Enhancement of Versatile Extracellular Cellulolytic and Hemicellulolytic Enzyme Productions by Lactobacillus plantarum RI 11 Isolated from Malaysian Food Using Renewable Natural Polymers

Abstract

Lactobacillus plantarum RI 11 was reported recently to be a potential lignocellulosic biomass degrader since it has the capability of producing versatile extracellular cellulolytic and hemicellulolytic enzymes. Thus, this study was conducted to evaluate further the effects of various renewable natural polymers on the growth and production of extracellular cellulolytic and hemicellulolytic enzymes by this novel isolate. Basal medium supplemented with molasses and yeast extract produced the highest cell biomass (log 10.51 CFU/mL) and extracellular endoglucanase (11.70 µg/min/mg), exoglucanase (9.99 µg/min/mg), β-glucosidase (10.43 nmol/min/mg), and mannanase (8.03 µg/min/mg), respectively. Subsequently, a statistical optimization approach was employed for the enhancement of cell biomass, and cellulolytic and hemicellulolytic enzyme productions. Basal medium that supplemented with glucose, molasses and soybean pulp (F5 medium) or with rice straw, yeast extract and soybean pulp (F6 medium) produced the highest cell population of log 11.76 CFU/mL, respectively. However, formulated F12 medium supplemented with glucose, molasses and palm kernel cake enhanced extracellular endoglucanase (4 folds), exoglucanase (2.6 folds) and mannanase (2.6 folds) specific activities significantly, indicating that the F12 medium could induce the highest production of extracellular cellulolytic and hemicellulolytic enzymes concomitantly. In conclusion, L. plantarum RI 11 is a promising and versatile bio-transformation agent for lignocellulolytic biomass.

Comparative Studies of Inhibitory and Antioxidant Activities, and Organic Acids Compositions of Postbiotics Produced by Probiotic Lactiplantibacillus plantarum Strains Isolated From Malaysian Foods

“…The active L. plantarum strains were washed once with sterile 0.85% (w/v) NaCl (Merck, Darmstadt, Germany) solution and adjusted to 10 9 CFU/mL to be used as a 10% (v/v) inoculum according to the method described by Mohamad Zabidi et al ( 38 ). The postbiotics of the six strains of L. plantarum were prepared at 30°C for 24 h according to the method described by Kareem et al ( 22 ) by using MRS as a control medium and their respective formulated media as shown in Table 1 .…”

Lactiplantibacillus plantarum Postbiotics: Alternative of Antibiotic Growth Promoter to Ameliorate Gut Health in Broiler Chickens

“…Postbiotics are the bioactive secondary metabolites produced from lactic acid bacteria during growth and metabolism, which has similar positive effects to probiotics but without the inclusion of bacterial cells ( 32 , 33 ). The postbiotic produced from L. plantarum comprised many beneficial compounds such as antioxidant compounds, organic acids, bacteriocins, and enzymes ( 16 , 20 ). As a result, the postbiotics modulate the immune response, fight against pathogenic infections, stimulate the proliferation of good bacteria in the gut, and improve livestock growth performance and production ( 7 , 14 , 17 ).…”

“…The stock cultures kept at −20°C were revived twice using de-Mann Rogosa Sharpe (MRS) broth and incubated at 30°C for 48 and 24 h as described by Foo et al ( 14 ). Then, the active bacteria were washed once with sterile 0.85% (w/v) NaCl (Merck, Darmstadt, Germany) solution and adjusted to 10 9 CFU/ml before being inoculated into formulated media and incubated at 30°C for 24 h according to the method described by Mohamad Zabidi et al ( 20 ). Finally, the postbiotic produced was ready to be used after centrifuging at 10,000 g for 15 min at 4°C and filtered through 0.22-μm cellulose acetate membrane (Sartorius Minisart, Germany) to remove all the viable bacterial cells.…”

Lactic acid bacteria: an essential probiotic and starter culture for the production of yoghurt

“…Bacteria like any living organism require a suitable biophysical and biochemical habitat and depend largely on available nutrients in their environment to support their existence, growth and cellular biosynthesis (Hayek et al ., 2019; Mohamad Zabidi et al ., 2020). The general knowledge of nutritional requirements associated with most bacteria thus ensures flexibility, when formulating growth media for specific strains in the laboratory.…”

“…Carbon sources such as sugars, amino acids and fats are critical for optimising the growth potential of bacteria (Hayek & Ibrahim, 2013). It is noteworthy that enzyme‐producing microbes are dependent on glucose as their predominant carbon source, while nitrogen sources of tryptone and peptone efficiently support the growth of bacteria (Mohamad Zabidi et al ., 2020).…”