A comparative parasitologic study on Biomphalaria glabrata snail and C3H/He mice infected with human and murine isolates of Schistosoma mansoni derived from Sumidouro, Rio de Janeiro, Brazil

Abstract

Experiments were carried out to analyze the biological characteristics of two sympatric isolates of

The outcome of acute schistosomiasis infection in adult mice with postnatal exposure to maternal malnutrition

“…Procedures for experimental infections have been described previously (Freire et al 2003). At two months old, mouse pups were divided in six groups, according to diet: uninfected C, uninfected PR, uninfected CR, infected C (IC), infected PR (IPR) and infected CR (ICR).…”

“…Infected mice were euthanized by cervical dislocation at 24 h after the last faecal examination. Parasite burden (infectivity) was calculated as the percentage of cercariae recovered from the portal system and mesenteric veins that had matured into adult worms (Freire et al 2003).…”

“…Segments were opened, washed and crushed between two glass slides to obtain a thin preparation. The percentage of immature, mature and dead S. mansoni eggs was determined by conventional light microscopy (Freire et al 2003).…”

Biological implications of the phenotypic plasticity in the Schistosoma mansoni - Nectomys squamipes model

“…On day 63 post-infection, all rats were killed by cervical dislocation. Adult worms were recovered from the portal system and mesenteric veins, counted in a stereomicroscope and infection rate was assessed as a percentage of cercariae that had matured into adult worms 12 .…”

Assessment of Akodon cursor (Rodentia, Sigmodontinae) as permissive host to Schistosoma mansoni infection: morphology of adult worms

“…The S. mansoni life cycle is maintained routinely in Biomphalaria glabrata snails and mice at the Laboratório de Malacologia (Instituto Oswaldo Cruz, Brasil) and prepared by exposing infected snails to light for 2 hrs to induce shedding of parasites (Freire et al 2003). Each mouse was exposed to approximately 150 S. mansoni cercariae (BH strain, Brazil) by subcutaneous route, as previously reported (Machado e Silva et al 1991).…”