Isolation of fucoidan from Sargassum polycystum brown algae: Structural characterization, in vitro antioxidant and anticancer activity

Palanisamy, Subramanian; Vinosha, Manoharan; Marudhupandi, Thangapandi; Rajasekar, Periyannan; Prabhu, Nagesh

doi:10.1016/j.ijbiomac.2017.03.182

Cited by 197 publications

(134 citation statements)

References 60 publications

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“…The signal at 6.25 ppm indicates α-anomeric protons of l-fucopyranosyl units [15]. The obtained results were similar to findings recorded from other brown seaweeds [3,[15][16][17]. HPAEC-PAD analysis of monosaccharide composition ( Figure 4B) proposed higher fucose (81.45%) and mannose (8.63%) content, which suggested that SHC4 was a mannofucan.…”

Section: Characterization Of Shc4 By Nmr and Monosaccharide Compositisupporting

confidence: 85%

“…The proton signals between 2.0-2.2 ppm represent alcoholic protons [16], while signals between 3.5-4.0 ppm were attributed to H2-H6 sugar residues. The prominent signal at 4.6 ppm, 1[H] can be attributed to protons at the anomeric center of 3-linked d-galactopyranosyl residues [16,17]. The signal at 6.25 ppm indicates α-anomeric protons of l-fucopyranosyl units [15].…”

Section: Characterization Of Shc4 By Nmr and Monosaccharide Compositimentioning

confidence: 99%

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Human Keratinocyte UVB-Protective Effects of a Low Molecular Weight Fucoidan from Sargassum horneri Purified by Step Gradient Ethanol Precipitation

et al. 2020

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Ultraviolet B (UVB) radiation-induced oxidative skin cell damage is a major cause of photoaging. In the present study, a low molecular weight fucoidan fraction (SHC4) was obtained from Sargassum horneri by Celluclast-assisted extraction, followed by step gradient ethanol precipitation. The protective effect of SHC4 was investigated in human keratinocytes against UVB-induced oxidative stress. The purified fucoidan was characterized by Fourier-transform infrared spectroscopy (FTIR), 1H nuclear magnetic resonance (NMR), agarose gel-based molecular weight analysis and monosaccharide composition analysis. SHC4 had a mean molecular weight of 60 kDa, with 37.43% fucose and 28.01 ± 0.50% sulfate content. The structure was mainly composed of α-L-Fucp-(1→4) linked fucose units. SHC4 treatment dose-dependently reduced intracellular reactive oxygen species (ROS) levels and increased the cell viability of UVB exposed HaCaT keratinocytes. Moreover, SHC4 dose-dependently inhibited UVB-induced apoptotic body formation, sub-G1 accumulation of cells and DNA damage. Inhibition of apoptosis was mediated via the mitochondria-mediated pathway, re-establishing the loss of mitochondrial membrane potential. The UVB protective effect of SHC4 was facilitated by enhancing intracellular antioxidant defense via nuclear factor erythroid 2–related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling. Further studies may promote the use of SHC4 as an active ingredient in cosmetics and nutricosmetics.

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Section: Characterization Of Shc4 By Nmr and Monosaccharide Compositisupporting

confidence: 85%

Section: Characterization Of Shc4 By Nmr and Monosaccharide Compositimentioning

confidence: 99%

Human Keratinocyte UVB-Protective Effects of a Low Molecular Weight Fucoidan from Sargassum horneri Purified by Step Gradient Ethanol Precipitation

et al. 2020

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“…Fucoidan is a fucose-rich sulfated polysaccharide found in various species of brown seaweed (4)(5)(6)(7). Fucoidan has been shown to exert various biological activities, including antioxidant, anti-inflammatory, anti-angiogenic, anti-coagulant, anti-bacterial, and anticancer effects (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18). Studies have shown anticancer activities of fucoidan in lung, breast, liver, colon, prostate, and bladder cancer cells (19)(20)(21)(22)(23).…”

Section: Introductionmentioning

confidence: 99%

Fucoidan downregulates insulin-like growth factor-I receptor levels in HT-29 human colon cancer cells

Kim

Nam

2018

Oncol Rep

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Fucoidan, a sulfated polysaccharide present in brown seaweed, has demonstrated anticancer activity in lung, breast, liver and colon cells. The insulin-like growth factor (IGF) signaling pathway regulates growth in HT-29 cells through the insulin receptor substrate-1 (IRS-1)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) and Ras/Raf/extracellular signal-regulated kinase (ERK) pathways. The aim of the present study was to investigate whether fucoidan downregulates the IGF-IR signaling pathway in HT-29 human colon cancer cells. Fucoidan treatment (0-1,000 µg/ml) was administered for 24 h in HT-29 cells. First, we investigated IRS-1/PI3K/AKT pathway-related protein expression levels following treatment with fucoidan (0-500 µg/ml) using western blot analysis. Fucoidan significantly inhibited the expression of IGF-IR, PTEN, PI3K and AKT as well as their phosphorylated forms (p-IRS-1, p-PI3K and p-AKT). Next, we investigated the effects of fucoidan on Ras/Raf/ERK pathway‑related protein expression levels in HT-29 cells. Fucoidan significantly inhibited the expression of IGF-IR, Shc, Ras, SOS, Raf and MEK. HT-29 cells were then incubated in the presence of fucoidan (0 or 250 µg/ml), and IGF-I (10 nM) was added for 0 to 60 min. Immunoprecipitation (IP) experiments showed that fucoidan inhibited IGF-I-induced phosphorylation of IGF-IR, PI3K, Shc (IP, IGF-IR), and phosphorylated IRS-1 and PI3K (IP, IRS-1) compared to the control group. Western blot analysis showed that fucoidan inhibited the expression of IGF-I-induced p-IGF-IR/IGF-IR and p-AKT/AKT, but not p-ERK/ERK. In conclusion, the inhibition of cell viability by fucoidan in HT-29 cells may be due to the downregulation of IGF-IR signaling through the main IRS-1/PI3K/AKT pathway. Fucoidan also partially impacted Ras/Raf signaling in the Ras/Raf/ERK pathway. Therefore, we suggest that fucoidan may be a suitable candidate chemopreventive agent in HT-29 colon cancer cells.

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“…pinnatifida [10], Fucus evanescens [11], Sargassum polycystum [12], Saccharina japonica [13], Himantothallus grandifolius [14] and Sargassum muticum [15] (reviewed in [16][17][18][19]) have also been investigated.…”

mentioning

confidence: 99%

Fucoidan Inhibition of Osteosarcoma Cells is Species and Molecular Weight Dependent

Gupta¹,

Silva²,

Radziun³

et al. 2019

Preprint

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Fucoidan is a brown algae-derived polysaccharide having several biomedical applications. This study simultaneously compares the anticancer activities of crude fucoidans from Fucus vesiculosus and Sargassum filipendula, and effects of low (LMW, 10-50kDa), medium (MMW, 50-100kDa) and high (HMW, >100kDa) molecular weight fractions of S. filipendula fucoidan against osteosarcoma cells. Glucose, fucose and acid levels were lower and sulphation was higher in F. vesiculosus crude fucoidan compared to S. filipendula crude fucoidan. MMW had highest the levels of sugars, acids and sulphation among molecular weight fractions. There was a dose dependent drop in focal adhesion formation and proliferation of cells for all fucoidan-types, but F. vesiculosus fucoidan and HMW had the strongest effects. G1-phase arrest was induced by F. vesiculosus fucoidan, MMW and HMW, however F. vesiculosus fucoidan treatment also caused accumulation in sub G1-phase. Mitochondrial damage occurred for all fucoidan-types, however F. vesiculosus fucoidan led to mitochondrial fragmentation. Annexin V/PI, TUNEL and cytochrome c staining confirmed stress induced apoptosis-like cell death for F. vesiculosus fucoidan but features of stress-induced necrosis-like cell death for S. filipendula fucoidans. There was also variation in penetrability of different fucoidans inside the cell. These differences in anti-cancer activity of fucoidans are applicable for osteosarcoma treatment.

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Isolation of fucoidan from Sargassum polycystum brown algae: Structural characterization, in vitro antioxidant and anticancer activity

Cited by 197 publications

References 60 publications

Human Keratinocyte UVB-Protective Effects of a Low Molecular Weight Fucoidan from Sargassum horneri Purified by Step Gradient Ethanol Precipitation

Human Keratinocyte UVB-Protective Effects of a Low Molecular Weight Fucoidan from Sargassum horneri Purified by Step Gradient Ethanol Precipitation

Fucoidan downregulates insulin-like growth factor-I receptor levels in HT-29 human colon cancer cells

Fucoidan Inhibition of Osteosarcoma Cells is Species and Molecular Weight Dependent

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