Isolation of NEB-LFamide, a novel myotropic neuropeptide from the grey fleshfly

Janssen, Ine; Schoofs, Liliane; Spittaels, Kurt; Neven, Hedwig; Broeck, Jozef Vanden; Devreese, Bart; Beeumen, Jozef Van; Shabanowitz, J.; Hunt, Donald F.; Loof, Arnold De

doi:10.1016/0303-7207(95)03746-2

Cited by 51 publications

(27 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A peptide of the IFamide family was initially isolated from 350 000 adult gray flesh flies 54 as a factor stimulating the spontaneous contractions of a locust oviduct. Both the sequence and immunoreactive distribution pattern of IFamides are very conserved in all insects examined so far.…”

Section: Ifamide Precursormentioning

confidence: 99%

Peptidomic analysis of the larval Drosophila melanogaster central nervous system by two‐dimensional capillary liquid chromatography quadrupole time‐of‐flight mass spectrometry

et al. 2005

View full text Add to dashboard Cite

Peptides are the largest class of signalling molecules found in animals. Nevertheless, in most proteomic studies peptides are overlooked since they literally fall through the mazes of the net. In analogy with proteomics technology, where all proteins expressed in a cell or tissue are analyzed, the peptidomic approach aims at the simultaneous visualization and identification of the whole peptidome of a cell or tissue, i.e. all expressed peptides with their post-translational modifications. In this paper we describe the analysis of the larval fruit fly central nervous system using two-dimensional capillary liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (LC/Q-TOF-MS/MS. Using the central nervous systems of only 50 larval Drosophila as starting material, we identified 38 peptides in a single analysis, 20 of which were not detected in a previous study that reported on the one-dimensional capillary LC/MS/MS analysis of the same tissue. Among the 38 sequenced peptides, some originate from precursors, such as the tachykinin and the IFamide precursor that were entirely missed in the first study. This clearly demonstrates that the two-dimensional capillary LC approach enhances the coverage of the peptidomic analysis.

show abstract

Section: Ifamide Precursormentioning

confidence: 99%

Peptidomic analysis of the larval Drosophila melanogaster central nervous system by two‐dimensional capillary liquid chromatography quadrupole time‐of‐flight mass spectrometry

et al. 2005

View full text Add to dashboard Cite

show abstract

“…The SIFa family was initially isolated as a myotropic peptide from the grey fleshfly Neobellieria bullata (Janssen et al, 1996). The structure of the mature form of SIFa (AYRKPPFNGSIFamide) and its distribution are well conserved in many insect species (Broeck, 2001; Verleyen et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

SIFamide and SIFamide Receptor Define a Novel Neuropeptide Signaling to Promote Sleep in Drosophila

Park¹,

Sonn²,

Oh³

et al. 2014

Molecules and Cells

View full text Add to dashboard Cite

SIFamide receptor (SIFR) is a Drosophila G protein-coupled receptor for the neuropeptide SIFamide (SIFa). Although the sequence and spatial expression of SIFa are evolutionarily conserved among insect species, the physiological function of SIFa/SIFR signaling remains elusive. Here, we provide genetic evidence that SIFa and SIFR promote sleep in Drosophila. Either genetic ablation of SIFa-expressing neurons in the pars intercerebralis (PI) or pan-neuronal depletion of SIFa expression shortened baseline sleep and reduced sleep-bout length, suggesting that it caused sleep fragmentation. Consistently, RNA interference-mediated knockdown of SIFR expression caused short sleep phenotypes as observed in SIFa-ablated or depleted flies. Using a panel of neuron-specific Gal4 drivers, we further mapped SIFR effects to subsets of PI neurons. Taken together, these results reveal a novel physiological role of the neuropeptide SIFa/SIFR pathway to regulate sleep through sleep-promoting neural circuits in the PI of adult fly brains.

show abstract

“…However, in contrast to the wealth of information on the hormonal contents of insect midgut endocrine cells, nothing is known about the identity of the paracrines/hormones present in the putative midgut epithelial endocrine cells of any decapod species. Here, we have begun an immunohistochemical and mass spectrometric investigation to determine the extent to which crustacean neuropeptide paracrines and hormones are located in and released by midgut epithelial endocrine cells of Cancer species, focusing on the tachykinin-related peptides (TRPs), which are welldocumented brain-gut peptides in insects (reviewed by Nässel, 1999), and the SIFamides, a newly described family of neuropeptides present in both insects and crustaceans (Janssen et al, 1996;Vanden Broeck, 2001;Huybrechts et al, 2003;Sithigorngul et al, 2002;Verleyen et al, 2004;Yasuda et al, 2004;Messinger et al, 2005;Christie et al, 2006). Some of these data have appeared previously in abstract form .…”

Section: Introductionmentioning

confidence: 99%

Midgut epithelial endocrine cells are a rich source of the neuropeptides APSGFLGMRamide (Cancer borealis tachykinin-related peptide Ia) and GYRKPPFNGSIFamide (Gly1-SIFamide) in the crabs Cancer borealis, Cancer magister and Cancer productus

Christie

Kutz-Naber

Stemmler

et al. 2007

Journal of Experimental Biology

View full text Add to dashboard Cite

-SIFamide) and APSGFLGMRamide [Cancer borealis tachykinin-related peptide Ia (CabTRP Ia)], respectively, both of which are known neuropeptides of Cancer species. Although the function of these midgut-derived peptides remains unknown, we found that both Gly 1 -SIFamide and CabTRP Ia were released when the midgut was exposed to highpotassium saline. In addition, CabTRP Ia was detectable in the hemolymph of crabs that had been held without food for several days, but not in that of fed animals, paralleling results that were attributed to TRP release from midgut endocrine cells in insects. Thus, one function that midgutderived CabTRP Ia may play in Cancer species is paracrine/hormonal control of feeding-related behavior, as has been postulated for TRPs released from homologous cells in insects.

show abstract

Isolation of NEB-LFamide, a novel myotropic neuropeptide from the grey fleshfly

Cited by 51 publications

References 21 publications

Peptidomic analysis of the larval Drosophila melanogaster central nervous system by two‐dimensional capillary liquid chromatography quadrupole time‐of‐flight mass spectrometry

Peptidomic analysis of the larval Drosophila melanogaster central nervous system by two‐dimensional capillary liquid chromatography quadrupole time‐of‐flight mass spectrometry

SIFamide and SIFamide Receptor Define a Novel Neuropeptide Signaling to Promote Sleep in Drosophila

Midgut epithelial endocrine cells are a rich source of the neuropeptides APSGFLGMRamide (Cancer borealis tachykinin-related peptide Ia) and GYRKPPFNGSIFamide (Gly1-SIFamide) in the crabs Cancer borealis, Cancer magister and Cancer productus

Contact Info

Product

Resources

About