Isolation of human pathogenic viruses from clinical material on CaCo2 cells

Identification of enteroviral infection among infants and children admitted to hospital with acute gastroentritis in Ho Chi Minh City, Vietnam

“…These findings are different from other studies where enterovirus have been isolated during the rainy season in tropical countries such as Vietnam [Melnick, 1996;Mark and Raymond, 2001]. There are several different cell lines used for the cultivation of enteroviruses [Reigel, 1985;Chezzi, 1996;AbuBakar et al, 1999;Munemura et al, 2003;MoceLlivina et al, 2004]. The use of the epidermal-like cell line CaCo2 and the efficient propagation of viruses from clinical material for diagnostic purposes have been reported [Reigel, 1985].…”

“…There are several different cell lines used for the cultivation of enteroviruses [Reigel, 1985;Chezzi, 1996;AbuBakar et al, 1999;Munemura et al, 2003;MoceLlivina et al, 2004]. The use of the epidermal-like cell line CaCo2 and the efficient propagation of viruses from clinical material for diagnostic purposes have been reported [Reigel, 1985]. Fecal specimens were used for virus isolation using two cell lines CaCo2 and Vero.…”

Engineered BGMK Cells for Sensitive and Rapid Detection of Enteroviruses

“…CaCo-2, a human colon adenocarcinoma cell line, has been demonstrated to support the replication of, and show visible CPE for, most enteroviruses (12). On the basis of this report and the evidence that only 13 types of echoviruses use hDAF as a receptor, a mixed monolayer of BGMK-hDAF and CaCo-2 cells might provide a broader range of enterovirus detection in a shorter time than other single-or multi-cell-line monolayers.…”

“…The echoviruses that hemagglutinate human erythrocytes, including serotypes 3,6,7,11,12,13,19,21,24,25,29,30, and 33, bind to DAF receptor in human cells, and the binding can be blocked by an anti-DAF monoclonal antibody (MAb) (1,11). In addition, coxsackievirus A21 also binds to DAF of HEp-2 and HeLa cells, and the binding of coxsackieviruses B1, B3, and B5 to HeLa cells can be blocked by an anti-DAF MAb (2,14,15).…”

Enterovirus Infections in Germany: Comparative Evaluation of Different Laboratory Diagnostic Methods

“…Virus isolation was attempted from stool and CSF samples as described [4,5], using the human colonic cancer cell line CaCo-2 (European Collection of Cell Cultures, Salisbury, United Kingdom). Isolated viruses were typed by immunofluorescence using monoclonal antibodies (Virion, Planegg, Germany).…”