Isolation of multidrug resistant coliforms and their bacteriophages from swine slurry

Abstract

Slurry management is a contemporary agricultural problem. Although liquid manure is a valuable fertiliser, it may pose a microbiological threat due to spreading of bacteria resistant to various antibiotics. Fermentation has been proposed as a solution to this problem, although it does not fully eliminate the antibiotic-resistant strains. The presence of gram-negative rods resistant to antimicrobials may cause an uncontrolled transmission of resistance factors and genes in the natural environment. Therefore, a biocontrol system is necessary to prevent this phenomenon. The aim of this study was to detect multidrug-resistant (MDR) bacteria and their lytic bacteriophages in raw swine slurry. For that purpose, MDR bacteria from the family Enterobacteriaceae were isolated and tested with bacteriophages using a modified detection method. The study suggests that the bacteriophages present in the slurry are active against MDR bacteria and possibly could be used as a biocontrol agent for reduction of these microorganisms in fertilisers of animal origin. In the scope of the results obtained, a novel approach for the evaluation of slurry based on the preselection method is proposed. Moreover, the study reports the first case of isolation of a bacteriophage active against Providencia alcalifaciens.

DRUG RESISTANCE OF Pseudomonas spp. STRAINS ISOLATED FROM WATER RESERVOIRS AND SEWAGE TREATMENT PLANTS IN SZCZECIN

“…Such microorganisms can be found e.g. in slurry (Augustyniak et al 2018). Antibiotics and microorganisms can be transferred into the ground, as well as into groundwater and other water reservoirs.…”

Potential of using bacteriophages and nanomaterials for eradicating bacterial diseases in animals

“…Ponadto, mimo że stosowanie bakteriofagów zwłaszcza podczas prób klinicznych u ludzi wciąż budzi wątpliwości, firmy farmaceutyczne mogą chętniej inwestować w terapię fagową do zastosowań weterynaryjnych (23). Z jednej strony, wykazują znaczący potencjał antybakteryjny i jako pasażowalne jednostki bakteriolityczne są wykorzy-Perspektywiczne możliwości wykorzystania kombinacji działania bakteriofagów i nanomateriałów w zwalczaniu chorób bakteryjnych u zwierząt stywane w jedno-lub wielofagowej (koktajle fagowe) terapii chorób bakteryjnych, w tym wywoływanych przez szczepy wielolekooporne, z drugiej natomiastmożna je stosować jako nośniki DNA, białek lub leków (1,5,11). Duże znaczenie zyskują też fagowe białka lityczne (endolizyny i hydrolazy) efektywnie degradujące peptydoglikan bakterii Gram-dodatnich, których potencjał wykazano w leczeniu chorób bakteryjnych na modelach zwierzęcych (38).…”

Biofilms in the gravity sewer interfaces: making a friend from a foe

“…Studies also show that bacteriophages pose reducing activity against multispecies biofilms (Lehman and Donlan 2015;Gutiérrez et al 2015). Unfortunately, due to the complexity of environmental conditions in sewer systems and the constant co-evolution of bacteriophages and their hosts, phage application in wastewater needs further investigation (Jassim et al 2016;Jurczak-Kurek et al 2016;Grygorcewicz et al 2017;Augustyniak et al 2018;Guerrero et al 2020).…”

“…Hypothetically, the purpose of such reduction could be breaking metabolic chains through the elimination of taxa responsible for a reaction. Other applications could be associated with bacteriophages for the eradication of antibiotic-resistant pathogens and water-contaminating microorganisms (Amin et al 2014;Mattila et al 2015;Augustyniak et al 2018). Eventually, these viruses could specifically reduce the number (or change the populations) of acid-producing bacteria leading to higher sustainability of cementitious composite.…”