Isolation of Pure Antibodies

Nezlin, Roald

doi:10.1007/978-1-4684-1791-3_2

Cited by 3 publications

(1 citation statement)

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“…The greater disappearance rate of hp-vs. sp-at-IF-Ab probably reflected prior exposure of hp-ot-IF-Ab to acid during immunoaffinity purification. Acid has been shown to induce alterations in Ab structure that affect metabolic clearance rate [22,23]. Exposure to acid also may have accounted for lower binding affinity of hp-vs. spct-IF-Ab for or-IF [8].…”

Section: Discussionmentioning

confidence: 99%

Effects of Passive Immunization of Ewes Against an Inhibin-Peptide on Gonadotropin Levels, Ovulation Rate, and Prolificacy1

Kusina

Meyer

Carlson

et al. 1995

Biology of Reproduction

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The experimental objectives were to determine whether injection of semi-purified (sp; ammonium sulfate-precipitated) and highly purified (hp; immunoaffinity-purified) ovine antibody (Ab) against an inhibin-peptide fragment (alpha-IF) before the preovulatory period would 1) stimulate FSH secretion in a dose-response manner, 2) induce an increase in ovulation rate, and 3) affect pregnancy rate and prolificacy (lambs born alive per ewe lambing). During the early breeding season, estrus was synchronized in 30 2-yr-old crossbred ewes through use of progesterone-releasing pessaries (CIDR-G). Two doses (330 and 660 laboratory reference preparation [RP-2] kU) of sp- and hp-alpha-IF-Ab were injected i.m. 48 h before CIDR-G removal (6 ewes per group). Six other ewes received control solution. Plasma alpha-IF-Ab titers peaked at 12 h postinjection. Plasma FSH levels were higher (p < 0.02) in alpha-IF-Ab-treated ewes than in control ewes from 12 to 24 h postimmunization. Magnitudes of FSH increases were similar in ewes administered sp- and hp-alpha-IF-Ab and were greater (p < 0.05) in ewes receiving 660 than in those receiving 330 RP-2 kU. Compared to control values, the higher alpha-IF-Ab dose increased FSH levels by 44 +/- 5% and the lower dose increased the levels by 22 +/- 3%. Plasma LH levels were similar among passively immunized and control sheep. Ovulation rate was increased (p < 0.0005) by alpha-IF-Ab treatment.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionmentioning

confidence: 99%