Isolation of oryzanol from crude rice bran oil

Zullaikah, Siti; Melwita, Elda; Ju, Yi‐Hsu

doi:10.1016/j.biortech.2008.06.008

Cited by 59 publications

(46 citation statements)

References 11 publications

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“…Nearly 1.9% of oryzanol rich concentrate was recovered after crystallizing the distillate at -60 ºC for 15 hours. The percentage of oryzanol reported here is slightly lower than the earlier report (Zullaikah et al, 2009). It may be due to the loss of γ-oryzanol during the different steps of refining prior to deodorization.…”

Section: Yield Of Oryzanol Rich Concentrate During Crystallizationcontrasting

confidence: 84%

“…Some impurities which remained in the form of gums and waxes at the time of refining were removed by following the process described earlier with slight modification in terms of raw material (Zullaikah et al, 2009). After that rice bran oil distillate rich in γ-oryzanol was taken as raw material for the two steps crystallization as mentioned by Zullaikah et al, 2009.…”

Section: Gums Waxes Removal and Crystallizationmentioning

confidence: 99%

“…After that rice bran oil distillate rich in γ-oryzanol was taken as raw material for the two steps crystallization as mentioned by Zullaikah et al, 2009. Oryzanol rich concentrate obtained was further purified through column chromatography to get pure γ-oryzanol.…”

Section: Gums Waxes Removal and Crystallizationmentioning

confidence: 99%

“…γ-oryzanol was recovered from the rice bran oil distillate by following the process of Zullaikah et al, 2009. Nearly 1.9% of oryzanol rich concentrate was recovered after crystallizing the distillate at -60 ºC for 15 hours.…”

Section: Yield Of Oryzanol Rich Concentrate During Crystallizationmentioning

confidence: 99%

See 3 more Smart Citations

Rice Bran Oil Distillate, a Choice for Gamma-Oryzanol: Separation and Oxidative Stability Study

Jaiswal¹,

Pradhan²,

Patel³

et al. 2014

JFR

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Rice bran oil distillate is one among the secondary products produced during refining of rice bran oil. Rice bran oil distillate is a source of several micronutrients and natural antioxidants like γ-oryzanol, tocopherols etc. The aim of the present study was to separate γ-oryzanol from distillate and utilize it as a stabilizer for edible oil. In order to achieve this aim crystallization process was applied to obtain oryzanol rich concentrate. Further purification of oryzanol was achieved through column chromatography. Fractions of γ-oryzanol were quantified through HPLC which gives 0.83% yield. Separated γ-oryzanol was used to study the stability of pea nut and linseed oil using rancimat. The experiments were carried out in rancimat at varying temperature (110-130 ºC) and concentration (100-300 ppm). Stability of both the oil was found to be directly proportional to the γ-oryzanol concentration and inversely proportional to the temperature.

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Section: Yield Of Oryzanol Rich Concentrate During Crystallizationcontrasting

confidence: 84%

Section: Gums Waxes Removal and Crystallizationmentioning

confidence: 99%

Section: Gums Waxes Removal and Crystallizationmentioning

confidence: 99%

Section: Yield Of Oryzanol Rich Concentrate During Crystallizationmentioning

confidence: 99%

See 2 more Smart Citations

Rice Bran Oil Distillate, a Choice for Gamma-Oryzanol: Separation and Oxidative Stability Study

Jaiswal¹,

Pradhan²,

Patel³

et al. 2014

JFR

View full text Add to dashboard Cite

show abstract

“…For instance, vitamin E tocopherols and tocotrienols is concentrated mainly in deodorized rice bran scum oil but not in commercial rice bran oil 18 . Degumming and dewaxing has been reported to remove 1.1 and 5.9 of γ-oryzanols from the original crude oil 19 .…”

Section: Rice Bran Oilmentioning

confidence: 99%

Supercritical CO<sub>2</sub> Extraction of Rice Bran Oil –the Technology, Manufacture, and Applications

Sookwong

Mahatheeranont

2017

J. Oleo Sci.

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One‐step isolation of γ‐oryzanol from rice bran oil by non‐aqueous hydrostatic countercurrent chromatography

Angelis

Urbain

Halabalaki

et al. 2011

J of Separation Science

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The value-added γ-oryzanol was purified in one step from crude rice bran oil (RBO) using a preparative hydrostatic countercurrent chromatography (hydrostatic CCC) method, operating in the dual mode. The fractionation was performed using a non-aqueous biphasic solvent system consisting of heptane-acetonitrile-butanol (1.8:1.4:0.7, v/v/v), leading rapidly to the target compounds. Transfer of the analytical CCC method to large-scale isolation was also carried out yielding a high quantity-high purity fraction of γ-oryzanol. In addition, a fraction of hydroxylated triterpene alcohol ferulates (polar γ-oryzanol) was clearly separated and obtained. Furthermore, a fast HPLC-APCI(±)-HRMS method was developed and applied for the identification of γ-oryzanol as well as the polar γ-oryzanol in RBO and the resulting fractions. The purity of γ-oryzanol fraction was estimated as 97% based on HPLC-APCI-HRMS analysis.

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Isolation of oryzanol from crude rice bran oil

Cited by 59 publications

References 11 publications

Rice Bran Oil Distillate, a Choice for Gamma-Oryzanol: Separation and Oxidative Stability Study

Rice Bran Oil Distillate, a Choice for Gamma-Oryzanol: Separation and Oxidative Stability Study

Supercritical CO<sub>2</sub> Extraction of Rice Bran Oil –the Technology, Manufacture, and Applications

One‐step isolation of γ‐oryzanol from rice bran oil by non‐aqueous hydrostatic countercurrent chromatography

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