Isolation of glioma cancer stem cells in relation to histological grades in glioma specimens

Kong, Byung Ho; Park, Na Ri; Shim, Jae Yong; Kim, Bo Kyung; Shin, Hye Jin; Lee, Ji Hyun; Huh, Yong Min; Lee, Su Jae; Kim, Se Hoon; Kim, Eui Hyun; Park, Eun Kyung; Chang, Jong Hee; Kim, Do Kyung; Kim, Sun Ho; Hong, Yong Kil; Kang, Seok‐Gu; Lang, Frederick F.

doi:10.1007/s00381-012-1964-9

Cited by 50 publications

(72 citation statements)

References 59 publications

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“…This is consistent with data in the literature which indicate that the ability of CSC to differentiate into oligodendrocytes is almost null [9] or very limited [12], [23], even when markers other than GalC have been considered [16]. On the contrary, both D2 and F11 cells expressed GFAP and βIII-Tubulin at high levels.…”

Section: Discussionsupporting

confidence: 91%

“…The histological heterogeneity of tumor is consistent with reports in the literature [18], [40]. It is interesting to note that tumors have arisen in the time range reported by other Authors [7], [12], [18] and that the concentration of NS cells required to induce them is 50-fold less than that required for LI cells. Moreover, tumors in xenografts have maintained the characteristics of the original cells about expression of Nestin, GFAP and βIII-Tubulin.…”

Section: Discussionsupporting

confidence: 90%

“…The markers generally used to evaluate the differentiation in neural stem cells are βIII-Tubulin, GFAP and GalC [9], [12], [16]–[18], [21]–[23].…”

Section: Discussionmentioning

confidence: 99%

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Isolation of Cancer Stem Cells from Three Human Glioblastoma Cell Lines: Characterization of Two Selected Clones

et al. 2014

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Cancer stem cells (CSC) were isolated via a non-adherent neurosphere assay from three glioma cell lines: LI, U87, and U373. Using a clonal assay, two clones (D2 and F11) were selected from spheres derived from LI cells and were characterized for the: expression of stem cell markers (CD133, Nestin, Musashi-1 and Sox2); proliferation; differentiation capability (determined by the expression of GalC, βIII-Tubulin and GFAP); Ca2+ signaling and tumorigenicity in nude mice. Both D2 and F11 clones expressed higher levels of all stem cell markers with respect to the parental cell line. Clones grew more slowly than LI cells with a two-fold increase in duplication time. Markers of differentiation (βIII-Tubulin and GFAP) were expressed at high levels in both LI cells and in neurospheres. The expression of Nestin, Sox2, and βIII-Tubulin was down-regulated in D2 and F11 when cultured in serum-containing medium, whereas Musashi-1 was increased. In this condition, duplication time of D2 and F11 increased without reaching that of LI cells. D2, F11 and parental cells did not express voltage-dependent Ca2+-channels but they exhibited increased intracellular Ca2+ levels in response to ATP. These Ca2+ signals were larger in LI cells and in spheres cultured in serum-containing medium, while they were smaller in serum-free medium. The ATP treatment did not affect cell proliferation. Both D2 and F11 induced the appearance of tumors when ortotopically injected in athymic nude mice at a density 50-fold lower than that of LI cells. All these data indicate that both clones have characteristics of CSC and share the same stemness properties. The findings regarding the expression of differentiation markers and Ca2+-channels show that both clones are unable to reach the terminal differentiation. Both D2 and F11 might represent a good model to improve the knowledge on CSC in glioblastoma and to identify new therapeutic approaches.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 90%

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Isolation of Cancer Stem Cells from Three Human Glioblastoma Cell Lines: Characterization of Two Selected Clones

et al. 2014

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“…After the holes were drilled on the mouse skull using a 26-gauge needle, GSC11-GFP cells (1 × 10 6 ) were implanted directly into right frontal lobe of nude mice using Hamilton syringe (Dongwoo Science Co., Seoul, Republic of Korea) at the depth of 4.5 mm. Cells were simultaneously injected into five mice using a multiple microinfusion syringe pump (Harvard Apparatus, Holliston, MA, USA) at a rate of 0.5 μl/min as previously described4748.…”

Section: Methodsmentioning

confidence: 99%

Terahertz reflectometry imaging for low and high grade gliomas

Kang

et al. 2016

Sci Rep

Self Cite

100

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Gross total resection (GTR) of glioma is critical for improving the survival rate of glioma patients. One of the greatest challenges for achieving GTR is the difficulty in discriminating low grade tumor or peritumor regions that have an intact blood brain barrier (BBB) from normal brain tissues and delineating glioma margins during surgery. Here we present a highly sensitive, label-free terahertz reflectometry imaging (TRI) that overcomes current key limitations for intraoperative detection of World Health Organization (WHO) grade II (low grade), and grade III and IV (high grade) gliomas. We demonstrate that TRI provides tumor discrimination and delineation of tumor margins in brain tissues with high sensitivity on the basis of Hematoxylin and eosin (H&E) stained image. TRI may help neurosurgeons to remove gliomas completely by providing visualization of tumor margins in WHO grade II, III, and IV gliomas without contrast agents, and hence, improve patient outcomes.

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“…Herewith the content of CSCs in glioma tissue increases in proportion to the increase of the malignancy degree with a parallel increase in the expression levels of immunocytochemical markers of their stem properties [4,[7][8][9]. It is generally recognized that the identification of CSCs in various tumor types, as well as in brain gliomas is associated with a poor prognosis of the disease [10][11][12][13][14].…”

Section: Abstract: Cancer Stem Cells Glioma Cell Culture Neurospherementioning

confidence: 99%

The biological properties of cancer stem cells in malignant gliomas in tissue culture: a review

Semenova¹,

Lisianyi²,

Rosumenko³

et al. 2018

JCOT

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Isolation of glioma cancer stem cells in relation to histological grades in glioma specimens

Abstract: This trend of stage-by-stage increase of gCSCs in gliomas showed statistical significance by chi-square test linear-by-linear association. We prove that the rates of existence of gCSCs increase proportionally as the WHO grades of gliomas rise.

Cited by 50 publications

References 59 publications

Isolation of Cancer Stem Cells from Three Human Glioblastoma Cell Lines: Characterization of Two Selected Clones

Isolation of Cancer Stem Cells from Three Human Glioblastoma Cell Lines: Characterization of Two Selected Clones

Terahertz reflectometry imaging for low and high grade gliomas

The biological properties of cancer stem cells in malignant gliomas in tissue culture: a review

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