Isolation of oxidase-negative Aeromonas salmonicida from diseased turbot Scophthalmus maximus

Pedersen, Karl; Kofod, Helmer; Dalsgaard, Inger; Larsen, Jesper

doi:10.3354/dao018149

Cited by 46 publications

(48 citation statements)

References 12 publications

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“…Both of the experimental fish pathogen (A. salmonicida and Y. ruckeri) were Gram negative when staining was performed using Gram staining method (Buck, 1982). Several studies have suggested that the A. salmonicida (Pedersen et al, 1994;Gudmundsdottir, 1998;Schmidt et al, 2000) and Y. ruckeri are gram negative fish bacteria (Tinsley et al, 2011;Verner-Jeffreys et al, 2011;Welch et al, 2011) which are reliable with the present results. The oxidase test was used to identify the presence of respiratory enzymes, cytochrome oxidase in the bacterial isolates.…”

Section: Discussionsupporting

confidence: 86%

“…Like oxidase test, A. salmonicida give positive reaction with catalase test. A Positive oxidase and catalase test of A. salmonicida were recoded from turbot fish farm in Denmark (Pedersen et al, 1994;Dalsgaard and Madsen, 2000). The studied A. salmonicida was observed as cytochrome oxidase positive like other research.In contrast, negative oxidase test for Y. ruckeri was recorded from the rainbow trout farm in Spain (Fouz et al, 1990).…”

Section: Discussionmentioning

confidence: 62%

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Morphological and biochemical characterization of two fish pathogenic bacteria: Aeromonas salmonicida and Yersinia ruckeri for rapid diagnosis of fish disease

Akter

2017

Asian J. Med. Biol. Res

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Aeromonas salmonicida and Yersinia ruckeri are two common pathogenic fish bacteria responsible for furunculosis and Enteric Red Mouth disease (ERM), respectively. For the characterization of these two pathogens, a series of morphological (pigmentation, hemolyse, motility and body shape), biochemical tests (Gram staining, catalase, oxidase and API 20E strips) and a soft ionization technique (MALDI-TOF/MS) were performed in the laboratory. Pigmentation, motility, hemolysis and body shape was used as a preliminary identification of the bacteria. Both of the species were identified with the entire biochemical test without any doubt except API20E strips test. Although the API profile of A. salmonicida (0006104) was identified with high confidence (99.6%), but Y. ruckeri was misidentified as Vibrio mimicus. There are strong supports against Vibrio mimicus as it is a human pathogen, grow at a temperature more than 20 o C, motile and oxidase positive bacteria. The Y. ruckeri is a non-motile fish bacteria and oxidase negative which are consistent with the study results. Agglutination test with Bionor Mono kit was also identified the bacteria as Y. ruckeri. For rapid diagnosis of infectious disease, accurate identification of pathogen is very important for commercial aquaculture.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 62%

Morphological and biochemical characterization of two fish pathogenic bacteria: Aeromonas salmonicida and Yersinia ruckeri for rapid diagnosis of fish disease

Akter

2017

Asian J. Med. Biol. Res

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“…Atypical A. salmonicida has been isolated from a number of marine fish species, such as sable fish Anoplopoma fimbria (Evelyn 1971), Atlantic cod (Cornick et al 1984), marine sand eel Ammodytes lancea (Dalsgaard & Paulsen 1986), Pacific herring Clupea harengus pallasi (Traxler & Bell 1989), flounder Platichthys flesus (Wiklund & Bylund 1991), goldsinny (Frerichs et al 1992) and turbot Scophthalmus maximus (Pedersen et al 1994). Typical A. salmonicida (subsp.…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of Atlantic cod Gadus morhua, halibut Hippoglossus hippoglossus and wrasse (Labridae) to Aeromonas salmonicida subsp. salmonicida and the possibility of transmission of furunculosis from farmed salmon Salmo salar to marine fish

Hjeltnes¹,

Bergh²,

Wergeland³

et al. 1995

Dis. Aquat. Org.

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“…The farming of these 3 species has already encountered problems with several bacterial infections, such as vibrio infections (Vibrio anguillarum and Vibrio vulnificus) and atypical Aeromonas salmonicida infections (Mellergaard & Dalsgaard 1987, Noga et al 1990, Biosca et al 1991, Fouz et al 1992, 1995, Austin et al 1993, Liewes & Haenen 1993, Pedersen et al 1994, Grisez et al 1996, Larsen & Pedersen 1996, Bergh et al 1997, Olsson et al 1998, Bricknell et al 1999, 2000, Dalsgaard et al 1999, Gatesoupe et al 1999.…”

Section: Introductionmentioning

confidence: 99%

Disposition of 14C-flumequine in eel Anguilla anguilla, turbot Scophthalmus maximus and halibut Hippoglossus hippoglossus after oral and intravenous administration

Hansen

Ingebrigtsen²,

Wl³

et al. 2001

Dis. Aquat. Org.

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The absorption, distribution and elimination of 14 C-labelled flumequine were studied using whole body autoradiography and liquid scintillation counting. Flumequine was administered to eel Anguilla anguilla, turbot Scophthalmus maximus and halibut Hippoglossus hippoglossus intravenously and orally as a single dose of 5 mg kg . The turbot and halibut studies were performed in salt water (salinity of 32 ‰) at temperatures of 16 ± 1°C (turbot) and 9.5 ± 0.5°C (halibut). The eel study was conducted in fresh water at 23 ± 1°C. In the intravenously administered groups flumequine was rapidly distributed to all major tissues and organs. After oral administration flumequine also appeared to have rapid and extensive absorption and distribution in all 3 species. After the distribution phase, the level of flumequine was higher in most organs and tissues than in the blood, except in muscle and brain. The most noticeable difference between the species was the slow elimination of flumequine from eel compared to turbot and halibut. In orally administered eels, substantial amounts of flumequine remained in all major organs/tissues for 7 d. At 28 d significant levels of flumequine were present in liver, kidney and skin (with traces in muscle), and at the last sampling point (56 d) in eye, bone, bile and posterior intestine. In orally administered turbot significant levels of flumequine were observed over 96 h in bile, urine, bone, skin, intestine and eye, and traces were detected over 28 d in bone and eye in addition to a significant level in bile. In orally administered halibut, significant levels of flumequine were observed in bile, skin, intestine and eye over 96 h. Traces were present in skin and eye over 7 d. The maximal flumequine concentrations in blood were calculated to be 2.5 mg equivalents l -1 (eel at 12 h), 0.8 mg l -1 (turbot at 6 h) and 0.6 mg l -1 (halibut at 6 h) after oral administration.

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Isolation of oxidase-negative Aeromonas salmonicida from diseased turbot Scophthalmus maximus

Cited by 46 publications

References 12 publications

Morphological and biochemical characterization of two fish pathogenic bacteria: Aeromonas salmonicida and Yersinia ruckeri for rapid diagnosis of fish disease

Morphological and biochemical characterization of two fish pathogenic bacteria: Aeromonas salmonicida and Yersinia ruckeri for rapid diagnosis of fish disease

Susceptibility of Atlantic cod Gadus morhua, halibut Hippoglossus hippoglossus and wrasse (Labridae) to Aeromonas salmonicida subsp. salmonicida and the possibility of transmission of furunculosis from farmed salmon Salmo salar to marine fish

Disposition of 14C-flumequine in eel Anguilla anguilla, turbot Scophthalmus maximus and halibut Hippoglossus hippoglossus after oral and intravenous administration

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