on the blood lipids and oxidative status of healthy rabbits

“…The antioxidant activity of Lisosan G was previously evaluated through in vitro and in vivo experiments (Longo et al, 2007;Frassinetti et al, 2012;La Marca et al, 2013;Longo et al, 2014). Frassinetti et al (2012) demonstrated that Lisosan G removed the free radicals that originated from a normal aerobic metabolism and increased CAT, glutathione peroxidase (GPX) and superoxide dismutase (SOD).…”

“…Ethoxycoumarin-O-deethylase (ECOD), ethoxyresorufin O-deethylase (EROD), catalase (CAT), DT-diaphorase, glutathione-Stransferase (GST) and heme oxygenase-1 (HO-1) activities were measured as reported in La Marca et al (2013) and Vornoli et al (2014).…”

“…The protective effect of Lisosan G could be associated with the reduction of oxidative stress and the preservation of antioxidant enzymes. In particular, in a model of primary rat hepatocytes, it was observed that Lisosan G modulated phase 2 enzymes through the activation of the nuclear factor-erythroid 2-related factor-2 (Nrf2) pathway and decreased the translocation of the nuclear factor kappa B (NF-kB) to the nucleus (La Marca et al, 2013).…”

Nutraceuticals and cardiovascular risk: potential role of EPCs modulation

“…In our work, we explored the effects of Lisosan G. This product not only presents the above mentioned features of cereals, but is also enriched in bioactive compounds such as flavonoids, phenolic components and alpha-lipoic acid, since it derives from a fermentation process [81] . There, we evaluated the viability and cell function of endothelial progenitors and showed beneficial effects on both properties.…”