Isolation of novel chitinolytic bacteria and production optimization of extracellular chitinase

Process Optimization on Chitinase Production by Locally Isolated Enterobacter sp. and Zymomonas sp.

“…growth well under slight acidic condition that was at pH 6 with the chitinase production of 5.08 ± 0.17 U/mL. Some microorganisms are able to produce high amount of chitinase under alkaline condition [3], [5], [20], while some microorganism preferable acidic condition for chitinase production [22], [24]. This can conclude that pH plays an important role production of chitinase.…”

“…Besides, different bacteria strain has different incubation time for high production of chitinase. After 24 h of fermentation, [5] found out that highest chitinase could be obtained by Aeromonas hydrophila. However Aeromonas punctata produced the highest chitinase production after 48 h [5].…”

“…After 24 h of fermentation, [5] found out that highest chitinase could be obtained by Aeromonas hydrophila. However Aeromonas punctata produced the highest chitinase production after 48 h [5]. For examples, [16] reported that after 48 h of incubation highest chitinase production was obtained from Bacillus cereus, B. alvei and B. sphaericus.…”

“…The modified method of [5] was used to prepare the colloidal chitin using chitin powder from crab shells (Malacai tesque, Japan), where 5 g of the chitin powder was treated with 75 mL of concentrated HCl and vigorous stirred at 30 °C for 60 min. The mixture was then treated with 2 L of cold distilled water (∼4 °C) and centrifugation at 12,000 rpm for 25 min to obtain precipitated colloidal chitin.…”

“…4). Different microorganism has different optimum temperature for its growth and chitinase production [5], [22], [23]. Based on Fig.…”