Isolation of Myrosinase Producing Microorganism

Tani, Naoki; Ohtsuru, Masaru; Hata, Toshiyuki

doi:10.1080/00021369.1974.10861387

Cited by 36 publications

(40 citation statements)

References 2 publications

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“…The Michaelis constant varied over a 10-fold range depending on the glucusinolate but were in accordance with values (using sinigrin) for purified enzymes from Enterobacter cloacae enzyme (0.37 mM) (Tani et al, 1974), B. napus and S. alba (0.03 to 0.17 mM, respectively) (Bones and Rossiter, 1996) and W. japonica (0.47 mM) (Ohtsuru and Kawatani, 1979).…”

Section: Discussionsupporting

confidence: 54%

Characterisation of aphid myrosinase and degradation studies of glucosinolates

Francis

Lognay

Wathelet

et al. 2002

Arch Insect Biochem Physiol

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Myrosinase from Brevicoryne brassicae was purified by ammonium sulfate fractionation, dialysis, and chromatography on a DEAE column. The chromatography yielded a single peak and a 115.6-fold purification. Further FPLC gel filtration gave a single peak at 120 kDa. Denaturing SDS/PAGE of the protein revealed a single band at 60 kDa, indicating that the native B. brassicae myrosinase is a dimer. Kinetic parameters towards 8 glucosinolates were calculated. Strong differences of V(max) and K(m) were observed depending on the substrate. Degradation products of each glucosinolate were identified and quantified by GC-MS and GLC-FID, respectively. Using both crude aphid homogenates and purified myrosinase, two unique hydroxyglucosinolates, 3-butenyl- and benzyl-isothiocyanates were identified from progoitrin ((2S)-2-hydroxybut-3-enyl-glucosinolate) and sinalbin (4-hydroxybenzyl-glucosinolate) degradation respectively. Addition of ascorbic acid to the reaction mixtures containing sinalbin and progoitrin caused the production of hydroxylated degradation products usually associated with plant myrosinase metabolisation. The occurrence of the myrosinase system in B. brassicae is discussed in terms of similar allelochemical adaptation between the herbivore and its host plant.

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Section: Discussionsupporting

confidence: 54%

Characterisation of aphid myrosinase and degradation studies of glucosinolates

Francis

Lognay

Wathelet

et al. 2002

Arch Insect Biochem Physiol

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“…Further conversion of several GSL derivatives into unknown compounds has been demonstrated in sheep rumen fluid in vitro (Lanzani et al 1974;Duncan & Milne, 1992). There is little other information: the salient points in the studies of Oginsky et al (1965) and Tani et al (1974) are the influence of pH and the lack of influence, or even inhibitory effect, of ascorbic acid on bacterial myrosinase-like activities in vitro. In vivo, manipulation of the mineral or carbohydrate fraction of the diet helped to reduce the biological effects of cruciferous vegetables, implying that the bacterial myrosinase-like activities were altered in some way.…”

Section: Bacterial Metabolism Of Glucosinolatesmentioning

confidence: 99%

“…Subsequently, different groups succeeded in isolating from human (Oginsky et al 1965;Tani et al 1974) and fowl (Miguchi et al 1974) faecal microflora bacterial strains that were able to metabolize progoitrin or sinigrin in vitro.…”

Section: Bacterial Metabolism Of Glucosinolatesmentioning

confidence: 99%

Glucosinolates and Glucosinolate Derivatives: Implications for Protection Against Chemical Carcinogenesis

Nugon-Baudon¹,

Rabot²

1994

Nutr. Res. Rev.

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* Corresponding author. Cruciferae Brassica oleracea L. gongylodes group capitata group sabauda group gemmifera group italica group botrytis group var. caulifora DC. var. cymosa Lam. var. millecapitata (Lev) Thell. var. medullosa Thell. var. selensia var. sabellica acephala group Brassica alboglabra Bailey Brassica pekinensis (Lour.) Rupr. Brassica chinensis L. Brassica campestris L. ssp. rapifera (Metzg.) Sinsk. ssp. oleijera (Metzg.) Sinsk. var. napobrassica (L.) Peterm var. napus Brassica napus L. or ssp. rapifera (Metzg.) Sinsk.

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“…Microbial degradation of glucosinolates might also be possible without myrosinase as the initial hydrolytic step. Tani et al (1974) reported the degradation of sinigrin by Agrobacterium tumefaciens and Sarcina lutea without detectable accumulation of known hydrolysis products. Most fungi are capable of growing on solid materials without the presence of free liquid (solid-state fermentation, SSF).…”

Section: Introductionmentioning

confidence: 99%

Glucosinolate degradation by Aspergillus clavatus and Fusarium oxysporum in liquid and solid-state fermentation

Smits

Knol

Bol

1993

Appl Microbiol Biotechnol

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Two fungal strains, Aspergillus clavatus and Fusarium oxysporum @ 149, proved to be capable of degrading sinigrin and sinalbin. During the degradation of sinigrin by whole cells of the Aspergillus strain, allylcyanide accumulated in the liquid incubation mixture. After a maximum concentration had been reached, the concentration of allylcyanide decreased as a result of its instability in the medium used. Incubation of cellfree extracts with sinigrin resulted in accumulation of glucose and allylisothiocyanate, suggesting that myrosinase is involved. Experiments with intact ceils and cellfree extracts indicate the formation of an as yet unknown intermediate. When sinigrin was degraded by the AspergiIlus strain in mustard seed meal under solid-state fermentation (SSF) conditions, no accumulation of allylcyanide or allylisothiocyanate was measured. Degradation of sinigrin by F. oxysporum @ 149 did not result in accumulation of intermediates, neither in liquid incubation mixtures nor in mustard seed meal under SSF conditions. Sinigrin was not degraded during incubation with cell-free extracts of F. oxysporum @ 149. Degradation of sinalbin by A. clavatus and F. oxysporum was measured during fermentation of yellow mustard seed meal under SSF conditions. Both fungi are useful for laboratory-scale SSF of mustard seed meal, thus opening new perspectives for a cost effective detoxification process for raw feed materials.

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Isolation of Myrosinase Producing Microorganism

Cited by 36 publications

References 2 publications

Characterisation of aphid myrosinase and degradation studies of glucosinolates

Characterisation of aphid myrosinase and degradation studies of glucosinolates

Glucosinolates and Glucosinolate Derivatives: Implications for Protection Against Chemical Carcinogenesis

Glucosinolate degradation by Aspergillus clavatus and Fusarium oxysporum in liquid and solid-state fermentation

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