2006
DOI: 10.2174/092986606778256207
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Isolation of Influenza Virus A Hemagglutinin C-Terminal Domain by Hemagglutinin Proteolysis in Octylglucoside Micelles

Abstract: A method of isolation of hydrophobic membrane-bound C-terminal domain of influenza virus A hemagglutinin (HA) is suggested. The method is based on the insertion of HA into octylglucoside micelles followed by pepsin or thermolysin hydrolysis. Subsequent treatment of proteolytic digests with chloroform-hexafluoroisopropanol mixture resulted in the extraction of a few hydrophobic peptides into organic phase. Mass-spectrometry (MALDI-TOF) analysis revealed that the peptides with ion masses corresponding to the anc… Show more

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“…1 Th2 cytokines and eosinophilic inflammation of the airway play important roles in the pathogenesis of bronchial asthma. 24 The effectiveness of ICS for asthma control is widely recognized; inhaled corticosteroids (ICS) are now used as first-line therapy for airway eosinophilic inflammation and hyperresponsiveness in asthmatic patients. 5 However, adequate dosing with ICS according to disease activity is important for achieving and maintaining ideal disease control in asthmatic patients.…”
Section: Introductionmentioning
confidence: 99%
“…1 Th2 cytokines and eosinophilic inflammation of the airway play important roles in the pathogenesis of bronchial asthma. 24 The effectiveness of ICS for asthma control is widely recognized; inhaled corticosteroids (ICS) are now used as first-line therapy for airway eosinophilic inflammation and hyperresponsiveness in asthmatic patients. 5 However, adequate dosing with ICS according to disease activity is important for achieving and maintaining ideal disease control in asthmatic patients.…”
Section: Introductionmentioning
confidence: 99%