Isolation of Mycoplasma bovis from bovine clinical mastitis cases in Northern Greece

Filioussis, George; Christodoulopoulos, G.; Thatcher, Alan; Petridou, V.; Bourtzi-Chatzopoulou, E.

doi:10.1016/j.tvjl.2005.08.001

Cited by 28 publications

(25 citation statements)

References 16 publications

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“…Dessas amostras apenas uma foi positiva na PCR para Mycoplasma bovis com uma prevalência de 1,4% do agente na região em estudo. Prevalências igualmente baixas foram encontradas em estudos de amostras de leite de tanques em outros países como 5,4% na Grécia (Filioussis et al 2007), 1 a 8% nos Estados Unidos , 1,8% na Thailândia (Kampa et al 2009) e 1,5% na Bélgica (Passchyn et al 2012) não tendo sido isolado em estudo realizado na Nova Zelândia (McDonald et al 2009). …”

Section: Resultsunclassified

Prevalência de Mycoplasma bovis em rebanhos de vacas leiteiras

et al. 2018

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RESUMO: Mycoplasma é um patógeno altamente contagioso, podendo causar mastite, pneumonia, artrite, entre outras enfermidades. Seu isolamento requer meios e condições específicas devido ao seu crescimento fastidioso. Devido à complexidade do seu diagnóstico, acredita-se que a real prevalência de casos de mastite por micoplasma seja subestimada. O objetivo do presente estudo foi identificar a prevalência de Mycoplasma bovis em diferentes rebanhos de bovinos leiteiros no estado de São Paulo. O estudo foi dividido em fase de triagem, na qual colheram-se amostras de 67 tanques de expansão e a coleta individual, na qual propriedades positivas para M. bovis foram visitadas e colhidas amostras de leite de todos os animais com mastite clínica e subclínica. O diagnóstico laboratorial foi feito por meio da PCR e cultivo microbiológico específico. A prevalência de M. bovis encontrada na fase de triagem foi de 1,4%. Na fase individual, todas as amostras de leite, procedentes de propriedade positiva para M. bovis no tanque de expansão, foram negativas, o que permite concluir pela baixa prevalência do agente nas condições do presente estudo.

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Section: Resultsunclassified

Prevalência de Mycoplasma bovis em rebanhos de vacas leiteiras

et al. 2018

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“…Filioussis et al (9) published a similar report after isolating M. bovis in 8.2% of dairy cows with mastitis. All of the infected cows had been imported to Greece from European countries.…”

Section: Discussionmentioning

confidence: 79%

“…The incidence of Mycoplasma infections is reported to be increasing in cattle in European countries, Canada, and the USA (9). The agent is transmitted to countries that are free from infection by cattle imports (9,10).…”

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confidence: 99%

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Mycoplasma infections in dairy cattle farms in Turkey

Sayın¹,

Sakmanoğlu²,

Uçan³

et al. 2016

Turk J Vet Anim Sci

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“…Numerous microorganisms are known to cause mastitis, but the major mastitis causal agents are S. aureus, S. agalactiae, S. dysagalactiae, Streptococcus uberis and Mycoplasma bovis (Leitner et al, 2003;Grőhn et al, 2004;Filioussis et al, 2007;Fadlelmula et al, 2009). Among these pathogens, S. aureus is one of the most frequent causes of subclinical and clinical bovine mastitis (Leitner et al, 2003;Brouillette and Malouin, 2005).…”

Section: Introductionmentioning

confidence: 99%

The antimicrobial activity of Enhanced Virgin Coconut Oil (EVCO) on growth of mastitis pathogens

Koh¹,

Long²

2014

MJM

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Aims:The effect of EVCO, containing about 57.48% triglycerides, 26.88% diglycerides, 1.51% monoglycerides and 14.13% free fatty acids, against clinical mastitis pathogens, bought from American Type Cultures Collection (ATCC), was investigated. The present study aims to determine the efficacy of EVCO against three potent mastitis causal agents, namely S. aureus (ATCC 31885), S. agalactiae (ATCC 12927) and S. dysagalactiae (ATCC 27957). Methodology and results:The In-vitro study showed that EVCO can act as a potent agent to inhibit the growth of Staphylococcus aureus (ATCC 31885), Streptococcus agalactiae (ATCC 12927) and Streptococcus dysgalactiae (ATCC 27957). A time kill study showed that EVCO at 2.5% is able to kill both Streptococcus spp. (ATCC 12927 & ATCC 27957) in 5 min of incubation time. Among three mastitis pathogens, S. aureus was the most difficult to eradicate and required at least 5% EVCO for 100% inhibition after 30 min of treatment. The In-vivo study on mastitis-induced lactating cows showed the amount of EVCO introduced into the infected mammary gland was considered over dosage; 50% concentration of EVCO was over dosage, even though it reduced the growth of pathogens from 10 4 to 10 0 . The acidic characteristics of EVCO caused the protein in milk to clot and the udders to swell, even after several days of treatment. Conclusion, significance and impact study: The EVCO had shown its antimicrobial efficacy against three potent mastitis causal agents. The amount of EVCO used to treat mastitis-induced cows and the number of treatments applied needed to be reduced to avoid the milk clotting and udders swelling as a result of the acidic characteristics of EVCO.

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Isolation of Mycoplasma bovis from bovine clinical mastitis cases in Northern Greece

Cited by 28 publications

References 16 publications

Prevalência de Mycoplasma bovis em rebanhos de vacas leiteiras

Prevalência de Mycoplasma bovis em rebanhos de vacas leiteiras

Mycoplasma infections in dairy cattle farms in Turkey

The antimicrobial activity of Enhanced Virgin Coconut Oil (EVCO) on growth of mastitis pathogens

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