Isolation of new pregnancy-associated glycoproteins from water buffalo (Bubalus bubalis) placenta by Vicia villosa affinity chromatography

Barbato, Olimpia; Sousa, Noelita Melo de; Klisch, Karl; Clerget, E.; Debenedetti, A.; Barile, V. L.; Malfatti, Alessandro; Beckers, Jean‐François

doi:10.1016/j.rvsc.2008.01.004

Cited by 31 publications

(35 citation statements)

References 46 publications

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“…PAGs are abundantly expressed products of the placenta of species within the Cetartiodactyla order (even-toed ungulates; Szafranska et al 1995, Xie et al 1997, Garbayo et al 2000, Green et al 2000, Brandt et al 2007, Barbato et al 2008, Majewska et al 2011, Bériot et al 2014. They are represented in current sequence databases comprising Suidae (e.g.…”

Section: The Pag Familymentioning

confidence: 99%

“…Another possibility proposed by Wooding et al (2005) was that PAGs accumulating at the placenta-uterine interface might play a role in adhesion by acting as bridging molecules. The PAGs possess a peptide/substrate-binding cleft as well as N-and O-linked carbohydrate side chains (Xie et al 1991, Green et al 1998, Klisch et al 2008. Conceivably, the binding cleft could bind with a protein, such as an integrin, and the carbohydrates displayed on the PAGs could bind a lectin.…”

Section: Possible Functions Of the Pagsmentioning

confidence: 99%

“…Finally, a PAG-associated immunomodulatory role may be due to the glycosylation moieties displayed on the PAGs. PAGs purified from mid-gestation placenta possess a major carbohydrate that consisted of a tetra-antennary glycan with bisecting N-acetylglucosamine with terminal Sd a antigens (Klisch et al 2008). This glycan could provide some degree of protection to the placental semi-allograft.…”

Section: Proposed Immunomodulatory Actions Of Pag1/pspbmentioning

confidence: 99%

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Placental PAGs: gene origins, expression patterns, and use as markers of pregnancy

et al. 2015

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Pregnancy-associated glycoproteins (PAGs) are abundantly expressed products of the placenta of species within the Cetartiodactyla order (even-toed ungulates). They are restricted to this order and they are particularly numerous in the Bovidae. The PAGs exhibit a range of temporal and spatial expression patterns by the placental trophoblasts and probably represent a group of related proteins that perform a range of distinct functions in the epitheliochorial and synepitheliochorial placental forms. This review presents an overview of the origins of the PAGs, a summary of PAG expression patterns, and their use as markers of pregnancy status. Speculations about their putative role(s) in pregnancy are also presented.Reproduction (2015) 149 R115-R126

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Section: The Pag Familymentioning

confidence: 99%

Section: Possible Functions Of the Pagsmentioning

confidence: 99%

Section: Proposed Immunomodulatory Actions Of Pag1/pspbmentioning

confidence: 99%

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Placental PAGs: gene origins, expression patterns, and use as markers of pregnancy

et al. 2015

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“…After washing with PBS Tween 0.1%, Western analysis by alkaline phosphatase (1: 5000) was incubated at 37 o C for 60 minutes. After three times washing, nitrotetrazolium blue chlorine-98% (NBT) and 5-bromo-4-chloro-3-indolyl phosphate disodium salt (BICP) were added as standard substrates, then was incubated for 20 minutes, and washed again with dH 2 O (Goldsby et al, 2000;Barbato et al, 2007;Bella et al, 2009;Majewska et al, 2005;Huebner, 2004).…”

Section: Rabbit Anti-ovpag Determination Using Western Blotting Technmentioning

confidence: 99%

“…Rabbit antiovPAG determined using monogel SDS-PAGE method followed with Western Blot. This method was applied for detecting the polyclonal antibody in blood (Barbato et al, 2007;Bella et al, 2009) whereas its concentration was measured using modified ELISA technique. The aim of the study was to produce polyclonal antibody (rabbit anti-ovPAG) which could detect PAG in the urine of pregnant ewes…”

Section: Introductionmentioning

confidence: 99%

ANTIBODY POLYCLONAL PRODUCTION ON RABBIT ANTI-OVINE PREGNANCY-ASSOCIATED GLYCOPROTEIN (Rabbit anti-ovPAG)

Setiatin

Sajuthi

Purwantara

et al. 2011

J. Indonesian Trop. Anim. Agric.

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The aim of the study was to produce polyclonal antibody (rabbit anti-ovPAG) which could detect PAG in the urine of pregnant ewes. Twelve rabbits were immunized against ovPG DEAE-TrisHCl (DT), DEAE-NaCl 20mM (DN2), DEAE-NaCl 40mM (DN4), DEAE-NaCl 80mM (DN8), DEAE-NaCl 160mM (DN16), DEAE-NaCl 320mM (DN32) and DEAE-NaCl 1M (DN1) and NaCl 0.9 % as a placebo. The 0.5 ml of isolate (purified from ovine cotyledon) was emulsified in equal volume with complete and incomplete Freud's adjuvant. The mixture of each isolate and adjuvant was injected at mutiple sites along the dorsal area of rabbits by subcutaneous route. Blood were collected from marginal ear vein, starting before first injection (baseline) and every 14 days. Rabbit anti-ovPAG were measured using Modified ELISA Technique. By using Western Blot Technique, DN32 showed the best immune response among others and also could differenciate ovPAG in the urine of pregnant ewes It could be concluded that ovPAG DN32 is a specific source of rabbit anti-ovPAG production. Protein of ovPAG at molecular weight 31 kDa is a pregnancy protein marker of garut sheep and could be developed as a major protein for producing antibodi.

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Protein Signatures of Lactation and Early Pregnancy Diagnosis in Buffalo (Bubalus bubalis)

Jena

Mohanty

2022

Biotechnological Applications in Buffalo Research

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Isolation of new pregnancy-associated glycoproteins from water buffalo (Bubalus bubalis) placenta by Vicia villosa affinity chromatography

Cited by 31 publications

References 46 publications

Placental PAGs: gene origins, expression patterns, and use as markers of pregnancy

Placental PAGs: gene origins, expression patterns, and use as markers of pregnancy

ANTIBODY POLYCLONAL PRODUCTION ON RABBIT ANTI-OVINE PREGNANCY-ASSOCIATED GLYCOPROTEIN (Rabbit anti-ovPAG)

Protein Signatures of Lactation and Early Pregnancy Diagnosis in Buffalo (Bubalus bubalis)

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