Isolation of
            <i>Serpulina pilosicoli</i>
            from Rectal Biopsy Specimens Showing Evidence of Intestinal Spirochetosis

Trivett-Moore, Nicolle L.; Gilbert, Gwendolyn L.; Law, Carmella; Trott, Darren J.; Hampson, D.J.

doi:10.1128/jcm.36.1.261-265.1998

Cited by 107 publications

(76 citation statements)

References 38 publications

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“…In contrast to previous studies, the routine use of PAS and Steiner silver stains did not increase the number of diagnosed cases in the present study. 17,33 In fact, the characteristic fuzzy blue 23,24 were amplified in biopsies of only four patients. In part, this low identification rate may have been due to the prolonged storage of the samples prior to PCR amplification in 2004, with resultant degradation of the DNA.…”

Section: Discussionmentioning

confidence: 91%

Intestinal spirochetosis and chronic watery diarrhea: Clinical and histological response to treatment and long‐term follow up

Esteve

Salas²,

Fernández‐Bañares

et al. 2006

J of Gastro and Hepatol

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Histological intestinal spirochetosis appears to be relatively uncommon in Catalonia (Spain) compared to previous reports from other countries, but was identified in patients (0.7%) with chronic watery diarrhea. Sustained clinical recovery after spontaneous or drug-induced spirochetal disappearance in these individuals suggests that intestinal spirochetosis may play a pathogenic role in chronic watery diarrhea. Treatment with metronidazole is advisable in patients with persistent symptoms.

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Section: Discussionmentioning

confidence: 91%

Intestinal spirochetosis and chronic watery diarrhea: Clinical and histological response to treatment and long‐term follow up

Esteve

Salas²,

Fernández‐Bañares

et al. 2006

J of Gastro and Hepatol

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“…No common organism has been cultured in a large number of cases of spirochetosis, but a Brachyspira aalborgi specific sequence was detected by PCR in 62.5% of patients with spirochetosis [6]. In another study Serpulina pilosicoli was recovered from rectal biopsies in 50% of symptomatic patients with a histological diagnosis of intestinal spirochetosis [7]. There is an increased incidence of spirochetosis in homosexual men affecting up to 30% without evidence of associated immunodeficiency [8].…”

Section: Introductionmentioning

confidence: 99%

Intestinal spirochetosis: clinicopathological features with review of the literature

Alsaigh

Fogt

2002

Colorectal Disease

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OBJECTIVE: The purpose of this study is to evaluate the clinical presentation of patients with intestinal spirochetosis, as it is has not been well described in the literature. METHODS: We studied 15 patients with colonic biopsies that showed spirochetosis. The study group consisted of 11 males and 4 females, 10 of the males were heterosexual and one was homosexual. None of these patients were clinically immunocompromized. A colonoscopy was performed for evaluation of different symptoms. The histological diagnosis of spirochetosis was made on H&E examination and confirmed by Giemsa and/or Steiner stain. There was no evidence of associated active or any specific colitis in any of the cases. The control group consisted of 30 patients, 16 males and 14 females that were matched for age and clinical indications for obtaining a colonic biopsy. RESULTS: Colonic mucosa with prominent brush border-like surface colonized with large amounts of spirochetes was noted in all 15 cases in the study group and in none of 30 cases in the control group. The clinical presentation in patients with spirochetosis was compared to the control group. Of the spirochetosis patients 86% presented with some form of clinical symptoms compared with 13% of controls. These symptoms included chronic watery diarrhoea in 40% spirochetosis patients vs 5% in controls; a change in bowel habit was present in 33% of spirochetosis patients vs 3.3% in controls. The endoscopic appearance in spirochetosis patients was reported as normal in six patients, 'polyoid' in seven patients, erythematous in one patient and 'lesion' in one patient. CONCLUSION: These findings suggest that colonic spirochetosis affects a heterogenous group of patients, the majority of whom presented with gastrointestinal symptoms. The variable clinical findings may be related to the infecting organism and the condition may not be harmless in all patients.

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“…Two species of anaerobic intestinal spirochaetes, Brachyspira (formerly Serpulina) pilosicoli [1] and Brachyspira aalborgi [2], colonise the human large intestine [3,4]. Both species are associated with a condition known as intestinal spirochaetosis (IS), in which a thin carpet-like layer of spirochaetes is found attached by one cell end to the colorectal surface epithelium [5].…”

Section: Introductionmentioning

confidence: 99%

“…B. pilosicoli has been isolated from human faeces or intestinal biopsies in a number of studies from the USA [7], Europe [8^10], Oman [11], Papua New Guinea [12], and Australia [3,9,13]. It also naturally infects pigs [1], dogs [12], and chickens [14].…”

Section: Introductionmentioning

confidence: 99%

PCR detection of Brachyspira aalborgi and Brachyspira pilosicoli in human faeces

Mikosza¹

2001

FEMS Microbiology Letters

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Previously-developed PCR protocols specific for the 16S rRNA gene of the intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli were adapted for the detection of these species in human faeces, following DNA extraction and purification using mini-prep columns. The limits of detection in seeded faeces for B. aalborgi and B. pilosicoli respectively were 2U10 2 and 7U10 3 cells per PCR reaction, equivalent to 5U10 4 and 1U10 5 cells per g of faeces. The PCR techniques were applied to faecal samples from two patients with histological evidence of intestinal spirochaetosis. In the first patient, in whom B. aalborgi had been identified by 16S rDNA PCR from colonic biopsies, a positive amplification for B. aalborgi only was obtained from the faeces. The organism could not be isolated from these faeces. In the second patient, both colonic biopsies and faeces were PCR positive for B. pilosicoli only, and B. pilosicoli was isolated from the faeces. These new faecal PCR protocols should be valuable for future studies on the epidemiology of intestinal spirochaete infections in human populations, particularly as it is not currently possible to isolate B. aalborgi from faeces. ß

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Isolation of Serpulina pilosicoli from Rectal Biopsy Specimens Showing Evidence of Intestinal Spirochetosis

Cited by 107 publications

References 38 publications

Intestinal spirochetosis and chronic watery diarrhea: Clinical and histological response to treatment and long‐term follow up

Intestinal spirochetosis and chronic watery diarrhea: Clinical and histological response to treatment and long‐term follow up

Intestinal spirochetosis: clinicopathological features with review of the literature

PCR detection of Brachyspira aalborgi and Brachyspira pilosicoli in human faeces

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