Isolation of endothelial progenitor cells from human adipose tissue

Pham, Phuc Van; Vu, Ngoc Bich; Nguyen, Hoa; Phan, Ngoc Kim

doi:10.7603/s40730-016-0024-6

Cited by 3 publications

(3 citation statements)

References 47 publications

(20 reference statements)

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“…The study underscores the feasibility of isolating EPCs from AT by selecting slowly adherent cells from SVFs. These findings highlight the potential and rationale for utilizing AT in EPC isolation for the treatment of vascular diseases [118]. It is crucial to note that EPCs derived from AT exhibit greater proliferative capacity compared to EPCs sourced directly from ADSCs [119].…”

Section: Endothelial Cells Differentiationmentioning

confidence: 89%

“…EPCs have proven effective in treating stroke, hindlimb ischemia, myocardial infarction, and diabetic foot ulcers [114][115][116][117]. However, the primary challenge associated with EPCbased transplantation lies in the limited quantity of these cells [118]. EPCs, sourced from umbilical cord blood, bone marrow, or peripheral blood, are scarce.…”

Section: Endothelial Cells Differentiationmentioning

confidence: 99%

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Adipose-Derived Stem Cells: Angiogenetic Potential and Utility in Tissue Engineering

Biniazan,

Stoian,

Haykal

2024

IJMS

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Adipose tissue (AT) is a large and important energy storage organ as well as an endocrine organ with a critical role in many processes. Additionally, AT is an enormous and easily accessible source of multipotent cell types used in our day for all types of tissue regeneration. The ability of adipose-derived stem cells (ADSCs) to differentiate into other types of cells, such as endothelial cells (ECs), vascular smooth muscle cells, or cardiomyocytes, is used in tissue engineering in order to promote/stimulate the process of angiogenesis. Being a key for future successful clinical applications, functional vascular networks in engineered tissue are targeted by numerous in vivo and ex vivo studies. The article reviews the angiogenic potential of ADSCs and explores their capacity in the field of tissue engineering (TE).

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Section: Endothelial Cells Differentiationmentioning

confidence: 89%

Section: Endothelial Cells Differentiationmentioning

confidence: 99%

Adipose-Derived Stem Cells: Angiogenetic Potential and Utility in Tissue Engineering

Biniazan,

Stoian,

Haykal

2024

IJMS

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“…Endothelial progenitor cells (EPCs) with high angiogenic potential can be isolated easily from SVF ( Van Pham et al, 2016 ) and used further for bioengineering purposes. It is important to mention that adipose tissue-derived EPCs demonstrate higher proliferative potential than EPCs obtained from ASCs themselves ( Zhou et al, 2015 ).…”

Section: Pro-angiogenic Activity Within Adipose Clonogenic Nichesmentioning

confidence: 99%

Plasticity of Adipose Tissue-Derived Stem Cells and Regulation of Angiogenesis

et al. 2018

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Adipose tissue is recognized as an important organ with metabolic, regulatory, and plastic roles. Adipose tissue-derived stem cells (ASCs) with self-renewal properties localize in the stromal vascular fraction (SVF) being present in a vascular niche, thereby, contributing to local regulation of angiogenesis and vessel remodeling. In the past decades, ASCs have attracted much attention from biologists and bioengineers, particularly, because of their multilineage differentiation potential, strong proliferation, and migration abilities in vitro and high resistance to oxidative stress and senescence. Current data suggest that the SVF serves as an important source of endothelial progenitors, endothelial cells, and pericytes, thereby, contributing to vessel remodeling and growth. In addition, ASCs demonstrate intriguing metabolic and interlineage plasticity, which makes them good candidates for creating regenerative therapeutic protocols, in vitro tissue models and microphysiological systems, and tissue-on-chip devices for diagnostic and regeneration-supporting purposes. This review covers recent achievements in understanding the metabolic activity within the SVF niches (lactate and NAD+ metabolism), which is critical for maintaining the pool of ASCs, and discloses their pro-angiogenic potential, particularly, in the complex therapy of cardiovascular and cerebrovascular diseases.

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Endothelial progenitor cells for fabrication of engineered vascular units and angiogenesis induction

Rashidi,

Bagherpour,

Abbasi‐Malati

et al. 2024

Cell Proliferation

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The promotion of vascularization and angiogenesis in the grafts is a crucial phenomenon in the healing process and tissue engineering. It has been shown that stem cells, especially endothelial progenitor cells (EPCs), can stimulate blood vessel formation inside the engineered hydrogels after being transplanted into the target sites. The incorporation of EPCs into the hydrogel can last the retention time, long‐term survival, on‐target delivery effects, migration and differentiation into mature endothelial cells. Despite these advantages, further modifications are mandatory to increase the dynamic growth and angiogenesis potential of EPCs in in vitro and in vivo conditions. Chemical modifications of distinct composites with distinct physical properties can yield better regenerative potential and angiogenesis during several pathologies. Here, we aimed to collect recent findings related to the application of EPCs in engineered vascular grafts and/or hydrogels for improving vascularization in the grafts. Data from the present article can help us in the application of EPCs as valid cell sources in the tissue engineering of several ischemic tissues.

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Isolation of endothelial progenitor cells from human adipose tissue

Cited by 3 publications

References 47 publications

Adipose-Derived Stem Cells: Angiogenetic Potential and Utility in Tissue Engineering

Adipose-Derived Stem Cells: Angiogenetic Potential and Utility in Tissue Engineering

Plasticity of Adipose Tissue-Derived Stem Cells and Regulation of Angiogenesis

Endothelial progenitor cells for fabrication of engineered vascular units and angiogenesis induction

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