Rice Bran Oil Distillate, a Choice for Gamma-Oryzanol: Separation and Oxidative Stability Study

Abstract

Rice bran oil distillate is one among the secondary products produced during refining of rice bran oil. Rice bran oil distillate is a source of several micronutrients and natural antioxidants like γ-oryzanol, tocopherols etc. The aim of the present study was to separate γ-oryzanol from distillate and utilize it as a stabilizer for edible oil. In order to achieve this aim crystallization process was applied to obtain oryzanol rich concentrate. Further purification of oryzanol was achieved through column chromatography. Fractions of γ-oryzanol were quantified through HPLC which gives 0.83% yield. Separated γ-oryzanol was used to study the stability of pea nut and linseed oil using rancimat. The experiments were carried out in rancimat at varying temperature (110-130 ºC) and concentration (100-300 ppm). Stability of both the oil was found to be directly proportional to the γ-oryzanol concentration and inversely proportional to the temperature.