The Occurrence of 1,2-Propanediol Oxidoreductase in Micro-organisms and its Use as a Possible Diagnostic Marker for Neisseria gonorrhoeae

Takeguchi, Milton M.; Livsey, Kim A.; Detar, Clarence C.; McDonald, Hugh C.; Smith, David K.; Simon, Paula A.; Weetall, Howard H.

doi:10.1099/00221287-119-2-459

Cited by 4 publications

(2 citation statements)

References 10 publications

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“…Recently, alternative approaches to the diagnosis of gonorrhoea, based upon the detection of gonococcal components in clinical specimens have been developed. These include detection of antigen in urine by solid-phase radioimmunoassay (Thornley et al, 1979), detection of gonococcal DNA by genetic transformation (Bawdon, Juni and Britt, 1977), the Limulus amoebocyte lysate assay for endotoxin Perkins, 1979 and, gas liquid chromatography (Sud and Feingold, 1979), and the detection of the enzyme 1,2-propanediol oxidoreductase (Takeguchi et al, 1980).…”

Section: Introductionmentioning

confidence: 99%

Non-cultural detection of Neisseria gonorrhoeae in cervical and vaginal washings

Young

Sarafian

Harris

et al. 1983

Journal of Medical Microbiology

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SUMMARY. Genetic transformation, an indirect sandwich enzymelinked immunosorbent assay (ELISA) and the Limulus amoebocyte assay were used to indicate the presence of products of Neisseria gonorrhoeae in vaginal and uterine cervical aspirates from 37 women attending a Department of Genito-Urinary Medicine. In parallel with these tests, qualitative and quantitative assessments of the microbial content of aspirates were made. There was wide variation in the numbers of gonococci cultured. The mean viable count for cervical aspirates was 1 x 1 O6 cfu/ml and the range was (5 x 103)--(8 x lo6) cfu/ml; the mean count for vaginal aspirates was 8.4 x lo4 cfu/ml and the range (1 x 102)-(1 x lo6) cfu/ml. Viable counts of organisms other than gonococci in vaginal aspirates were two to tenfold greater than the corresponding counts for cervical aspirates. Of 20 patients with gonorrhoea confirmed by conventional diagnostic cultures, aspirates from 15 (75%) gave a positive transformation result, and 12 (60%) a positive ELISA result; 16 (84.2%) out of 19 of these aspirates tested by the Limulus lysate assay were positive at a dilution of 1 in 100.

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Section: Introductionmentioning

confidence: 99%

Non-cultural detection of Neisseria gonorrhoeae in cervical and vaginal washings

Young

Sarafian

Harris

et al. 1983

Journal of Medical Microbiology

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“…2 Examination of various microorganisms in the female genital tract showed that appreciable concentrations of this particular enzyme were present in only Neisseria and Acinetobacter species.2 Of these organisms, N gonorrhoeae showed higher levels of activity than most of the other organisms. We decided therefore to use the enzymatic approach for the presumptive diagnosis of gonorrhoea in a clinical context.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic detection of Neisseria gonorrhoeae.

Takeguchi¹,

Weetall²,

Smith³

et al. 1980

Sexually Transmitted Infections

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SUMMARY In a study using a non-serological enzymatic approach for the detection of Neisseria gonorrhoeae in cervical and urethral swabs, the technique was shown to be technically feasible. The enzyme, 1, 2-propanediol oxidoreductase, was used as a presumptive diagnostic marker for N gonorrhoeae. Enzymatic activity was measured with a fluorometer. Two assay procedures were performed: (a) enzyme detection (two-tube and three-tube assays) requiring 60 minutes; and (b) enzyme inhibition (EI) (90-minute and modified 20-minute assays). Sensitivities of the two-tube, three-tube, and the 90-minute EI assays with male urethral specimens from a high-prevalence population were 80'7o, 84%, and 81 % respectively. The specificities of these assays in a lowprevalence male population were not determined. Sensitivity of the 90-minute EI assay in a highprevalence female group was 77% and specificity in a low-prevalence female group was 75%. The modified EI assay was tested only in a low-prevalence female group and had 87%o specificity. Although the specificity of the assays needs improvement, several advantages-including early case detection, rapid availability of results, detection of current active infections, and the possibility of automation-are intrinsic in this enzymatic approach.

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Detection of Neisseria gonorrhoeae by ultraviolet illumination of NADH generated by an immobilized antibody-gonococcal enzyme complex

McDonald¹,

Simon²,

Takeguchi³

et al. 1981

Immunology Letters

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The Occurrence of 1,2-Propanediol Oxidoreductase in Micro-organisms and its Use as a Possible Diagnostic Marker for Neisseria gonorrhoeae

Cited by 4 publications

References 10 publications

Non-cultural detection of Neisseria gonorrhoeae in cervical and vaginal washings

Non-cultural detection of Neisseria gonorrhoeae in cervical and vaginal washings

Enzymatic detection of Neisseria gonorrhoeae.

Detection of Neisseria gonorrhoeae by ultraviolet illumination of NADH generated by an immobilized antibody-gonococcal enzyme complex

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