Isolation of microsatellite markers in the digenetic trematode <i>Schistosoma mansoni</i> from Guadeloupe island

Durand, Patrick; Sire, C.; Thèron, André

doi:10.1046/j.1365-294x.2000.00939-4.x

Cited by 66 publications

(64 citation statements)

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“…As genetic differentiation within infrapopulations in rodents has been found (Sire et al 2001), DNA microsatellite markers could be used to investigate the genetic structuring of natural populations (Durand et al 2000. Finally, the results presented here support the need for further investigations into the possible selective pressures acting on both populations.…”

Section: Discussionsupporting

confidence: 61%

A comparative parasitologic study on Biomphalaria glabrata snail and C3H/He mice infected with human and murine isolates of Schistosoma mansoni derived from Sumidouro, Rio de Janeiro, Brazil

Freire

Rodrigues-Silva

Machado-Silva

et al. 2003

Mem. Inst. Oswaldo Cruz

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Section: Discussionsupporting

confidence: 61%

A comparative parasitologic study on Biomphalaria glabrata snail and C3H/He mice infected with human and murine isolates of Schistosoma mansoni derived from Sumidouro, Rio de Janeiro, Brazil

Freire

Rodrigues-Silva

Machado-Silva

et al. 2003

Mem. Inst. Oswaldo Cruz

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“…These sequences are distributed in the S. cerevisiae genome ( Table 2). PCR amplification was performed according to the protocol described by Durand et al (8).…”

Section: Methodsmentioning

confidence: 99%

Typing of Saccharomyces cerevisiae Clinical Strains by Using Microsatellite Sequence Polymorphism

et al. 2005

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“…In short, individual S. mansoni parasites were genotyped using nine putatively neutral microsatellite markers (L46951, CA11-1, S9-1, SMD11, SMD25, SMD28, SMD43, SMD89, SMDA28; Durand et al, 2000;Blair et al, 2001;Curtis et al, 2001). PCR products were analyzed using an ABI 3130 Genetic Analyser (Applied Biosystems, Foster City, CA, USA) and GeneScan 500 LIZ (Applied Biosystems) as size standard.…”

Section: Molecular Analysismentioning

confidence: 99%

Inbreeding within human Schistosoma mansoni: do host-specific factors shape the genetic composition of parasite populations?

et al. 2014

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The size, structure and distribution of host populations are key determinants of the genetic composition of parasite populations. Despite the evolutionary and epidemiological merits, there has been little consideration of how host heterogeneities affect the evolutionary trajectories of parasite populations. We assessed the genetic composition of natural populations of the parasite Schistosoma mansoni in northern Senegal. A total of 1346 parasites were collected from 14 snail and 57 human hosts within three villages and individually genotyped using nine microsatellite markers. Human host demographic parameters (age, gender and village of residence) and co-infection with Schistosoma haematobium were documented, and S. mansoni infection intensities were quantified. F-statistics and clustering analyses revealed a random distribution (panmixia) of parasite genetic variation among villages and hosts, confirming the concept of human hosts as 'genetic mixing bowls' for schistosomes. Host gender and village of residence did not show any association with parasite genetics. Host age, however, was significantly correlated with parasite inbreeding and heterozygosity, with children being more infected by related parasites than adults. The patterns may be explained by (1) genotype-dependent 'concomitant immunity' that leads to selective recruitment of genetically unrelated worms with host age, and/or (2) the 'genetic mixing bowl' hypothesis, where older hosts have been exposed to a wider variety of parasite strains than children. The present study suggests that host-specific factors may shape the genetic composition of schistosome populations, revealing important insights into host-parasite interactions within a natural system.

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Isolation of microsatellite markers in the digenetic trematode Schistosoma mansoni from Guadeloupe island

Cited by 66 publications

References 3 publications

A comparative parasitologic study on Biomphalaria glabrata snail and C3H/He mice infected with human and murine isolates of Schistosoma mansoni derived from Sumidouro, Rio de Janeiro, Brazil

A comparative parasitologic study on Biomphalaria glabrata snail and C3H/He mice infected with human and murine isolates of Schistosoma mansoni derived from Sumidouro, Rio de Janeiro, Brazil

Typing of Saccharomyces cerevisiae Clinical Strains by Using Microsatellite Sequence Polymorphism

Inbreeding within human Schistosoma mansoni: do host-specific factors shape the genetic composition of parasite populations?

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