Isolation of fermentative microbial isolates from sugar cane and beet molasses and evaluation for enhanced production of bioethanol

Hamouda, Hamed I.; Nassar, Hussein N.; Madian, Hekmat R.; Elsayed, Mohamed A.; El-Ghamry, A. A.

doi:10.1080/15567036.2015.1030050

Cited by 16 publications

(17 citation statements)

References 21 publications

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“…The microscopic characteristics included oval cells with budding. This is in agreement with the literature, since the same morphological characteristics were observed for yeasts isolated from sugar cane molasses (Hamouda et al, 2016), pineapple (Patil and Patil, 2010), and orange from the greater Mekong subregion (Techaparin et al, 2017). Indeed, it is well known that yeast is ubiquitous and can grow on different substrates (Tikka et al, 2013).…”

Section: Morphological Characterizationsupporting

confidence: 91%

“…Various microorganisms of indigenous strains capable of producing ethanol have been isolated from different local sources such as dates (Djelal et al, 2017), different fruits (Lee et al, 2011), cheese whey (Boudjema et al, 2016), sugar cane and beet molasses (Hamouda et al, 2016). However, strains isolated from the natural substrate gave much better performance than commercial strains (Djelal et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Identification of Yeast Strains From Sugarcane Molasses, Dates and Figs for Ethanol Production Under Conditions Simulating Algal Hydrolysate

Kechkar

Sayed

Cabrol

et al. 2019

Braz. J. Chem. Eng.

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Yeast strains were isolated from sugar cane molasses (S1), dates (S2) and figs (S3) and the ethanol production was evaluated in batch condition. A comparison was made with the yeast Saccharomyces cerevisiae. The strains showed tolerant characteristics to stressful conditions like salinity and ethanol. The isolated strains produced ethanol; at 20 h of fermentation ethanol yields were 0.38-0.39 g.g-1 , and the productivities were almost 0.58 g.L-1. S. cerevisiae and S1 tolerated up to 14% (v/v) of ethanol; while interestingly the isolates S2 and S3 were highly tolerant, up to 20% (v/v) ethanol. Thus, S2 and S3 could serve as potential strains for ethanol fermentation, with 0.27 and 0.29 g.g-1 yield of ethanol in the presence of 1.37 mol.L-1 NaCl. These values were higher than the value obtained using the yeast of reference and S1 (0.16 g.g-1). Co-cultures of S2 and S3 enhanced the ethanol production, increasing the yield of ethanol by 12.5% compared with the single culture. The strains were identified as species S.cerevisiae, and S2 and S3 were very similar. For an application in the valorization of biomass such as green macro-algae, some assays were done on a synthetic model medium of hydrolysate of macro-algae and the strains S2 and S3 demonstrated excellent fermentative performances.

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Section: Morphological Characterizationsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Yeast Strains From Sugarcane Molasses, Dates and Figs for Ethanol Production Under Conditions Simulating Algal Hydrolysate

Kechkar

Sayed

Cabrol

et al. 2019

Braz. J. Chem. Eng.

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“…[54]. Nevertheless, rice straw is highly heterogenous as compared to the combination of PKC and soybean pulp to support the growth of L. plantarum RI 11 [55]. Due to the abundance of glucose, sucrose and fructose [49][50][51][52][53], the cell population of L. plantarum RI 11 in M3 medium was the highest as reported by a previous study of Zajšek et al [56].…”

Section: Growth Profile Of L Plantarum Ri 11 In Media Supplemented With Renewable Natural Polymersmentioning

confidence: 86%

“…The cellulolytic and hemicellulolytic enzyme levels were comparably low in M1 medium. Hence, it can be concluded that though yeast extract was able to promote the growth of L. plantarum R1 11, but, due to the heterogeneity of the rice straw [55], a low level of cellulolytic and hemicellulolytic enzymes was secreted by the L. plantarum R1 11. Figure 4a-c demonstrate the significantly (p<0.05) lower specific extracellular endoglucanase, exoglucanase and β-glucosidase activities at pH 5, 6.5 and 8, respectively, when L. plantarum R1 11 was cultivated in basal medium supplemented with rice straw and soybean pulp (M2) medium.…”

Section: Extracellular Cellulolytic and Hemicellulolytic Enzyme Activities Of L Plantarum Ri 11mentioning

confidence: 99%

Enhancement of Versatile Extracellular Cellulolytic and Hemicellulolytic Enzyme Productions by Lactobacillus plantarum RI 11 Isolated from Malaysian Food Using Renewable Natural Polymers

et al. 2020

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Lactobacillus plantarum RI 11 was reported recently to be a potential lignocellulosic biomass degrader since it has the capability of producing versatile extracellular cellulolytic and hemicellulolytic enzymes. Thus, this study was conducted to evaluate further the effects of various renewable natural polymers on the growth and production of extracellular cellulolytic and hemicellulolytic enzymes by this novel isolate. Basal medium supplemented with molasses and yeast extract produced the highest cell biomass (log 10.51 CFU/mL) and extracellular endoglucanase (11.70 µg/min/mg), exoglucanase (9.99 µg/min/mg), β-glucosidase (10.43 nmol/min/mg), and mannanase (8.03 µg/min/mg), respectively. Subsequently, a statistical optimization approach was employed for the enhancement of cell biomass, and cellulolytic and hemicellulolytic enzyme productions. Basal medium that supplemented with glucose, molasses and soybean pulp (F5 medium) or with rice straw, yeast extract and soybean pulp (F6 medium) produced the highest cell population of log 11.76 CFU/mL, respectively. However, formulated F12 medium supplemented with glucose, molasses and palm kernel cake enhanced extracellular endoglucanase (4 folds), exoglucanase (2.6 folds) and mannanase (2.6 folds) specific activities significantly, indicating that the F12 medium could induce the highest production of extracellular cellulolytic and hemicellulolytic enzymes concomitantly. In conclusion, L. plantarum RI 11 is a promising and versatile bio-transformation agent for lignocellulolytic biomass.

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“…These adaptive responses require significant energy consumption and are non‐inheritable, resulting from the activation of specific stress‐related genes. Most of the yeast strains reported as intrinsically thermotolerant have been isolated from bioethanol production plants where they were exposed to high temperatures for long periods of time, but the genome‐wide characterization of these strains is poorly described (Amorim et al ., 2010; Abreu‐Cavalheiro and Monteiro, 2013; Hamouda et al ., 2016; Kechkar et al ., 2019). Based on adaptive laboratory evolution experiments, mutations that abrogated the expression of the C‐5 sterol desaturase Erg3, a non‐essential ergosterol biosynthetic enzyme, were found to lead to thermotolerance by replacement of the ‘flat‐structured’ ergosterol with the ‘bend‐structured’ fecosterol in the plasma membrane (Caspeta et al ., 2014).…”

Section: Introductionmentioning

confidence: 99%

The ABC transporter Pdr18 is required for yeast thermotolerance due to its role in ergosterol transport and plasma membrane properties

Godinho

Costa

Sá‐Correia

2020

Environmental Microbiology

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Among the mechanisms by which yeast overcomes multiple stresses is the expression of genes encoding ATP-binding cassette (ABC) transporters required for resistance to a wide range of toxic compounds. These substrates may include weak acids, alcohols, agricultural pesticides, polyamines, metal cations, as in the case of Pdr18. This pleotropic drug resistance transporter was previously proposed to transport ergosterol at the plasma membrane (PM) level contributing to the maintenance of PM lipid organization and reduced diffusional permeation induced by lipophilic compounds. The present work reports a novel phenotype associated with the putative drug/xenobiotic-efflux-pump transporter Pdr18: the resistance to heat shock and to long-term growth at supra-optimal temperatures. Cultivation at 40 C was demonstrated to lead to higher PM permeabilization of a pdr18Δ cell population with the PDR18 gene deleted compared with the parental strain population, as indicated by flow cytometry analysis of propidium iodide stained cells. Cells of pdr18Δ grown at 40 C also exhibited increased transcription levels from genes of the ergosterol biosynthetic pathway, compared with parental cells. However, this adaptive response at 40 C was not enough to maintain PM physiological ergosterol levels in the population lacking the Pdr18 transporter and free ergosterol precursors accumulate in the deletion mutant cells.

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Isolation of fermentative microbial isolates from sugar cane and beet molasses and evaluation for enhanced production of bioethanol

Cited by 16 publications

References 21 publications

Isolation and Identification of Yeast Strains From Sugarcane Molasses, Dates and Figs for Ethanol Production Under Conditions Simulating Algal Hydrolysate

Isolation and Identification of Yeast Strains From Sugarcane Molasses, Dates and Figs for Ethanol Production Under Conditions Simulating Algal Hydrolysate

Enhancement of Versatile Extracellular Cellulolytic and Hemicellulolytic Enzyme Productions by Lactobacillus plantarum RI 11 Isolated from Malaysian Food Using Renewable Natural Polymers

The ABC transporter Pdr18 is required for yeast thermotolerance due to its role in ergosterol transport and plasma membrane properties

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