Production of cyclic adenosine monophosphate by Arthrobacter sp. A302 using fed-batch fermentation with pH-shift control

Cao, Jiaming; Chen, Xiaochun; Ren, Huajing; Zhang, Jindan; Li, Lei; Chen, Yong; Xiong, Jing; Bai, Jianxin; Ying, Hanjie

doi:10.1007/s11274-011-0799-y

Cited by 5 publications

(2 citation statements)

References 27 publications

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“…This indicates that the variation of sample 99.00% had been attributed to the selected variables and only 1% of the total variance couldn't be explained by the model. A regression model have R 2 value higher than 0.9 was considered to have a very high correlation (Cao et al, 2012). Table 8.…”

Section: Multiple Regression and Analysis Of Variance (Anova)mentioning

confidence: 99%

Optimization of Bioactive Metabolites production by a Newly Isolated Marine Streptomyces sp. Using Statistical Approach

Mohamedin¹,

El‐Naggar²,

Sherief³

et al. 2015

Biotechnology

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A B S T R A C TThe newly isolated actinomycetes strain namely M19 was isolated from marine sediment and exhibited antibacterial activity against multidrug-resistant Staphylococcus epidermidis. It was identified on the basis of morphological, cultural, physiological and biochemical properties, together with 16S rRNA sequence as Streptomyces psammoticus strain M19 and sequencing product was deposited in the GenBank database under accession number KT160249. Fifteen factors including starch, KNO 3 , K 2 HPO 4 , yeast extract, NaCl, MgSO 4 , CaCO 3 , FeSO 4 , pH, temperature, agitation speed, medium volume, inoculum size, inoculum age and fermentation time had been examined for their significance on the production of bioactive metabolites using Plackett-Burman design. Out of the fifteen factors; agitation speed, yeast extract, NaCl and KNO 3 were selected due to their significant effects on the production of bioactive metabolites and the optimal levels of these variables and the effect of their interactions on bioactive metabolites production were determined by using central composite design. As a result, culture conditions and a medium of the following formula were predicted to be the optimum for production of extracellular bioactive metabolites in the culture filtrate of Streptomyces psammoticus strain M19: Starch 20 g, KNO 3 1.5 g, K 2 HPO 4 0.5 g, yeast extract 0.2 g, NaCl 0.5 g, MgSO 4 0.1 g, CaCO 3 3 g, FeSO 4 0.01 g, pH 6.5, temperature 25°C, agitation speed (125 rpm minG 1 ), medium volume 75 mL, inoculum size 2% (v/v), inoculation age 48 h and fermentation time 5 days. The antagonistic activity produced from the optimized culture conditions against multidrug-resistant Staphylococcus epidermidis, showed about 1.37 fold increase than that obtained from the un-optimized medium.

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Section: Multiple Regression and Analysis Of Variance (Anova)mentioning

confidence: 99%

Optimization of Bioactive Metabolites production by a Newly Isolated Marine Streptomyces sp. Using Statistical Approach

Mohamedin¹,

El‐Naggar²,

Sherief³

et al. 2015

Biotechnology

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“…Our previous studies have demonstrated that a strain, Arthrobacter sp. CGMCC 3584, was capable of producing high yields of cAMP in culture media, through optimization of culture conditions and metabolic regulation on the fermentation process 6 – 8 . Arthrobacter species are among the most common aerobic culturable bacteria and exist in the nature widely, mainly in soil and in some extreme environments 9 , 10 .…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptomic and proteomic analysis of Arthrobacter sp. CGMCC 3584 responding to dissolved oxygen for cAMP production

Niu

Wang

Zhuang

et al. 2018

Sci Rep

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Arthrobacter sp. CGMCC 3584 is able to produce high yields of extracellular cyclic adenosine monophosphate (cAMP), which plays a vital role in the field of treatment of disease and animal food, during aerobic fermentation. However, the molecular basis of cAMP production in Arthrobacter species is rarely explored. Here, for the first time, we report the comparative transcriptomic and proteomic study of Arthrobacter cells to elucidate the higher productivity of cAMP under high oxygen supply. We finally obtained 14.1% and 19.3% of the Arthrobacter genome genes which were up-regulated and down-regulated notably, respectively, with high oxygen supply, and identified 54 differently expressed proteins. Our results revealed that high oxygen supply had two major effects on metabolism: inhibition of glycolysis, pyruvate metabolism, nitrogen metabolism, and amino acid metabolism (histidine, branched-chain amino acids and glutamate metabolism); enhancement of the tricarboxylic acid cycle and purine metabolism. We also found that regulation of adenylate cyclase and phosphodiesterase was not significant under high oxygen supply, suggesting efficient cAMP export might be important in cAMP production. These findings may contribute to further understanding of capacities of Arthrobacter species and would be highly useful in genetic regulation for desirable production.Cyclic adenosine monophosphate (cAMP) is an important compound which exists in many living cells, and participates in the regulation of physiological actions, such as cell proliferation and differentiation, hormones synthesis and secretion, membrane protein activity, nervous activity, gene expression and so on 1-3 . Based on its functions, cAMP is used in pharmaceuticals and as feed additives. The industrial production of cAMP through microorganism attracts attention of researchers for low cost and environmental friendliness. The microbes, including Microbacterium, Arthrobacter, Brevibacterium liquefacien, Corynebacterium roseoparaffineus, Corynebacterium murisepticum, were reported to be able to produce cAMP 4,5 . Our previous studies have demonstrated that a strain, Arthrobacter sp. CGMCC 3584, was capable of producing high yields of cAMP in culture media, through optimization of culture conditions and metabolic regulation on the fermentation process [6][7][8] . Arthrobacter species are among the most common aerobic culturable bacteria and exist in the nature widely, mainly in soil and in some extreme environments 9,10 . Their remarkable tolerance to various stresses contributes to the application of Arthrobacter species in contaminant degradation in complex and volatile environments 11 . Most genome and transcriptome researches of this genus focus on the genetic basis of the biodegradation and survival capacities, and available microarray data are rarely thus far. In the present study, DNA microarray-based transcriptomic analysis and two-dimensional (2D) gel electrophoresis technology

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Enhanced Adenosine Production by Bacillus subtilis at Condition with Comprehensively Controlled Dissolved Oxygen and pH During Fermentation

Liu¹,

He²,

Xu³

et al. 2015

Lecture Notes in Electrical Engineering

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Production of cyclic adenosine monophosphate by Arthrobacter sp. A302 using fed-batch fermentation with pH-shift control

Cited by 5 publications

References 27 publications

Optimization of Bioactive Metabolites production by a Newly Isolated Marine Streptomyces sp. Using Statistical Approach

Optimization of Bioactive Metabolites production by a Newly Isolated Marine Streptomyces sp. Using Statistical Approach

Comparative transcriptomic and proteomic analysis of Arthrobacter sp. CGMCC 3584 responding to dissolved oxygen for cAMP production

Enhanced Adenosine Production by Bacillus subtilis at Condition with Comprehensively Controlled Dissolved Oxygen and pH During Fermentation

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