Isolation of multipotent stem cells in human periodontal ligament using stage-specific embryonic antigen-4

Notch Signaling Is Involved in Neurogenic Commitment of Human Periodontal Ligament-Derived Mesenchymal Stem Cells

“…Thus, the change of intracellular calcium resulted from NMDA or DPH treatment in the present study, implying the expression of particular receptors and their function. Similar to the present study, neuronal differentiation of hPDLSCs has previously been reported [5,23].…”

Expression Profile of the Stem Cell Markers in Human Hertwig’s Epithelial Root Sheath/Epithelial Rests of Malassez Cells

“…The transcription factors Oct-4 (Rosner et al, 1990;Scholer et al, 1990) and Nanog (Chambers et al, 2003;Mitsui et al, 2003) are highly expressed in EmSCs and play a central role in the regulation of their pluripotency and self-renewal. Additionally, SSEA-4, a positive marker of human embryonic stem cells (Thomson et al, 1995; that has recently been used for isolating primitive stem cells from bone marrow and periodontium (Gang et al, 2007;Kawanabe et al, 2010), was also expressed in the HERS/ERM cells. The expression of these genes suggests that HERS/ERM cells might contain more primitive stem cell characteristics.…”

Is There a Role for Neural Crest Stem Cells in Periodontal Regeneration?

“…PDLSC were first isolated from the PDL tissue of extracted human third molar teeth [49]. PDLSC expressed MSC-related cell surface markers, CD10, CD13, CD26, CD29, CD44, CD71, CD73, CD90, CD105, CD106, CD146, CD166, CD349, STRO-1, STRO-3, and TNAP/MSCA-1 [50][51][52], as well as neural crest cell-related marker genes Snail, Slug, Twist, SOX9, SOX10, Nestin, p75NTR, CD49d, and Tuj1 [53,54]. PDLSC cultured in osteogenic medium can form mineralized nodules and demonstrate an increase in bonerelated gene expression [49] and in vivo transplantation of PDLSC into calvarial defects revealed the new bone formation within the defects [55,56], suggesting that PDLSC can differentiate into osteoblasts.…”