Isolation of Lightning-Competent Soil Bacteria

Cérémonie, Hélène; Buret, François; Simonet, Pascal; Vogel, Timothy M.

doi:10.1128/aem.70.10.6342-6346.2004

Cited by 45 publications

(30 citation statements)

References 31 publications

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“…In two lightning-induced transformation experiments at 20°C, strains N3 and Ee2.2 achieved their highest transformation frequencies, 10 Ϫ4 and 10 Ϫ5 , respectively, in comparison to the transformation frequency of Ͻ10 Ϫ9 displayed by both control strains E. coli DH10B and P. fluorescens C7R12. The electroporation efficiencies for the uptake of pBHC in the presence of 0.5 M sucrose by strains N3 and Ee2.2 ranged from 10 4 to 10 5 CFU/g of plasmid DNA (51). These data suggest that antibiotic resistance gene acquisition by S. maltophilia strains can occur through lightning-induced transformation in the environment, and subsequently, when these strains gain entry into the clinical setting, they can retain the antibiotic resistance phenotype (31,51).…”

Section: Molecular Ecology and Stenotrophomonas Infection Gene Transfmentioning

confidence: 88%

“…The electroporation efficiencies for the uptake of pBHC in the presence of 0.5 M sucrose by strains N3 and Ee2.2 ranged from 10 4 to 10 5 CFU/g of plasmid DNA (51). These data suggest that antibiotic resistance gene acquisition by S. maltophilia strains can occur through lightning-induced transformation in the environment, and subsequently, when these strains gain entry into the clinical setting, they can retain the antibiotic resistance phenotype (31,51).…”

Section: Molecular Ecology and Stenotrophomonas Infection Gene Transfmentioning

confidence: 88%

“…Lightning has also been implicated in DNA transfer between bacterial cells (51). Two Pseudomonas sp.…”

Section: Molecular Ecology and Stenotrophomonas Infection Gene Transfmentioning

confidence: 99%

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Stenotrophomonas maltophilia: an Emerging Global Opportunistic Pathogen

2012

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SUMMARY Stenotrophomonas maltophilia is an emerging multidrug-resistant global opportunistic pathogen. The increasing incidence of nosocomial and community-acquired S. maltophilia infections is of particular concern for immunocompromised individuals, as this bacterial pathogen is associated with a significant fatality/case ratio. S. maltophilia is an environmental bacterium found in aqueous habitats, including plant rhizospheres, animals, foods, and water sources. Infections of S. maltophilia can occur in a range of organs and tissues; the organism is commonly found in respiratory tract infections. This review summarizes the current literature and presents S. maltophilia as an organism with various molecular mechanisms used for colonization and infection. S. maltophilia can be recovered from polymicrobial infections, most notably from the respiratory tract of cystic fibrosis patients, as a cocolonizer with Pseudomonas aeruginosa . Recent evidence of cell-cell communication between these pathogens has implications for the development of novel pharmacological therapies. Animal models of S. maltophilia infection have provided useful information about the type of host immune response induced by this opportunistic pathogen. Current and emerging treatments for patients infected with S. maltophilia are discussed.

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Section: Molecular Ecology and Stenotrophomonas Infection Gene Transfmentioning

confidence: 88%

Section: Molecular Ecology and Stenotrophomonas Infection Gene Transfmentioning

confidence: 88%

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Stenotrophomonas maltophilia: an Emerging Global Opportunistic Pathogen

2012

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“…The absence of homologous sequences or origins of replication were identified as major barriers to HGT by transformation (Thomas and Nielsen, 2005). Furthermore, spontaneous transformation or transformation by lightning (Cérémonie et al, 2004(Cérémonie et al, , 2006 was also described for some bacteria which do not possess genes involved in natural competence.…”

Section: Natural Transformationmentioning

confidence: 99%

Safety Assessment of Transgenic Organisms, Volume 4

2010

Harmonisation of Regulatory Oversight in Biotechnology

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io s a fe t y nv ir on me nt ag ri cu lt ur e bi os af et y g r ic u lt u r e e n vi r o n m e n t gr ic ul tu re bi os af et y en vi ro nm en t n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en v io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm e g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f gr ic ul tu re bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t a n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f gr ic ul tu re bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t a n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y e g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et...

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“…This can occur through natural transformation (sometimes electro-transformation due to atmospheric lighting) (87,88), but usually plasmid transit from one bacterium to another occurs through conjugation. This phenomenon includes different mechanisms of DNA delivery that require a physical contact between a donor and a recipient, often facilitated by specific pili which bridge and bring together mating partners.…”

Section: Conjugation and Transfer Functionsmentioning

confidence: 99%

Mining Environmental Plasmids for Synthetic Biology Parts and Devices

2015

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The scientific and technical ambition of contemporary synthetic biology is the engineering of biological objects with a degree of predictability comparable to those made through electric and industrial manufacturing. To this end, biological parts with given specifications are sequence-edited, standardized, and combined into devices, which are assembled into complete systems. This goal, however, faces the customary context dependency of biological ingredients and their amenability to mutation. Biological orthogonality (i.e., the ability to run a function in a fashion minimally influenced by the host) is thus a desirable trait in any deeply engineered construct. Promiscuous conjugative plasmids found in environmental bacteria have evolved precisely to autonomously deploy their encoded activities in a variety of hosts, and thus they become excellent sources of basic building blocks for genetic and metabolic circuits. In this article we review a number of such reusable functions that originated in environmental plasmids and keep their properties and functional parameters in a variety of hosts. The properties encoded in the corresponding sequences include inter alia origins of replication, DNA transfer machineries, toxin-antitoxin systems, antibiotic selection markers, site-specific recombinases, effector-dependent transcriptional regulators (with their cognate promoters), and metabolic genes and operons. Several of these sequences have been standardized as BioBricks and/or as components of the SEVA (Standard European Vector Architecture) collection. Such formatting facilitates their physical composability, which is aimed at designing and deploying complex genetic constructs with new-to-nature properties.

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Isolation of Lightning-Competent Soil Bacteria

Cited by 45 publications

References 31 publications

Stenotrophomonas maltophilia: an Emerging Global Opportunistic Pathogen

Stenotrophomonas maltophilia: an Emerging Global Opportunistic Pathogen

Safety Assessment of Transgenic Organisms, Volume 4

Mining Environmental Plasmids for Synthetic Biology Parts and Devices

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