Isolation of Helicobacter pylori from feces of patients with dyspepsia in the United Kingdom

Kelly, Sean M.; Pitcher, Maxton; Farmery, Susan M.; Gibson, Glenn R.

doi:10.1016/0016-5085(94)90806-0

Cited by 224 publications

(133 citation statements)

References 11 publications

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“…The fact that good personal hygiene and a low rate of geophagia scored high in Component 2 is explained, most likely, by the fact that older persons are less likely to practice unhygienic or geophagic behaviours than children. Infection with H. pylori appears to be acquired early in childhood (Mitchell et al 1992;Webb et al 1994), and it has been postulated that this occurs via a faeco-oral pathway (Mapstone et al 1993;Kelly et al 1994;Sathar et al 1997). On the other hand, some workers have presented evidence against spread of Helicobacter spp.…”

Section: Discussionmentioning

confidence: 99%

“…Thus far, it is generally agreed that the bacterium is transmitted from person to person (Goodman & Correa 1995): identification of H. pylori in dental plaques and saliva (Banatvala et al 1993;Ferguson et al 1993;Mapstone et al 1993) suggests an oral-oral pathway. The presence of H. pylori in faeces (Mapstone et al 1993;Kelly et al 1994) and in institutionalized children (Blazer 1990) and the close epidemiological association between H. pylori and hepatitis A infections (Sathar et al 1997) are indicative of faeco-oral transmission (Hazel et al 1994). Familial clustering of infections (Mitchell et al 1987;Drumm et al 1990) and the occurrence of genetically similar isolates of H. pylori amongst family members (Bamford et al 1993) point to commonsource infection.…”

Section: Introductionmentioning

confidence: 99%

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Seroepidemiology of Helicobacter pylori infection in a Jamaican community

Lindo

Lyn-Sue

Palmer

et al. 1999

Tropical Med Int Health

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SummaryWe researched epidemiologic associations between environmental and demographic factors and prevalence of Helicobacter pylori infection in a suburban Jamaican community. Using a clustered sampling technique, 22 domestic yards enclosing 60 separate households were randomly selected from a local community. All household members (n ϭ 346) were invited to participate following informed consent; the overall compliance rate was 58.9%. A commercial enzyme immunoassay (HM·CAP) was used to detect IgG antibodies raised against H. pylori. Environmental and demographic information was obtained by questionnaire. The seroprevalence of H. pylori was 69.9% (n ϭ 202). Analysis of the independent variables revealed three major components: Component 1 described, collectively, good personal hygiene and sanitation, indoor water supply and absence of straying animals in the peridomestic area; Component 2 included older age, good personal hygiene and large yard size; Component 3 the presence of domestic animals (cats and dogs) and, again, large yard size. These three complexes explained 42.2% of the variability in the data set. Logistic regression showed that Components 2 and 3 were independently associated with H. pylori seropositivity, indicating that a combination of demographic, environmental and zoonotic factors is involved in the spread of H. pylori infections at the tropical community level.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Seroepidemiology of Helicobacter pylori infection in a Jamaican community

Lindo

Lyn-Sue

Palmer

et al. 1999

Tropical Med Int Health

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“…Tissue and bacterial DNA was extracted with the QIAamp DNA minikit (Qiagen, Hilden, Germany) according to the manufacturer's recommendations with minor modifications. 25 Genomic DNA was PCR-amplified for cagA by using 2 sets of synthetic oligonucleotides primers as described by Kelly et al 26 and Peek et al 27 The amplified PCR products were resolved in 1% agarose gels containing tris/borate/EDTA by using 100 bp (Gibco-BRL, Gaithersburg, MD) as a molecular weight marker. The agarose gels were stained with ethidium bromide and viewed under short-wavelength UV light.…”

Section: Pcr Analysismentioning

confidence: 99%

IL1RN polymorphic gene and cagA‐positive status independently increase the risk of noncardia gastric carcinoma

Rocha

Guerra

Rocha

et al. 2005

Intl Journal of Cancer

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Helicobacter pylori cagA-positive strains and host cytokine proinflammatory polymorphisms have been associated with gastric carcinoma. However, the individual role of each factor has not been evaluated yet. Our aim was to evaluate whether IL-1 gene cluster and tumor necrosis factor-a (TNFA)-307 polymorphisms, as well as cagA-positive status, are associated with gastric carcinoma in a non-Caucasian population by analyzing the data in logistic regression models. We evaluated 166 patients with noncardia gastric carcinoma and 541 blood donors. Among them, 702 were successfully genotyped for all cytokine studied: 166 with gastric carcinoma and 536 controls. The carcinoma patients were considered to be H. pylori-positive if culture alone or 2 among preformed urease test, stained smear or histologic section, serology, polymerase chain reaction (PCR) for ureA and urea breath test were positive. In blood donors, H. pylori status was based on enzyme-linked immunosorbent assay. The cagA status was determined by PCR or serology. IL1B-511/-31, IL1RN (interleukin-1 receptor antagonist) and TNFA-307 polymorphisms were genotyped by PCR, PCR with restriction fragment length polymorphism, or PCR with confronting 2-pair primers. We found that the IL1RN2 polymorphic allele (OR 5 1.93) was associated with noncardia gastric carcinoma, even after inclusion of age, gender and cagA status in the logistic models. However, the cagA-positive status was the strongest independent factor associated with gastric carcinoma (OR 5 11.89). The other polymorphisms were not significantly associated with the disease when they were evaluated in logistic models. This study provides evidence supporting the independent associations of cagA-positive H. pylori status and IL1RN polymorphisms with noncardia gastric carcinoma. ' 2005 Wiley-Liss, Inc.

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“…pylori HP1 was recovered in modified CBA, and H. pylori ATCC 43629 in CBA and in HPSPA (Helicobacter pylori Special Peptone Agar) supplemented with vancomycin (10 mg/l), amphotericin (5 mg/l), cefsulodin (10 mg/l), polymyxin B sulfate (62,000 IU/l), trimethoprim (40 mg/l) and sulfamethoxazole (20 mg/l) (14,18). HPSPA and Columbia agar without supplements were also tested for the recovery of H. pylori ATCC.…”

Section: Culture Mediamentioning

confidence: 99%

“…Difficulties in elucidating the transmission routes of H. pylori are partially due to unsuitable detection methods available for the recovery of this pathogen from samples with a dense competing microflora (14,17,18). In this work we evaluated selective and non-selective media for the recovery of H. pylori from vegetable foods.…”

Section: Introductionmentioning

confidence: 99%

Fate of Helicobacter pylori artificially inoculated in lettuce and carrot samples

Gomes¹,

Martinis²

2004

Braz. J. Microbiol.

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Helicobacter pylori is known, worldwide, as the causative agent of gastric diseases. However, its transmission route has not been completely understood. To evaluate the survival of H. pylori (a clinical and a reference strain) artificially inoculated on lettuce and carrot samples, portions of 25 g were inoculated with approximately 10 6 CFU/g of H. pylori and packed under normal and/or modified atmosphere (3% oxygen, 10% carbon dioxide, and 87% nitrogen). The inoculated food samples were stored at 8ºC, with daily enumeration of H. pylori populations on Columbia blood agar (CBA) and/ or Helicobacter pylori Special Peptone Agar (HPSPA). When CBA with antibiotics was used, the clinical isolate H. pylori HP1 was detected for up to 72 h in sanitized lettuce and carrot. In sterilized carrot samples, H. pylori HP1 remained viable for up to 96 h. The CBA without antibiotics allowed the recovery of H. pylori ATCC 43629, from carrot samples stored in both atmospheres tested, for up to 120 hours. Our results reinforce that foodborne transmission of H. pylori cannot be disregarded yet.

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Isolation of Helicobacter pylori from feces of patients with dyspepsia in the United Kingdom

Cited by 224 publications

References 11 publications

Seroepidemiology of Helicobacter pylori infection in a Jamaican community

Seroepidemiology of Helicobacter pylori infection in a Jamaican community

IL1RN polymorphic gene and cagA‐positive status independently increase the risk of noncardia gastric carcinoma

Fate of Helicobacter pylori artificially inoculated in lettuce and carrot samples

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