Isolation of<i>Pythium litorale</i>from irrigation ponds used for vegetable production and its pathogenicity on squash

Parkunan, Venkatesan; Ji, Pingsheng

doi:10.1080/07060661.2013.821423

Cited by 11 publications

(5 citation statements)

References 19 publications

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“…irrigata and Py. litorale have been show to produce disease on some vegetable crops in greenhouse studies, no disease in plants grown in the field has been attributed to these species (28,29). Other Phytophthora spp.…”

Section: Discussionmentioning

confidence: 99%

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Plant-Pathogenic Oomycetes, Escherichia coli Strains, and Salmonella spp. Frequently Found in Surface Water Used for Irrigation of Fruit and Vegetable Crops in New York State

Jones

Worobo

Smart

2014

Appl Environ Microbiol

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bIn the United States, surface water is commonly used to irrigate a variety of produce crops and can harbor pathogens responsible for food-borne illnesses and plant diseases. Understanding when pathogens infest water sources is valuable information for produce growers to improve the food safety and production of these crops. In this study, prevalence data along with regression tree analyses were used to correlate water quality parameters (pH, temperature, turbidity), irrigation site properties (source, the presence of livestock or fowl nearby), and precipitation data to the presence and concentrations of Escherichia coli, Salmonella spp., and hymexazol-insensitive (HIS) oomycetes (Phytophthora and Pythium spp.) in New York State surface waters. A total of 123 samples from 18 sites across New York State were tested for E. coli and Salmonella spp., of which 33% and 43% were positive, respectively. Additionally, 210 samples from 38 sites were tested for HIS oomycetes, and 88% were found to be positive, with 10 species of Phytophthora and 11 species of Pythium being identified from the samples. Regression analysis found no strong correlations between water quality parameters, site factors, or precipitation to the presence or concentration of E. coli in irrigation sources. For Salmonella, precipitation (<0.64 cm) 3 days before sampling was correlated to both presence and the highest counts. Analyses for oomycetes found creeks to have higher average counts than ponds, and higher turbidity levels were associated with higher oomycete counts. Overall, information gathered from this study can be used to better understand the food safety and plant pathogen risks of using surface water for irrigation.

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Section: Discussionmentioning

confidence: 99%

“…litorale. Each of these plant pathogens has previously been found in surface water sources and is not considered a major threat to fruit and vegetable growers (28)(29)(30). While Ph.…”

Section: Discussionmentioning

confidence: 99%

Plant-Pathogenic Oomycetes, Escherichia coli Strains, and Salmonella spp. Frequently Found in Surface Water Used for Irrigation of Fruit and Vegetable Crops in New York State

Jones

Worobo

Smart

2014

Appl Environ Microbiol

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“…Among the remaining oomycetes, Phytopythium litorale (Nechw.) Abad, de Cock, Bala, Robideau, Lodhi & Lévesque, Pythium mamillatum Meurs and Pythium dissotocum Drechsler have been reported as pathogens causing severe damping-off and root-rot diseases to different agricultural crops [54,79,80].…”

Section: Discussionmentioning

confidence: 99%

Fungi and Oomycetes in the Irrigation Water of Forest Nurseries

Marčiulynas

Marčiulynienė

Lynikienė

et al. 2020

Forests

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The aim of the present study was to assess fungal and oomycete communities in the irrigation water of forest nurseries, focusing on plant pathogens in the hope of getting a better understanding of potential pathogenic microorganisms and spreading routes in forest nurseries. The study sites were at Anykščiai, Dubrava, Kretinga and Trakai state forest nurseries in Lithuania. For the collection of microbial samples, at each nursery five 100-L water samples were collected from the irrigation ponds and filtered. Following DNA isolation from the irrigation water filtrate samples, these were individually amplified using ITS rDNA as a marker and subjected to PacBio high-throughput sequencing. Clustering in the SCATA pipeline and the taxonomic classification of 24,006 high-quality reads showed the presence of 1286 non-singleton taxa. Among those, 895 were representing fungi and oomycetes. The detected fungi were 57.3% Ascomycota, 38.1% Basidiomycota, 3.1% Chytridiomycota, 0.8% Mucoromycota and 0.7% Oomycota. The most common fungi were Malassezia restricta E. Guého, J. Guillot & Midgley (20.1% of all high-quality fungal sequences), Pezizella discreta (P. Karst.) Dennis (10.8%) and Epicoccum nigrum Link (4.9%). The most common oomycetes were Phytopythium cf. citrinum (B. Paul) Abad, de Cock, Bala, Robideau, Lodhi & Lévesque (0.4%), Phytophthora gallica T. Jung & J. Nechwatal (0.05%) and Peronospora sp. 4248_322 (0.05%). The results demonstrated that the irrigation water used by forest nurseries was inhabited by a species-rich but largely site-specific communities of fungi. Plant pathogens were relatively rare, but, under suitable conditions, these can develop rapidly, spread efficiently through the irrigation system and be a threat to the production of high-quality tree seedlings.

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“…Evidence for plant pathogenicity of P. litorale is mixed. Phytopythium litorale is commonly isolated from irrigation ponds in Georgia where it was shown to cause seedling damping-off and fruit rot of squash (Cucurbita pepo) (Parkunan and Ji, 2013), but isolates from greenhouse water tanks in Pennsylvania were not pathogenic in assays with geranium (Pelargonium ·hortorum) seedlings (Choudhary et al, 2016). It is not known if P. litorale is causing disease in Nursery B.…”

Section: Case Studies Nursery a (Oregon) Nurserymentioning

confidence: 99%

Hazard Analysis for Phytophthora Species in Container Nurseries: Three Case Studies

Parke¹,

Redekar²,

Eberhart³

et al. 2019

hortte

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Phytophthora species cause crop losses and reduce the quality of greenhouse and nursery plants. Phytophthora species can also be moved long distances by the plant trade, potentially spreading diseases to new hosts and habitats. Phytosanitary approaches based on quarantines and endpoint inspections have reduced, but not eliminated, the spread of Phytophthora species from nurseries. It is therefore important for plant production facilities to identify potential sources of contamination and to take corrective measures to prevent disease. We applied a systems approach to identify sources of contamination in three container nurseries in Oregon, California, and South Carolina. Surface water sources and recaptured runoff water were contaminated with plant pathogenic species at all three nurseries, but one nursery implemented an effective disinfestation treatment for recycled irrigation water. Other sources of contamination included cull piles and compost that were incorporated into potting media, infested soil and gravel beds, used containers, and plant returns. Management recommendations include preventing contact between containers and contaminated ground, improving drainage, pasteurizing potting media ingredients, steaming used containers, and quarantine and testing of incoming plants for Phytophthora species. These case studies illustrate how recycled irrigation water can contribute to the spread of waterborne pathogens and highlight the need to implement nursery management practices to reduce disease risk.

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Isolation ofPythium litoralefrom irrigation ponds used for vegetable production and its pathogenicity on squash

Cited by 11 publications

References 19 publications

Plant-Pathogenic Oomycetes, Escherichia coli Strains, and Salmonella spp. Frequently Found in Surface Water Used for Irrigation of Fruit and Vegetable Crops in New York State

Plant-Pathogenic Oomycetes, Escherichia coli Strains, and Salmonella spp. Frequently Found in Surface Water Used for Irrigation of Fruit and Vegetable Crops in New York State

Fungi and Oomycetes in the Irrigation Water of Forest Nurseries

Hazard Analysis for Phytophthora Species in Container Nurseries: Three Case Studies

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