The Protective Effect of Antioxidant Enriched Fractions from Colored Potatoes Against Hepatotoxic Oxidative Stress in Cultured Hepatocytes and Mice

Kim, Da Hye; Kim, Myungsuk; Oh, Sang-Bin; Lee, Kyung Mi; Kim, Sang Min; Nho, Chu Won; Yoon, Won Byong; Kang, Kyungsu; Pan, Cheol‐Ho

doi:10.1111/jfbc.12315

Cited by 5 publications

(5 citation statements)

References 29 publications

(45 reference statements)

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“…The treatments have been described previously [22]. All animals were randomly divided into five groups (n = 7 in each group) and orally administered (p.o.…”

Section: Animal Treatmentmentioning

confidence: 99%

Probiotics Alleviate Oxidative Stress in H2O2-Exposed Hepatocytes and t-BHP-Induced C57BL/6 Mice

Lee¹,

Kang²

2022

Microorganisms

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Antioxidants protect against oxidative stress that can damage proteins, the cellular immune system, and DNA. In recent studies, probiotics have been shown to impart a microbial balance to the gastrointestinal tract, demonstrating significant antioxidant capacity. In this study, the probiotic properties and antioxidant mechanism of probiotics were evaluated in HepG2 cells and in an animal model. The characteristics of Lactococcus lactis MG5125, Bifidobacterium bifidum MG731, and Bifidobacterium animalis subsp. lactis MG741, which were used as lactic acid bacteria in this study, were analyzed. The results revealed the safety and stability of these probiotics in the gastrointestinal tract because they did not cause hemolysis and had excellent intestinal adhesion (75–84%). In HepG2 cells, the three probiotics alleviated H2O2-induced oxidative stress by mediating lipid peroxidation and glutathione levels and upregulating antioxidant enzymes, including catalase, superoxide dismutase, and glutathione peroxidase. In the tBHP-induced mouse model, administration of the three probiotics reduced hepatic aspartate transaminase, alanine transaminase, and lipid peroxidation levels. In conclusion, Lc. lactis MG5125, B. bifidum MG731, and B. lactis MG741 showed considerable antioxidant activity both in vitro and in vivo.

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“…The treatments have been described previously [22]. All animals were randomly divided into five groups (n = 7 in each group) and orally administered (p.o.…”

Section: Animal Treatmentmentioning

confidence: 99%

Probiotics Alleviate Oxidative Stress in H2O2-Exposed Hepatocytes and t-BHP-Induced C57BL/6 Mice

Lee¹,

Kang²

2022

Microorganisms

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“…2019 ). Consumption of these anthocyanins can have health benefits ( Kim et al . 2017 ); thus, this gene warrants further study with reference to human health and exemplifies the potential application of this positive selection analysis.…”

Section: Discussionmentioning

confidence: 99%

Extensive crop–wild hybridization during Brassica evolution and selection during the domestication and diversification of Brassica crops

et al. 2023

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Adaptive genetic diversity in crop wild relatives (CWRs) can be exploited to develop improved crops with higher yield and resilience if phylogenetic relationships between crops and their CWRs are resolved. This further allows accurate quantification of genome-wide introgression and determination of regions of the genome under selection. Using broad sampling of CWRs and whole genome sequencing we further demonstrate the relationships among two economically valuable and morphologically diverse Brassica crop species, their CWRs and their putative wild progenitors. Complex genetic relationships and extensive genomic introgression between CWRs and Brassica crops were revealed. Some wild B. oleracea populations have admixed feral origins, some domesticated taxa in both crop species are of hybrid origin, while wild B. rapa is genetically indistinct from turnips. The extensive genomic introgression we reveal could result in false identification of selection signatures during domestication using traditional comparative approaches used previously, therefore we adopted a single population approach to study selection during domestication. We used this to explore examples of parallel phenotypic selection in the two crop groups and highlight promising candidate genes for future investigation. Our analysis defines the complex genetic relationships between Brassica crops and their diverse CWRs, revealing extensive cross-species gene flow with implications for both crop domestication and evolutionary diversification more generally.

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“…The extracts were administered orally for 14 days with a feeding needle catheter, and the mice received an intraperitoneal (i.p.) injection of t-BHP (0.5 mmol/kg) after 3 h from the last feed [20]. Following euthanasia, the liver tissues were dissected and stored at −80 • C for biochemical parameter analysis.…”

Section: Animal Treatmentsmentioning

confidence: 99%

Antioxidant Effect via Bioconversion of Isoflavonoid in Astragalus membranaceus Fermented by Lactiplantibacillus plantarum MG5276 In Vitro and In Vivo

Lee¹,

Park²,

Kang³

2022

Fermentation

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In this study, the antioxidant mechanism of Astragalus membranaceus fermented by Lactiplantibacillusplantarum MG5276 (MG5276F-AM) was evaluated in HepG2 cells and in an animal model. HPLC analysis was performed to confirm the bioconversion of the bioactive compounds in A. membranaceus by fermentation. Calycosin and formononetin, which were not detected before fermentation (NF-AM), were detected after fermentation (MG5276F-AM), and its glycoside was not observed in MG5276F-AM. In HepG2 cells, MG5276F-AM alleviated H2O2-induced oxidative stress by mediating lipid peroxidation and glutathione levels, and upregulated antioxidant enzymes including catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx). In the tBHP-injected mouse model, administration of MG5276F-AM reduced hepatic aspartate transaminase, alanine transaminase, and lipid peroxidation. MG5276F-AM also modulated antioxidant enzymes as well as HepG2 cells. Thus, fermentation of A. membranaceus with L. plantarum MG5276 elevated the isoflavonoid aglycone by hydrolysis of its glycosides, and this bioconversion enhanced antioxidant activity both in vitro and in vivo.

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The Protective Effect of Antioxidant Enriched Fractions from Colored Potatoes Against Hepatotoxic Oxidative Stress in Cultured Hepatocytes and Mice

Cited by 5 publications

References 29 publications

Probiotics Alleviate Oxidative Stress in H2O2-Exposed Hepatocytes and t-BHP-Induced C57BL/6 Mice

Probiotics Alleviate Oxidative Stress in H2O2-Exposed Hepatocytes and t-BHP-Induced C57BL/6 Mice

Extensive crop–wild hybridization during Brassica evolution and selection during the domestication and diversification of Brassica crops

Antioxidant Effect via Bioconversion of Isoflavonoid in Astragalus membranaceus Fermented by Lactiplantibacillus plantarum MG5276 In Vitro and In Vivo

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