Isolation of infective Zika virus from urine and saliva of patients in Brazil

Bonaldo, Myrna C.; Ribeiro, Ieda Pereira; Lima, Noemia S.; Santos, Alexandre Araújo Cunha dos; Menezes, Lidiane Souza Raphael; Cruz, Stephanie Oliveira Diaz da; Mello, Iasmim S. da; Furtado, Nathália Dias; Moura, Elaine E. de; Damasceno, Luana; Silva, Keli A. B. da; Castro, Marcia G.; Gerber, Alexandra Lehmkuhl; Almeida, Luiz G. P. da; Lourenço‐de‐Oliveira, Ricardo; Vasconcelos, Ana Tereza Ribeiro de; Brasil, Patrícia

doi:10.1101/045443

Cited by 36 publications

(44 citation statements)

References 43 publications

(43 reference statements)

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“…In order to further clarify the relationship between target proteins and the pathways, KEGG enrichment was performed 37 term pathways were found according to P < 0.05 by STRING, the top 20 significantly enriched pathways were mainly involved in Influenza A, Neuroactive ligand-receptor interaction, Osteoclast differentiation Serotonergic synapse, Necroptosis, Regulation of lipolysis in adipocytes, Arachidonic acid metabolism, Kaposi's sarcoma-associated herpesvirus infection, Leishmaniasis, Cytokine-cytokine receptor interaction, NF-kappa B signaling pathway, etc. (Fig.5c)Discussion and ConclusionAt present study and clinical diagnosis reported, Zika fever is usually asymptomatic or mild in adults by ZIKV infection, but ZIKV can quickly resolve blood viremia and persist for months in saliva, urine, semen, breast milk and the central nervous system and further developed Zika syndrome and Guillain-Barre syndrome(21,22). Therefore, outbreaks and epidemics of ZIKV are caused a huge threats to human health, especially pregnant women and newborns.…”

supporting

confidence: 48%

Utilizing network pharmacology to explore the underlying mechanism of Cinnamomi Ramulus in Zika Fever induced by Zika Virus

Zhang

Lin

Lai

et al. 2020

Preprint

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Background: Zika fever is a mosquito-borne infection induced by Zika virus (ZIKV),effective drug and vaccine against ZIKV infection is still unavailable. Cinnamomi ramulus (CR) is a traditional Chinese herb with a long history ,can stimulate secretion of sweat and relieve exterior syndrome. The aim of this research was to evaluate the effect of CR against ZIKV and uncover its mechanism of action by network pharmacology.Methods: Cell viability assay, qRT-PCR assay and western blot assays were perfermed to evaluate anti-ZIKV activity in vitro. Survival rate, body weight were observed and viremia was detected in AG6 mice. Drug- target-disease networks, GO enrichment, and KEGG pathway analysis was established to clarify the therapeutic mechanismsits. Results: In this study, we find that CR can alleviate CPE after Zika virus infection. In the premixed administration mode, CR showed superior activity to inhibit viral RNA replication and protein expression in cells. Orally administered CR effectively protected AG6 mice infected with lethal doses of ZIKV, conferring 50% or 20% survival rate at a dosage of 900mg/ml or 450mg/ml, reducing body weight loss, inhibiting viral RNA replication. Beta-sitosterol, DBP, α-Longipinen, (-)-alpha-cedrene, ()-alpha-Longipinene may the main active compound and PTGS2, GABRA1, PTGS1, PTGES and CCR5 may the main targets. 176 biological processes, 16 cell components, 27 molecular functions, and 37 pathways were significantly identified in GO enrichment and pathway analysis. Conclusions: These results reveal that CR treatment of Zika fever is mainly related to PTGS1 and PTGS2 regulated prostaglandin release, metabolism, and inflammation response.

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supporting

confidence: 48%

Utilizing network pharmacology to explore the underlying mechanism of Cinnamomi Ramulus in Zika Fever induced by Zika Virus

Zhang

Lin

Lai

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…The virus was cultured in Vero cells, inducing typical cytopathic effects and raising the possibility of transmission from an infected patient to a non-immune human host (44) . Interestingly, an earlier report exists of Zika virus transmission by a monkey bite in an Australian tourist returning from Indonesia, which was dismissed as improbable at the time (45) .…”

Section: Zika Virus: Old and Newly Identified Transmission Modesmentioning

confidence: 99%

Zika virus: what do we know about the viral structure, mechanisms of transmission, and neurological outcomes?

Silva

Souza

2016

Rev. Soc. Bras. Med. Trop.

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The Zika virus epidemic that started in Brazil in 2014 has spread to >30 countries and territories in Latin America, leading to a rapid rise in the incidence of microcephalic newborns and adults with neurological complications. At the beginning of the outbreak, little was known about Zika virus morphology, genome structure, modes of transmission, and its potential to cause neurological malformations and disorders. With the advancement of basic science, discoveries of the mechanisms of strain variability, viral transfer to the fetus, and neurovirulence were published. These will certainly lead to the development of strategies to block vertical viral transmission, neuronal invasion, and pathogenesis in the near future. This paper reviews the current literature on Zika virus infections, with the aim of gaining a holistic insight into their etiology and pathogenesis. We discuss Zika virus history and epidemiology in Brazil, viral structure and taxonomy, old and newly identified transmission modes, and neurological consequences of infection.

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“…Viral load was found to be higher in urine samples than saliva samples. The study also revealed that out of two isolated strains only one was isolated from urine suggesting other factors such as acidic nature of fluid also affects the infectivity of virion [45]. Detection of ZIKA virus in urine of patients more than 10 days after onset of disease have proved urine sample useful for diagnosis of ZIKV infections.…”

Section: Transmission Through Urinementioning

confidence: 97%

Advances in research on Zika virus

Ali

Wahid

Rafique

et al. 2017

Asian Pacific Journal of Tropical Medicine

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Zika virus (ZIKV) is rapidly spreading across the America and its devastating outcomes for pregnant women and infants have driven this previously ignored pathogen into the limelight. Clinical manifestations are fever, joint pain or rash and conjunctivitis. Emergence of ZIKV started with a first outbreak in the Pacific area in 2007, a second large outbreak occurred in the Pacific in 2013/2014 and subsequently the virus spread in other Pacific islands. Threat of explosive global pandemic and severe clinical complications linked with the more immediate and recurrent epidemics necessitate the development of an effective vaccine. Several vaccine platforms such as DNA vaccine, recombinant subunit vaccine, ZIKV purified inactivated vaccine, and chimeric vaccines have shown potent efficacy in vitro and in vivo trials. Moreover, number of drugs such as Sofosbuvir, BCX4450, NITD008 and 7-DMA are ready to enter phase I clinical trial because of proven anti-ZIKV activity. Monoclonal based antibodies offer promise as an intervention effective for use in pregnant women. In this review, we describe the advances in research on ZIKV such as research strategies for the development of antiviral drugs & vaccines, molecular evolution, epidemiology emergence, neurological complications and other teratogenic outcomes as well as pathogenesis.

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Isolation of infective Zika virus from urine and saliva of patients in Brazil

Abstract: Background

Cited by 36 publications

References 43 publications

Utilizing network pharmacology to explore the underlying mechanism of Cinnamomi Ramulus in Zika Fever induced by Zika Virus

Utilizing network pharmacology to explore the underlying mechanism of Cinnamomi Ramulus in Zika Fever induced by Zika Virus

Zika virus: what do we know about the viral structure, mechanisms of transmission, and neurological outcomes?

Advances in research on Zika virus

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