Human Keratinocyte UVB-Protective Effects of a Low Molecular Weight Fucoidan from Sargassum horneri Purified by Step Gradient Ethanol Precipitation

Fernando, Ilekuttige Priyan Shanura; Dias, Mawalle Kankanamge Hasitha Madhawa; Madusanka, Disanayaka Mudiyanselage Dinesh; Han, Eui Jeong; Kim, Min Ju; Jeon, You‐Jin; Lee, Kyounghoon; Cheong, Sun Hee; Han, Young Seok; Park, Sang Rul; Ahn, Ginnae

doi:10.3390/antiox9040340

Cited by 27 publications

(32 citation statements)

References 26 publications

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“…Fucoidan (FC) is found in brown seaweed and is a sulfated fucose-rich polysaccharide. A previous study indicated that FC has antioxidant, antitumor, anti-inflammatory, and anti-angiogenesis abilities [ 28 , 29 , 30 , 31 ], showing its potential for anti-endometriosis progression. However, the effects of FC on endometriosis are still unclear.…”

Section: Introductionmentioning

confidence: 99%

The Potential Effect of Fucoidan on Inhibiting Epithelial-to-Mesenchymal Transition, Proliferation, and Increase in Apoptosis for Endometriosis Treatment: In Vivo and In Vitro Study

et al. 2020

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Endometriosis is common in reproductive-age women and its pathology is to increase proliferation and migration to enhance epithelial-to-mesenchymal transition progression (EMT). However, treatments are currently limited, so it is important to explore new therapeutic drugs. Hence, in this study, we investigate the therapeutic effect of fucoidan (FC) on the progression and mechanisms of endometriosis. The cell viability of endometrial cell lines End1/E6E7 and Vk2/E6E7 treated with different concentrations of FC were assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell counting. Cell migration was evaluated using wound-healing assay. In an in vivo experiment, female Balb/c mice received surgically induced endometriosis followed by different concentrations of fucoidan for 6 weeks. High-frequency ultrasound imaging was applied to detect subsequent lesion growth. The results demonstrated that fucoidan inhibited the viability and migration ability of End1/E6E7 and Vk2/E6E7 cells. Additionally, the administration of fucoidan reduced the volume and weight of endometriotic lesions, decreased inflammatory cytokines and vascular endothelial growth factor (VEGF) of serum and lesions, and improved EMT proliferation and apoptosis-related protein expression. For the first time, fucoidan indicated anti-proliferative and anti-inflammatory effects as well as inhibited EMT progression and induced apoptosis, improving endometriosis.

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Section: Introductionmentioning

confidence: 99%

The Potential Effect of Fucoidan on Inhibiting Epithelial-to-Mesenchymal Transition, Proliferation, and Increase in Apoptosis for Endometriosis Treatment: In Vivo and In Vitro Study

et al. 2020

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“…Fucoidan from Sargassum horneri reduced ROS production and rescued the viability of UVB-exposed HaCaT cells by enhancing a Nrf2/HO-1 signaling pathway [ 144 ]. Fucoidan from Sargassum siliquastrum also increased Nrf2-mediated HO-1 expression and suppressed ROS production and mitochondria-mediated apoptosis in UVB-exposed HaCaT keratinocytes [ 145 ].…”

Section: Plant-derived Natural Nrf2 Modulators For Skin Protectionmentioning

confidence: 99%

“… Marine natural products activating the Nrf2-mediated pathway [ 139 , 140 , 141 , 142 , 143 , 144 , 145 ]. …”

Section: Figurementioning

confidence: 99%

Natural Nrf2 Modulators for Skin Protection

Boo

2020

Antioxidants

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Since the discovery of antioxidant responsive elements (ARE), which are commonly found in the promoter of the Phase II metabolism/antioxidant enzymes, and nuclear factor erythroid 2-related factor 2 (Nrf2), the transcription factor that binds to ARE, the study conducted in this field has expanded remarkably over the decades, and the Nrf2-mediated pathway is now recognized to occupy a central position in cell defense mechanisms. Induction of the Phase II metabolism/antioxidant enzymes through direct activation of Nrf2 can be a promising strategy for preventing degenerative diseases in general, but a dark side of this strategy should be considered, as Nrf2 activation can enhance the survival of cancer cells. In this review, we discuss the historical discovery of Nrf2 and the regulatory mechanism of the Nrf2-mediated pathway, focusing on the interacting proteins and post-translational modifications. In addition, we discuss the latest studies that examined various natural Nrf2 modulators for the protective roles in the skin, in consideration of their dermatological and cosmetic applications. Studies are reviewed in the order of time of research as much as possible, to help understand how and why such studies were conducted under the circumstances of that time. We hope that this review can serve as a steppingstone in conducting more advanced research by providing a scientific basis for researchers newly entering this field.

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“…Pretreatment with CTO in H 2 O 2 -treated cells regulated ROS production by inducing, expression of antioxidant enzymes (SOD, catalase, glutathione peroxidase (GSH-Px), glutathione reductase, and HO-1, and also prevented H 2 O 2 -induced apoptosis by regulating the expression of anti-apoptotic proteins, such as Bcl-2 and Bax. Environmental stress, involving oxidative stress, due to Ultraviolet (UV) and air pollutants contributing fine dust (FD) containing hazardous chemicals, induces triggering allergic reactions and inflammation of the skin, which lead to thickening of the epidermis, discoloration, skin wrinkling, loss of elasticity and skin-cell growth retardation [ 7 , 8 ]. Fernando et al [ 7 ] reported that a low molecular weight fucoidan fraction (SHC4, 60 kDa, with 37.43% fucose and 28.01% sulfate), isolated from Sargassum horneri , reduced intracellular ROS levels and increased the cell viability on UVB (280–320 nm) exposed HaCaT keratinocytes and inhibited UVB-induced apoptotic body formation, sub-G1 accumulation of cells through the mitochondria-mediated pathway.…”

mentioning

confidence: 99%

“…Environmental stress, involving oxidative stress, due to Ultraviolet (UV) and air pollutants contributing fine dust (FD) containing hazardous chemicals, induces triggering allergic reactions and inflammation of the skin, which lead to thickening of the epidermis, discoloration, skin wrinkling, loss of elasticity and skin-cell growth retardation [ 7 , 8 ]. Fernando et al [ 7 ] reported that a low molecular weight fucoidan fraction (SHC4, 60 kDa, with 37.43% fucose and 28.01% sulfate), isolated from Sargassum horneri , reduced intracellular ROS levels and increased the cell viability on UVB (280–320 nm) exposed HaCaT keratinocytes and inhibited UVB-induced apoptotic body formation, sub-G1 accumulation of cells through the mitochondria-mediated pathway. The UVB protective effect of SHC4 was facilitated by enhancing intracellular antioxidant defence via Nrf2-HO-1 signalling.…”

mentioning

confidence: 99%