Isolation of Harveyi clade Vibrio spp. collected in aquaculture farms: How can the identification issue be addressed?

Culot, Antoine; Grosset, Noël; Bruey, Quentin; Auzou, Michel; Giard, Jean‐Christophe; Favard, Baptiste; Wakatsuki, Alexandre; Baron, Sandrine; Frouel, Stephane; Techer, Clarisse; Gautier, Michel

doi:10.1016/j.mimet.2020.106106

Cited by 16 publications

(7 citation statements)

References 60 publications

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“…Thus, the results reinforce the need to use additional phenotypical or molecular techniques in order to confirm the identification of isolates in this culture medium (Culot et al . 2020; Canellas et al . 2021).…”

Section: Resultsmentioning

confidence: 99%

Diving into the unknown: identification of antimicrobial resistance hotspots in a tropical urban estuary

Canellas

Costa

Paranhos

et al. 2021

Lett Appl Microbiol

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Antimicrobial resistance is widely studied and well‐characterized from a clinical perspective. However, considerably less information is available regarding resistance in environmental settings, especially in aquatic habitats. This study presents data regarding the occurrence, distribution and the antimicrobial susceptibility profile of bacteria isolated from Guanabara Bay (GB), a heavily polluted tropical urban estuary and an important tourist attraction in Rio de Janeiro, Brazil. Water samples from sites characterized by growing degrees of pollution were analysed by culture‐dependent methods, revealing the presence of multidrug‐resistant bacteria and clinically relevant indicators of antimicrobial resistance, such as extended‐spectrum beta‐lactamases. Isolates were identified by mass spectrometry, which indicated the presence of potential human pathogens such as Aeromonas spp. and Vibrio spp. Bacteria harbouring beta‐lactam resistance genes were also detected. Although GB is widely used as a recreational and fishing area, there is a substantial knowledge gap regarding the monitoring of antimicrobial resistance and the risk that exposure to these waters poses to public health. Thus, this study reveals new information that calls for better comprehension of antimicrobial resistance in aquatic environments, especially those used for recreational purposes.

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Section: Resultsmentioning

confidence: 99%

Diving into the unknown: identification of antimicrobial resistance hotspots in a tropical urban estuary

Canellas

Costa

Paranhos

et al. 2021

Lett Appl Microbiol

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“…Bakteri Vibrio sp. diidentifikasi adalah jenis strain bakteri yang dapat memicu timbulnya penyakit early mortality syndrome (EMS) dan acute hepatopancreatic necrotic disease (AHPND) pada udang vaname (Culot et al, 2021). Bakteri Vibrio sp.…”

Section: Hasil Dan Bahasan Kelimpahan Bakteri Vibrio Sp Dan Total Bak...unclassified

ANALISIS PERMODELAN DINAMIS KELIMPAHAN BAKTERI Vibrio sp. PADA BUDIDAYA UDANG VANAME, Litopenaeus vannamei

Ariadi

Mujtahidah

2022

Jurnal Riset Akuakultur

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Vibrio sp. adalah jenis bakteri patogen yang banyak ditemui pada tambak budidaya udang. Spesies bakteri ini banyak menyerang udang vaname hingga menimbulkan mortalitas. Tujuan dari penelitian ini adalah untuk mengestimasi dinamika kelimpahan bakteri Vibrio sp. pada tambak budidaya udang vaname, Litopenaeus vannamei berdasarkan analisis permodelan dinamis. Penelitian ini dilakukan pada lima petakan tambak budidaya udang vaname berukuran 2.000 m2 selama 30 hari pada masa awal pemeliharaan, dengan indikator kelimpahan bakteri Vibrio sp., total bakteri, serta parameter kualitas air sebagai data variabel pengamatan. Data dianalisis dengan menggunakan software Stella ver.12. Berdasarkan hasil pengamatan, menunjukkan bahwa tingkat kelimpahan Vibrio sp. dipetakan tambak sebanyak 6,1 x 101 CFU/mL; dan total bakteri 1,421 x 103 CFU/mL. Kemudian untuk parameter kualitas air budidaya masih cukup baik dengan rata-rata nilai pH 8,0 ± 0,12; salinitas 27 ± 0,15 ppt; oksigen terlarut 5,85 ± 1,17 mg/L; suhu 28,79 ± 0,25°C; TAN 1 ± 3,35 mg/L; bahan organik 104,28 ± 5,43 mg/L. Selain itu, fluktuasi Vibrio sp. memiliki pola yang mirip dengan total kelimpahan bakteri, tetapi dengan nilai fluktuasi yang berlawanan. Selanjutnya, dari estimasi permodelan dinamis kelimpahan bakteri Vibrio sp. berlangsung secara dinamis dengan titik kelimpahan tertinggi sebanyak 4 x 103 CFU/mL mengikuti pengaruh dari aktivitas metabolisme dan tingkat adaptasi terhadap kondisi lingkungan. Berdasarkan causal loop model digambarkan faktor limbah dan nutrien adalah faktor yang memengaruhi tingkat metabolisme, serta kelimpahan bakteri di perairan. Nampaknya, tingkat kelimpahan bakteri Vibrio sp. pada tambak budidaya udang vaname, L. vannamei yang diestimasi memiliki fluktuasi yang dinamis mengikuti pola tingkat metabolisme biologis bakteri itu sendiri, serta kondisi lingkungan sekitar, yang memungkinkan tingkat infeksi bakteri ini akan menjadi semakin oportunis seiring dengan lamanya siklus budidaya udang.Vibrio sp. is a group of pathogenic bacteria commonly found in shrimp culture ponds. These bacteria could infect and cause mass mortality in cultured vaname shrimp. The purpose of this study was to estimate the abundance dynamics of Vibrio sp. in culture ponds of vaname shrimp (L. vannamei) based on dynamic modeling analysis. The measurement was conducted on five vaname shrimp ponds, each sized 2,000 m2 starting from the beginning until 30 days of rearing. The measured parameters were the abundance of Vibrio sp., total bacterial and water quality parameters. The data were analyzed using Stella ver.12 software. The observation revealed that the abundance of Vibrio sp. and total bacterial count in the ponds were 6.1 x 101 CFU/mL and 1.421 x 103 CFU/mL, respectively. The variations of water quality parameters were considered good with the average pH value of 8.0 ± 0.12, salinity of 27 ± 0.15 ppt, dissolved oxygen of 5.85 ± 1.17 mg/L, temperature of 28.79 ± 0.25°C, TAN of 1 ± 3.35mg/L, and organic matter of 104.28 ± 5.43 mg/L. As expected, Vibrio sp. fluctuations were diametrically opposed to the total bacterial abundance. Based on the performed dynamic modeling, the Vibrio sp. abundance occurs dynamically with the highest abundance of 4 x 103 CFU/mL following the influence of metabolic activity and the level of adaptation to environmental conditions. Based on the causal loop model, waste, and nutrient factors are considered factors that affect the metabolic rate and abundance of bacteria in the waters. Therefore, this study reconfirms that the abundance of Vibrio sp. in vaname shrimp (L. vannamei) culture ponds is strongly influenced by the dynamic fluctuation patterns of the biological metabolic rate of bacteria itself and environmental conditions, which allows the infection level of these bacteria to become more opportunistic as the length of shrimp culture period increases.

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“…Due to the high social and economic burden caused by vibriosis ( 2 ), together with the emergence of multidrug-resistant and highly virulent strains ( 3 , 4 ), it is imperative to gain more knowledge on the biology and genomics of Vibrio species in order to develop strategies to combat the pathogen. To date, only six complete genomes of V. harveyi strains are available (ATCC 33843, FDAARGOS_107, WXL538, 345, QT520, and 2011V-1164), and due to this sparsity of genomic data, species identification, comparative genomic analyses, or pathogenesis studies are challenging ( 5 , 6 ). Here, we report the complete genome sequence of V. harveyi strain ATCC 33866, which will contribute to the genetic data available of the Vibrio genus to facilitate further research.…”

Section: Announcementmentioning

confidence: 99%

Complete Genome Sequence of Vibrio harveyi Strain ATCC 33866

Yao

Zhou

Feng

et al. 2022

Microbiol Resour Announc

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Isolation of Harveyi clade Vibrio spp. collected in aquaculture farms: How can the identification issue be addressed?

Cited by 16 publications

References 60 publications

Diving into the unknown: identification of antimicrobial resistance hotspots in a tropical urban estuary

Diving into the unknown: identification of antimicrobial resistance hotspots in a tropical urban estuary

ANALISIS PERMODELAN DINAMIS KELIMPAHAN BAKTERI Vibrio sp. PADA BUDIDAYA UDANG VANAME, Litopenaeus vannamei

Complete Genome Sequence of Vibrio harveyi Strain ATCC 33866

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