Complete Genome Sequence of Vibrio harveyi Strain ATCC 33866

Yao, Yue; Zhou, Qianjin; Feng, Yu; Leptihn, Sebastian; Yu, Yunsong; Hua, Xiaoting

doi:10.1128/mra.00073-22

Cited by 2 publications

(1 citation statement)

References 9 publications

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“…All the equipment and culture media plates were disinfected by autoclaving at 115°C and 15 pressure for 20 min. Aeromonas hydrophila ATCC 7966 T (CP000462) and Vibrio harveyi ATCC 33866 (CP090179.1 and CP090178.1) (Seshadri et al, 2006; Yao et al, 2022) were used to test the antibacterial activity of BP‐AgNPs. A well‐diffusion assay was used to assess the efficacy of the biological process.…”

Section: Methodsmentioning

confidence: 99%

Plant based synthesis of silver nanoparticles, antimicrobial efficiency, and toxicological assessment using freshwater fish (Cyprinus carpio)

Khan,

Yousafzai

2023

Microscopy Res & Technique

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Silver nanoparticles (AgNPs) are widely used and have various applications, including medicine, electronics, and textiles. However, their increasing use raises concern about their potential environmental impact, particularly on aquatic organisms, such as fish, which are the primary consumers of aquatic environments and can be exposed to AgNPs through various routes. For this purpose, the leaves of the plant species Bellis perennis were used as a reductive agent to convert silver nitrate into AgNPs, to assess its toxicity against fish. Well‐dispersed and undersized AgNPs were obtained and confirmed using analytical techniques, including Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). Moreover, the AgNPs have shown significant antibacterial activity against Aeromonas hydrophila (25.71 ± 0.63) and Vibrio harveyi (22.39 ± 0.29). In addition, the toxicity of the obtained AgNPs was assessed by exposing Cyprinus carpio to various concentrations, including 0.06, 0.1, and 0.2 mg/L. The findings revealed that the AgNPs were significantly accumulated in the intestine, followed by the gills, liver, muscles, kidney, and brain. This bioaccumulation led to histological alterations and destruction in the villi of the intestine, regeneration of liver cells, and degeneration of the gill lamella.Research Highlights Plants based synthesis of AgNPs is mostly considered as eco‐friendly A significant antibacterial activity was obtained The plant mediated AgNPs were found less toxic The AgNPs was profoundly accumulated and causes histological alterations

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Section: Methodsmentioning

confidence: 99%

Plant based synthesis of silver nanoparticles, antimicrobial efficiency, and toxicological assessment using freshwater fish (Cyprinus carpio)

Khan,

Yousafzai

2023

Microscopy Res & Technique

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Improvements to the Rapid Detection of the Marine Pathogenic Bacterium, Vibrio harveyi, Using Loop-Mediated Isothermal Amplification (LAMP) in Combination with SYBR Green

2022

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The common methods that are presently used to identify Vibrio harveyi include microscopic examination and biochemical, immunological and PCR-based assays. These methods require technical expertise, and can be time-consuming. A rapid method is required for the high-throughput screening of large number of samples. As such, we have developed a rapid, simple yet sensitive and specific detection method based on the use of the loop-mediated isothermal amplification (LAMP) of DNA. A set of six primers, i.e., two outer, two inner and two loop primers, was designed based on the in silico analysis of a large pool of 39 strains of the toxR gene sequence of V. harveyi. The addition of the loop primers decreased the reaction time of the LAMP by more than half. Furthermore, with the application of SYBR Green, the result can be obtained as quickly as in 10 to 15 min without the need of gel electrophoresis. The specificity of the method primers was then determined by performing LAMP with Vibrio and non-Vibrio samples. LAMP has a greater sensitivity than PCR reaction. The sensitivity of PCR was at 0.6 pg concentration of V. harveyi recombinant plasmid DNA standard, while LAMP was able to detect lower amounts even at 0.6 fg. The development of the LAMP assay will provide a valuable tool for the high-throughput rapid detection of V. harveyi contamination both in laboratories and in the field.

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Complete Genome Sequence of Vibrio harveyi Strain ATCC 33866

Abstract: Here, we report the complete genome sequence of Vibrio harveyi strain ATCC 33866, generated from Illumina and Oxford Nanopore sequencing. The assembled genome sequence comprises two circular chromosomes with lengths of 3,504,760 bp and 2,218,060 bp, respectively.

Cited by 2 publications

References 9 publications

Plant based synthesis of silver nanoparticles, antimicrobial efficiency, and toxicological assessment using freshwater fish (Cyprinus carpio)

Plant based synthesis of silver nanoparticles, antimicrobial efficiency, and toxicological assessment using freshwater fish (Cyprinus carpio)

Improvements to the Rapid Detection of the Marine Pathogenic Bacterium, Vibrio harveyi, Using Loop-Mediated Isothermal Amplification (LAMP) in Combination with SYBR Green

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