Isolation of endothelial progenitor cells from human adipose tissue

Abstract

Adipose tissue is a rich source of stem cells, especially mesenchymal stem cells (MSCs). This study aimed to identify and isolate endothelial progenitor cells (EPCs) from human adipose tissue. Belly adipose tissues were collected from donors with consent. Stromal vascular fractions (SVFs) were extracted from adipose tissues by enzyme collagenase using commercial kits. SVFs were cultured in MSCCult medium for 24 h to obtain MSCs, then supernatant was collected and cell pellet cultured in EGM-2 medium to obtain adipose tissue EPCs (ADEPCs). ADEPCs were checked for surface marker expression of CD31 and VEGFR2, and for angiogenesis capability in vitro. The results showed that SVFs contained a pool of EPCs with strong angiogenesis potential and that adipose tissue is not only a source for MSCs but also for EPCs. Therefore, ADEPCs may a useful source of EPCs for vascular medicine.

Vascular tissue engineering from human adipose tissue: fundamental phenotype of its resident microvascular endothelial cells and stromal/stem cells

“…Adipose tissue is an attractive source of cells for vascular tissue engineering because it can be harvested in large quantities with minimally invasive procedures [ 1 , 2 ]. Despite its intrinsic ECs [17] , [18] , [19] , [20] , [21] , considerable effort is directed at deriving endothelium from its resident stem and progenitor cells [5] , [6] , [7] , [8] , [9] , [10] , [11] , [12] , [13] , [14] , [15] , [16] . Here, we demonstrate that the utility of ASCs for vascular tissue engineering resides not in their phenotypic plasticity, but in their perivascular phenotype and capacity to support HAMVECs.…”

“…Endothelial cells (ECs) are consequently indispensable for the construction of vasculature. Remarkably, effort continues to be primarily directed at deriving endothelium from adipose tissue-resident stem and progenitor cells [5] , [6] , [7] , [8] , [9] , [10] , [11] , [12] , [13] , [14] , [15] , [16] , despite the existence of natively differentiated ECs within its stromal vascular fraction [17] , [18] , [19] , [20] , [21] .…”

Differential phenotype and behavior in culture of CD34 positive cells from peripheral blood and adipose tissue

“…Adipose tissue has been found to be an abundant source of progenitor cells [ 18 , 20 ]. Pham et al suggested the existence of a distinct EPC phenotype within adipose tissue [ 17 , 19 ]. Where the stromal vascular fraction is isolated from adipose tissue and placed in culture, rapid early appearance of endothelial cells has been noted by our group and by others [ 20 , 23 , 24 , 25 ].…”

“…In recent years, great interest has developed in adipose tissue (AT) as a source of stem and progenitor cells [ 16 ], and there is substantial evidence for the stromal vascular fraction (SVF) from AT to yield cells capable of endothelial differentiation, and to promote angiogenesis in a hindlimb ischemia model [ 17 , 18 , 19 , 20 ]. These findings are supportive of the hypothesis that adipose tissue may be a source of true endothelial progenitor cells.…”

Vascular Regeneration for Ischemic Retinopathies: Hope from Cell Therapies

“…44 EPCs have been isolated from cord blood 45 , peripheral blood 46 , bone marrow 47 , placenta 48 , lungs 49 and adipose tissue. 50 The so-called "putative" EPCs were derived from the mononuclear cell fraction of blood 51 and from sorted CD34 + cells cultured on fibronectin. 52 These 'EPCs' were found to express CD31 and vascular endothelial growth factor receptor 2 (VEGFR2).…”