Isolation of mutants with improved production of cAMP from Microbacterium sp. no. 205 (ATCC21376)

Ishiyama, Jiro

doi:10.1007/bf00170060

Cited by 15 publications

(4 citation statements)

References 12 publications

(11 reference statements)

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“…Many experiments have shown that cAMP has multiple pharmacological actions such as relaxing smooth muscle, expanding blood vessel, improving liver function and promoting nerve regeneration (Hong and Peng 2003;Tsai et al 2004). Microbial production of cAMP has attracted much attention because it is simple and economical compared to the complicated multistep chemical synthesis (Ishiyama 1990).…”

Section: Introductionmentioning

confidence: 99%

Dynamic mathematical models of batch experiments and fed-batch cultures for cyclic adenosine monophosphate production by Arthrobacter A302

Chen

Ren

et al. 2011

World J Microbiol Biotechnol

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A glucose utilizing strain, Arthrobacter A302 was used for cyclic adenosine monophosphate (cAMP) production in batch modes. The non-structured model in a 5 l stirred tank bioreactor for understanding, controlling, and optimizing the fermentation process was proposed using the logistic equation for microbial growth, the LuedekingPiret equation for product formation and LuedekingPiret-like equation for substrate uptake, respectively. The production of cAMP was a mixed-growth-associated pattern. Based on model prediction, a comparison of calculated value using the parameters evaluated above with another experimental data in 30 l bioreactor was used to test the model. The results predicted from the model were in good agreement with the experimental observations in 30 l bioreactor, which demonstrated that the model might be useful for the development and optimization of production of cAMP in industrial scale. Based on estimated kinetic parameters, three different fed-batch modes, constant rate and intermittent (once and repeated), were adopted in order to obtain more cAMP accumulation. Furthermore, the final production of cAMP reached 11.24 g l -1 after 72 h incubation using three stages feeding strategy. In particular, the cAMP productivity (0.156 g l -1 h -1 ) was successfully improved by 22.83, 11.43 and 9.86%, respectively, compared with the modes of the batch, constant rate fed-batch and intermittent fed-batch once.

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Section: Introductionmentioning

confidence: 99%

Dynamic mathematical models of batch experiments and fed-batch cultures for cyclic adenosine monophosphate production by Arthrobacter A302

Chen

Ren

et al. 2011

World J Microbiol Biotechnol

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“…Microbial production of cAMP has attracted increasing attention because it is more sustainable and economical compared with complicated multistep chemical synthesis (Chen et al 2009;Ishiyama 1990). Microorganisms that have the ability to produce cAMP include Microbacterium sp.…”

Section: Introductionmentioning

confidence: 99%

Production of cyclic adenosine monophosphate by Arthrobacter sp. A302 using fed-batch fermentation with pH-shift control

Cao

Chen

Ren

et al. 2011

World J Microbiol Biotechnol

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The production of cyclic adenosine monophosphate (cAMP) by Arthrobacter sp. A302 was studied in a 5 L stirred tank fermentor under a range of pH values (6.5-8.0) and glucose feeding rates. In batch fermentation under a controlled pH, the optimum pH for cell growth was 7.5 with dry cell density (X) of 11.43 g L, and the optimum pH for cAMP accumulation was 7.0 with cAMP concentration of 7.41 g L. In order to achieve the high X and cAMP yield simultaneously, a pH-shift control strategy was proposed based on kinetic analysis of specific cell growth rate (μ) and specific cAMP formation rate (q ( s )). In this method, pH was controlled to 7.0 for the first 30 h of fermentation, and then subsequently shifted to 7.5 and maintained until the end of the process. Application of this approach significantly enhanced the cAMP concentration. Thereafter, cAMP production was further improved by combining the above-mentioned pH-control system and fed-batch process with glucose at a constant feeding rate of 1.0 g L(-1 )h(-1). Under optimum conditions, the final cAMP production was 10.87 g L, which is 110.0, 46.7, and 27.7% higher than that of the pH-uncontrolled, pH-controlled, and pH-shift controlled methods, respectively.

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“…The industrial production of cAMP through microorganism attracts attention of researchers for low cost and environmental friendliness. The microbes, including Microbacterium , Arthrobacter , Brevibacterium liquefacien , Corynebacterium roseoparaffineus , Corynebacterium murisepticum , were reported to be able to produce cAMP 4 , 5 .…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptomic and proteomic analysis of Arthrobacter sp. CGMCC 3584 responding to dissolved oxygen for cAMP production

Niu

Wang

Zhuang

et al. 2018

Sci Rep

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Arthrobacter sp. CGMCC 3584 is able to produce high yields of extracellular cyclic adenosine monophosphate (cAMP), which plays a vital role in the field of treatment of disease and animal food, during aerobic fermentation. However, the molecular basis of cAMP production in Arthrobacter species is rarely explored. Here, for the first time, we report the comparative transcriptomic and proteomic study of Arthrobacter cells to elucidate the higher productivity of cAMP under high oxygen supply. We finally obtained 14.1% and 19.3% of the Arthrobacter genome genes which were up-regulated and down-regulated notably, respectively, with high oxygen supply, and identified 54 differently expressed proteins. Our results revealed that high oxygen supply had two major effects on metabolism: inhibition of glycolysis, pyruvate metabolism, nitrogen metabolism, and amino acid metabolism (histidine, branched-chain amino acids and glutamate metabolism); enhancement of the tricarboxylic acid cycle and purine metabolism. We also found that regulation of adenylate cyclase and phosphodiesterase was not significant under high oxygen supply, suggesting efficient cAMP export might be important in cAMP production. These findings may contribute to further understanding of capacities of Arthrobacter species and would be highly useful in genetic regulation for desirable production.Cyclic adenosine monophosphate (cAMP) is an important compound which exists in many living cells, and participates in the regulation of physiological actions, such as cell proliferation and differentiation, hormones synthesis and secretion, membrane protein activity, nervous activity, gene expression and so on 1-3 . Based on its functions, cAMP is used in pharmaceuticals and as feed additives. The industrial production of cAMP through microorganism attracts attention of researchers for low cost and environmental friendliness. The microbes, including Microbacterium, Arthrobacter, Brevibacterium liquefacien, Corynebacterium roseoparaffineus, Corynebacterium murisepticum, were reported to be able to produce cAMP 4,5 . Our previous studies have demonstrated that a strain, Arthrobacter sp. CGMCC 3584, was capable of producing high yields of cAMP in culture media, through optimization of culture conditions and metabolic regulation on the fermentation process [6][7][8] . Arthrobacter species are among the most common aerobic culturable bacteria and exist in the nature widely, mainly in soil and in some extreme environments 9,10 . Their remarkable tolerance to various stresses contributes to the application of Arthrobacter species in contaminant degradation in complex and volatile environments 11 . Most genome and transcriptome researches of this genus focus on the genetic basis of the biodegradation and survival capacities, and available microarray data are rarely thus far. In the present study, DNA microarray-based transcriptomic analysis and two-dimensional (2D) gel electrophoresis technology

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Isolation of mutants with improved production of cAMP from Microbacterium sp. no. 205 (ATCC21376)

Cited by 15 publications

References 12 publications

Dynamic mathematical models of batch experiments and fed-batch cultures for cyclic adenosine monophosphate production by Arthrobacter A302

Dynamic mathematical models of batch experiments and fed-batch cultures for cyclic adenosine monophosphate production by Arthrobacter A302

Production of cyclic adenosine monophosphate by Arthrobacter sp. A302 using fed-batch fermentation with pH-shift control

Comparative transcriptomic and proteomic analysis of Arthrobacter sp. CGMCC 3584 responding to dissolved oxygen for cAMP production

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