Isolation of<i>Neisseria meningitides</i>from the Vagina and Cervix

Lewis, Jay F.; Alexander, Jane

doi:10.1093/ajcp/61.2.216

Cited by 18 publications

(12 citation statements)

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“…However, when we inoculated a strain of Pseudomonas aeruginosa on to the pads it grew well; the same applied to a strain of N. meningitidis and to commensal neisseriae inoculated from broth cultures. Microcult is thus clearly totally unsuitable for examining throat swabs for gonococci, and N. meningitidis has occasionally been isolated from the genital area (Lewis and Alexander, 1974). Furthermore, since it is becoming apparent that oxidasepositive Gram-negative bacteria of genera other than Neisseria spp may inhabit the genital tract (Platt and Snell, 1976) it would seem mandatory to isolate and identify as N. gonorrhoeae all oxidase-positive organisms growing in the Microcult system before reporting to the venereologist.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Microcult system for isolating and identifying Neisseria gonorrhoeae.

Williams¹,

Ratnatunga²,

Hamilton-Miller³

et al. 1978

J Clin Pathol

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SUMMARY Specimens from 95 patients attending a venereal diseases clinic were examined for gonococci by three methods-a conventional culture technique using modified Thayer-Martin medium, microscopy of a Gram-stained direct smear, and the Microcult system. For 56?' of the specimens the results by all three methods agreed. Assuming the results obtained by culture on Thayer-Martin medium to be correct, the largest source of error was due to false-positive results: microscopy gave 26 and Microcult gave 15 such results. False-negative results were less common: Microcult gave 14, microscopy six. Microcult gave positive results more quickly than the conventional Thayer-Martin cultural method, but the gonococci were difficult to isolate by subculture from the Microcult culture pads. The Microcult medium was not absolutely specific for Neisseria gonorrhoeae. Nevertheless, the Microcult test may well prove to be a useful adjunct to the diagnosis of gonorrhoea, especially when laboratory facilities are not readily available.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Microcult system for isolating and identifying Neisseria gonorrhoeae.

Williams¹,

Ratnatunga²,

Hamilton-Miller³

et al. 1978

J Clin Pathol

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“…However, N. meningitidis can be sporadically pathogenic in the genitourinary tract, as first reported by Murray in 1939 [82]. In several subsequent reports, the presence of N. meningitidis in the urethra was not associated with genital symptoms [78,83,84,95]. However, genital infections caused by meningococci may sometimes present similar clinical symptoms to classical gonorrhea, e.g., purulent penile discharge and urethritis, and cervicitis/vaginitis [29,30,[85][86][87]93,94] (Table 1).…”

Section: Meningococcal Genitourinary Tract Infectionsmentioning

confidence: 90%

Atypical, Yet Not Infrequent, Infections with Neisseria Species

Humbert

Christodoulides

2019

Pathogens

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Neisseria species are extremely well-adapted to their mammalian hosts and they display unique phenotypes that account for their ability to thrive within niche-specific conditions. The closely related species N. gonorrhoeae and N. meningitidis are the only two species of the genus recognized as strict human pathogens, causing the sexually transmitted disease gonorrhea and meningitis and sepsis, respectively. Gonococci colonize the mucosal epithelium of the male urethra and female endo/ectocervix, whereas meningococci colonize the mucosal epithelium of the human nasopharynx. The pathophysiological host responses to gonococcal and meningococcal infection are distinct. However, medical evidence dating back to the early 1900s demonstrates that these two species can cross-colonize anatomical niches, with patients often presenting with clinically-indistinguishable infections. The remaining Neisseria species are not commonly associated with disease and are considered as commensals within the normal microbiota of the human and animal nasopharynx. Nonetheless, clinical case reports suggest that they can behave as opportunistic pathogens. In this review, we describe the diversity of the genus Neisseria in the clinical context and raise the attention of microbiologists and clinicians for more cautious approaches in the diagnosis and treatment of the many pathologies these species may cause.

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“…This observation may be sufficient to support the use of 1,2-propanediol oxidoreductase as a presumptive diagnostic marker for N. gonorrhoeae. Recent papers (Blackwell et al, 1977 ;Faur et al, 1975 ;Lewis & Alexander, 1974;Givan et al, 1977), however, have reported increased isolations of non-gonococcal Neisseria species from the genital tract. This may lead to increased cross-reactivity.…”

Section: Discussionmentioning

confidence: 99%