Isolation of major phenolics from<i>Launaea spinosa</i>and their protective effect on HepG2 cells damaged with<i>t</i>-BHP

Abdallah, Hossam M.; Farag, Mohamed A.; Kim, Da Hye; Kang, Kyungsu; Pan, Cheol‐Ho; Abdel‐Sattar, Essam

doi:10.3109/13880209.2015.1052885

Cited by 19 publications

(13 citation statements)

References 19 publications

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“…These results indicated that polyphenol components of the HB extracts have an effect on the cell viability in HepG2 cells induced by t-BHP. This finding is supported by another study where phenolic compounds displayed protective effects against oxidative stress, leading to the maintenance of a normal redox state of the cell [ 22 ]. HBK and HBC showed increased cell viabilities in a dose-dependent manner, although 30 µg/mL of HBK did not show a significant increase in cell viabilities.…”

Section: Discussionsupporting

confidence: 61%

Evaluation of in vitro anti-oxidant and anti-inflammatory activities of Korean and Chinese Lonicera caerulea

et al. 2018

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BACKGROUND/OBJECTIVESThe honeysuckle berry (HB) contains ascorbic acid and phenolic components, especially anthocyanins, flavonoids, and low-molecular-weight phenolic acids. In order to examine the potential of HB as a hepatoprotective medicinal food, we evaluated the in vitro anti-oxidant and anti-inflammatory activities of Korean HB (HBK) and Chinese HB (HBC).MATERIALS/METHODSAntioxidant and anti-inflammatory effects of the extracts were examined in HepG2 and RAW 264.7 cells, respectively. The anti-oxidant capacity was determined by DPPH, SOD, CAT, and ARE luciferase activities. The production of nitric oxide (NO) as an inflammatory marker was also evaluated. The Nrf2-mediated mRNA levels of heme oxygenase-1 (HO-1), NAD(P)H dehydrogenase [quinone] 1 (Nqo1), and glutamate-cysteine ligase catalytic subunit (Gclc) were measured. The concentrations of HB extracts used were 3, 10, 30, 100, and 300 µg/mL.RESULTSThe radical scavenging activity of all HB extracts increased in a concentration-dependent manner (P < 0.01 or P < 0.05). SOD (P < 0.05) and CAT (P < 0.01) activities were increased by treatment with 300 µg/mL of each HB extract, when compared to those in the control. NO production was observed in cells pretreated with 100 or 300 µg/mL of HBC and HBK (P < 0.01). Treatment with 300 µg/mL of HBC significantly increased Nqo1 (P < 0.01) and Gclc (P < 0.05) mRNA levels compared to those in the control. Treatment with 300 µg/mL of HBK (P < 0.05) and HBC (P < 0.01) also significantly increased the HO-1 mRNA level compared to that in the control.CONCLUSIONSThus, the Korean and Chinese HBs were found to possess favorable in vitro anti-oxidant and anti-inflammatory activities. Nrf2 and its related anti-oxidant genes were associated with both anti-oxidant and anti-inflammatory activities in HB-treated cells. Further studies are needed to confirm these in vivo effects.

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Section: Discussionsupporting

confidence: 61%

Evaluation of in vitro anti-oxidant and anti-inflammatory activities of Korean and Chinese Lonicera caerulea

et al. 2018

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“…The exception was found to be natural resistance of this fungus to these extracts (Okwori et al, 2008). The antifungal activities and cytoprotective effect of medicinal plants are attributed to the presence of flavonoids, tannins and phenolic compounds (Abdallah et al, 2015). The traditional healers make use of water primarily as a solvent, but our study showed that, different extracts of both plants and latex were much better and powerful.…”

Section: Antimicrobial Activitymentioning

confidence: 60%

“…Many of Launaea species (family Asteracea) are used in folk medicine as hepatoprotective, bitter stomachic, for treating skin diseases, and reported to have antitumor, insecticidal and cytoprotective activities (El-Bassuony andAbdel-Hamid, 2006 andKhan et al, 2012). This genus is rich in different secondary metabolites, such as terpenoids (Abdel Fattah et al, 1990), phenolic compounds (Abdallah et al, 2015) and coumarins (El-Bassuony and Abdel-Hamid, 2006). Abdallah et al (2015) reported that some of Launaea species are used traditionally to treat liver oxidative stress.…”

Section: Osmoregulation and Antimicrobial Activity Of Two Egyptian Trmentioning

confidence: 99%

“…This genus is rich in different secondary metabolites, such as terpenoids (Abdel Fattah et al, 1990), phenolic compounds (Abdallah et al, 2015) and coumarins (El-Bassuony and Abdel-Hamid, 2006). Abdallah et al (2015) reported that some of Launaea species are used traditionally to treat liver oxidative stress. The potential cytoprotective activity of phenolic compounds isolated from L. spinosa was assessed against oxidative stress induced by tert-butyl hydroperoxide (t-BHP) in HepG2 cells in order to relate in vitro antioxidant activity with cytoprotective effect.…”

Section: Osmoregulation and Antimicrobial Activity Of Two Egyptian Trmentioning

confidence: 99%

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Osmoregulation and Antimicrobial Activity of Two Egyptian True Xerophytes; Launaea Spinosa (Forssk.) and Leptadenia Pyrotechnica (Forssk.)

Khidr

Ebad

El-khawaga

2017

Egyptian Journal of Desert Research

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n the present work, some of the adaptive responses against dehydrative factors were evaluated in two true xerophytic plants, for their medicinal importance; [Launaea spinosa (Forssk.) Sch. and Leptadenia pyrotechnica (Forssk.) Decne.] growing naturally (wild plants) at Wadi Hagul, Eastern Desert of Egypt during both wet and dry seasons. The results reveled that Launaea spinosa achieved the highest significant ash concentration during the dry season and this was mainly due to accumulation of Na + , K + , Cland SO 4 2-, meanwhile the opposite was found in Leptadenia pyrotechnica, which accumulated Ca 2+ , Mg 2+ and P 3+ during the wet season. The highest significant concentrations of total carbohydrates, insoluble sugars as well as phytohormones were recorded during the wet season, while organic acids, free thiols, total fibers, and soluble sugars were accumulated, in both species, during the dry season. From the results it is clear that, ethyl acetate extracts for both species possessed the highest antimicrobial activity against all tested fungal and bacterial strains. The obtained results showed that the studied species have different mechanisms to adapt and tolerate the various environmental conditions.

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“…Cells. To evaluate hepatotoxicity induced by t-BHP, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were measured using the ALT and AST kit (Biovision, CA) as previously described (Abdallah et al 2016). HepG2 cells were seeded into 24-well plates (2.5 3 10 5 cells per well) and incubated for 24 h. The cells were treated with various concentrations of AFR, AFP, quercetin and DMSO for 24 h, and then with 200 lM t-BHP for 3 h. The cells were then washed with DPBS and harvested by centrifugation at 2,870 3 g for 5 min at 4C.…”

Section: Measurement Of Alt and Ast Activities In Hepg2mentioning

confidence: 99%

The Protective Effect of Antioxidant Enriched Fractions from Colored Potatoes Against Hepatotoxic Oxidative Stress in Cultured Hepatocytes and Mice

Kim

et al. 2016

Journal of Food Biochemistry

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Herein, we prepared standardized antioxidant‐enriched fractions from red (AFR) and purple (AFP) colored potatoes and evaluated their protective effects against hepatotoxicity induced by oxidative stress. The major compound contained in both AFR and AFP was 5‐caffeoylquinic acid. Experiments in vitro showed that AFR and AFP protected against HepG2 cell death induced by tert‐butyl hydroperoxide (t‐BHP) as determined by cell viability assay. AFR and AFP recovered alanine aminotransferase (ALT) and aspartate aminotransferase (AST) enzyme activities, which increased by t‐BHP treatment in HepG2 cells. AFR and AFP also inhibited the cleavage of PARP and caspase‐3 in HepG2 cells. Moreover, in vivo experiments in mice showed that AFR and AFP protected against t‐BHP‐induced hepatotoxicity and recovered serum ALT and AST enzyme activities. AFR and AFP also rescued serum antioxidant enzyme activities, catalase and glutathione peroxidase, which were disturbed by t‐BHP treatment. Our data clearly demonstrate that AFR and AFP protect against hepatic damage induced by oxidative stress. Practical Applications Colored potatoes contain plentiful amounts of antioxidant compounds that can be developed into dietary supplements for the improvement of human health. We prepared antioxidant‐enriched fractions from red and purple colored potatoes (AFR and AFP, respectively) to be used as hepatoprotective agents. Based on chemical profiling analysis, we found that 5‐caffeoylquinic acid can serve as a marker for the standardization of AFR and AFP as functional food materials. AFR and AFP strongly protected against oxidative stress and injury both in cultured hepatocytes and mice. AFR and AFP prevented the cell death of hepatocytes, and they also rescued hepatic damage markers ALT and AST both in cell and animal experiments. Generally, AFP showed higher hepatoprotective activity than AFR. Taken together, AFR and AFP may be promising candidates as functional foods for improving human liver function.

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Isolation of major phenolics fromLaunaea spinosaand their protective effect on HepG2 cells damaged witht-BHP

Abstract: The major phenolic compounds isolated from L. spinosa displayed a significant cytoprotective effect against oxidative stress, leading to maintenance of the normal redox status of the cell.

Cited by 19 publications

References 19 publications

Evaluation of in vitro anti-oxidant and anti-inflammatory activities of Korean and Chinese Lonicera caerulea

Evaluation of in vitro anti-oxidant and anti-inflammatory activities of Korean and Chinese Lonicera caerulea

Osmoregulation and Antimicrobial Activity of Two Egyptian True Xerophytes; Launaea Spinosa (Forssk.) and Leptadenia Pyrotechnica (Forssk.)

The Protective Effect of Antioxidant Enriched Fractions from Colored Potatoes Against Hepatotoxic Oxidative Stress in Cultured Hepatocytes and Mice

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