Isolation of Î‘-Glucosidase Inhibitory Active Compounds From Ethanol Extract of Kayu Tuah (Antidesma Celebicum Miq.) Leaves

Novi, Fajar Utami; Elya, Berna; Katrin,

doi:10.7897/2230-8407.0615

Cited by 2 publications

(3 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It can be concluded that the total phenol content in EEAC was 18.712 ppm or 187.12 g GAE [ 27 ]. EEAC is known to have an antidiabetic effect by inhibiting α -glucosidase activity mainly due to its phenolic content (i.e., gallic acid) [ 10 ]. Phenols can control starch digestion by forming hydrogen bonds with polar digestive enzymes such as α -amylase and α -glucosidase.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, EEAC is known to have α -glucosidase inhibitory activity whose mechanism of action is similar to that of acarbose [ 10 ]. Oral administration of EEAC for 28 days drastically reduced blood glucose level compared to the diabetic control group, and this reduction in FBG level was similar to the acarbose-treated group.…”

Section: Resultsmentioning

confidence: 99%

“…Ethanolic extract of A. celebicum leaves (EEAC) is known to contain flavonoids, tannins, saponins, and glycosides [ 9 ]. Novi et al [ 10 ] succeeded in isolating several active compounds from AC leaves that had α -glucosidase inhibitory activity, namely gallic acid; 3, 4, 5-trihydroxy-1-methoxybenzene; dioctyl phthalates; and dienoic acid. In vitro testing of the four compounds' activity against α -glucosidase inhibition showed an IC value of 0.057, 0.077, 0.206, and 0.182 mM, respectively, while the comparison control, acarbose, showed an IC50 value of 0.005 mM.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antidiabetic Properties and Toxicological Assessment of Antidesma celebicum Miq: Ethanolic Leaves Extract in Sprague–Dawley Rats

Pratiwi

Elya

Setiawan

et al. 2022

Advances in Pharmacological and Pharmaceutical Sciences

View full text Add to dashboard Cite

Antidesma is a genus of plants, and its several species are known to have antidiabetic properties. Leaves of Kayu Tuah (Antidesma celebicum Miq) have been proven to have the best α-glucosidase inhibition ability compared to other species in the Antidesma genus, as evidenced by the in vitro α-glucosidase inhibition test. However, no scientific studies have reported its antidiabetic properties and toxicity in vivo. Therefore, this research managed to verify the antidiabetic features and safety of ethanolic extract of A. celebicum leaves (EEAC) in Sprague–Dawley rats. Male rats (170–280 g) were induced diabetic with streptozotocin (35 mg/kg BW) and fed a high-fat diet comprising 24% fat, whereas control group rats were given a standard diet. Rats were treated with EEAC at 200 and 400 mg/kg BW doses for 28-days and 60 mg/kg BW acarbose for the control group. Determination of antidiabetic properties was done by analyzing lipid profiles as well as fasting blood glucose. After confirming the antidiabetic properties of EEAC, the toxicological assessment was determined using the fixed-dose method. General behavior changes, appearance, signs of toxicity, mortality, and body weight of animals were marked down during the observation period. When the treatment period ended, hematological, biochemical, and histological examinations of liver, kidneys, and heart sections were performed. The results confirmed that EEAC reduced fasting blood glucose levels and stepped forward lipid profiles of rats. Also, all animals survived, and no obvious destructive outcomes were noticeable during the study. As EEAC has promising results toward hyperglycemia and hyperlipidemia and has been proven safe through toxicity tests, it can be concluded that EEAC has good potential to be further developed into antidiabetic drugs.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antidiabetic Properties and Toxicological Assessment of Antidesma celebicum Miq: Ethanolic Leaves Extract in Sprague–Dawley Rats

Pratiwi

Elya

Setiawan

et al. 2022

Advances in Pharmacological and Pharmaceutical Sciences

View full text Add to dashboard Cite

show abstract

α-Glucosidase inhibitors and phytotoxins from Streptomyces xanthophaeus

Wei

Zhang

Deng

et al. 2016

Natural Product Research

View full text Add to dashboard Cite

Twenty-four metabolites 1-24 were isolated from the fermentation broth of Streptomyces xanthophaeus. Their structures were elucidated on the basis of spectroscopic analysis and by comparison of their NMR data with literature data reported. Daidzein (1), genistein (2) and gliricidin (3) inhibited α-glucosidase in vitro with IC values of 174.2, 36.1 and 47.4 μM, respectively, more potent than the positive control, acarbose. Docking study revealed that the amino acid residue Thr 215 is the essential binding site for active ligands 2. In addition, the phytotoxic effects of all compounds were assayed on radish seedlings, five of which, 3, 8, 13, 15 and 18, inhibited the growth of radish (Raphanus sativus) seedlings with inhibitory rates of >60% at a concentration of 100 ppm, which was comparable or superior to the positive control glyphosate. This is the first report of the phytotoxicity of the compounds.

show abstract

Isolation of Î‘-Glucosidase Inhibitory Active Compounds From Ethanol Extract of Kayu Tuah (Antidesma Celebicum Miq.) Leaves

Cited by 2 publications

References 11 publications

Antidiabetic Properties and Toxicological Assessment of Antidesma celebicum Miq: Ethanolic Leaves Extract in Sprague–Dawley Rats

Antidiabetic Properties and Toxicological Assessment of Antidesma celebicum Miq: Ethanolic Leaves Extract in Sprague–Dawley Rats

α-Glucosidase inhibitors and phytotoxins from Streptomyces xanthophaeus

Contact Info

Product

Resources

About