Adipose-Derived Stem Cells: Angiogenetic Potential and Utility in Tissue Engineering

Biniazan, Felor; Stoian, Alina; Haykal, Siba

doi:10.3390/ijms25042356

IJMS

2024

DOI: 10.3390/ijms25042356

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Adipose-Derived Stem Cells: Angiogenetic Potential and Utility in Tissue Engineering

Felor Biniazan,

Alina Stoian,

Siba Haykal

Abstract: Adipose tissue (AT) is a large and important energy storage organ as well as an endocrine organ with a critical role in many processes. Additionally, AT is an enormous and easily accessible source of multipotent cell types used in our day for all types of tissue regeneration. The ability of adipose-derived stem cells (ADSCs) to differentiate into other types of cells, such as endothelial cells (ECs), vascular smooth muscle cells, or cardiomyocytes, is used in tissue engineering in order to promote/stimulate th… Show more

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2024

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Cited by 6 publications

References 201 publications

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Effects of Low‐Intensity Pulsed Ultrasound on the Regulation of Free Fatty Acid Release in 3T3‐L1 Cells

Wu,

Xiao,

Deng

et al. 2024

J of Ultrasound Medicine

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ObjectivesTo investigate the effects of low‐intensity pulsed ultrasound (LIPUS) on the proliferation, differentiation, and tumor necrosis factor‐α (TNF‐α)‐induced lipolysis of 3T3‐L1 cells, and to explore the feasibility of regulating the release of free fatty acids (FFA) to prevent lipotoxicity.MethodsDifferent intensities (30, 60, 90, and 120 mW/cm2) of LIPUS were applied to 3T3‐L1 preadipocytes for different durations (5, 10, 15, 20, 25, and 30 minutes). Appropriate parameters for subsequent experiments were selected by assessing cell viability. The effect of LIPUS on the proliferation and differentiation of 3T3‐L1 cells was evaluated by microscope observation, flow cytometry, and lipid content determination. After treated with LIPUS and TNF‐α (50 ng/mL), the degree of lipolysis was assessed by measuring the extracellular FFA content. Quantitative real‐time polymerase chain reaction (qRT‐PCR) was used to detect the mRNA expression of relevant genes.ResultsDifferent parameters of LIPUS significantly enhance the viability of 3T3‐L1 cells (P < .05), with 20 minutes and 30 mW/cm2 as the most suitable settings. After LIPUS treatment, 3T3‐L1 cell proliferation accelerated, apoptosis rate and G1 phase cell proportion decreased, the content of lipid droplets and TG was increased in differentiated cells, while FFA release decreased (P < .05). The expression of PCNA, PPARγ, C/EBPα, Perilipin A mRNA increased, and the expression of TNF‐α, ATGL, HSL mRNA decreased (P < .05).ConclusionsLIPUS could promote the proliferation and differentiation of 3T3‐L1 cells and inhibit TNF‐α‐induced lipolysis, indicating its potential as a therapy for mitigating lipotoxicity caused by decompensated adipocytes.

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Effects of Low‐Intensity Pulsed Ultrasound on the Regulation of Free Fatty Acid Release in 3T3‐L1 Cells

Wu,

Xiao,

Deng

et al. 2024

J of Ultrasound Medicine

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Breathing new life into tissue engineering: exploring cutting-edge vascularization strategies for skin substitutes

Iqbal,

Riaz,

Biedermann

et al. 2024

Angiogenesis

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Tissue-engineered skin substitutes (TESS) emerged as a new therapeutic option to improve skin transplantation. However, establishing an adequate and rapid vascularization in TESS is a critical factor for their clinical application and successful engraftment in patients. Therefore, several methods have been applied to improve the vascularization of skin substitutes including (i) modifying the structural and physicochemical properties of dermal scaffolds; (ii) activating biological scaffolds with growth factor-releasing systems or gene vectors; and (iii) developing prevascularized skin substitutes by loading scaffolds with capillary-forming cells. This review provides a detailed overview of the most recent and important developments in the vascularization strategies for skin substitutes. On the one hand, we present cell-based approaches using stem cells, microvascular fragments, adipose tissue derived stromal vascular fraction, endothelial cells derived from blood and skin as well as other pro-angiogenic stimulation methods. On the other hand, we discuss how distinct 3D bioprinting techniques and microfluidics, miRNA manipulation, cell sheet engineering and photosynthetic scaffolds like GelMA, can enhance skin vascularization for clinical applications. Finally, we summarize and discuss the challenges and prospects of the currently available vascularization techniques that may serve as a steppingstone to a mainstream application of skin tissue engineering. Graphical abstract

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Current progress and limitations of research regarding the therapeutic use of adipose-derived stem cells: literature review

Skrypnyk

2024

J.Umm Al-Qura Univ. Appll. Sci.

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Background Adipose tissue has recently become one of the most promising and predominant sources of mesenchymal stem cells owing to its high accessibility, culturing properties, regenerative potential, and relatively fewer ethical considerations. From the time of the adipose-derived stem cells (ADSCs) discovery, many beneficial properties have been found, including their regenerative, anti-inflammatory, immunomodulatory, and antimicrobial effects. The number of publications and clinical trials using ADSCs has increased significantly worldwide, attesting to the promising nature of the therapeutic properties of ADSCs. Main body of the abstract In clinical studies, ADSCs are mainly used to treat wounds, multiple sclerosis, soft tissue trauma, aging, diabetes, Parkinson’s disease, bone and cartilage regeneration, strokes, and spinal cord injuries. Few and insignificant adverse effects after ADSC treatment have been documented, suggesting their relative safety for clinical use. Despite significant progress in ADSC-related studies, several issues are yet to be addressed, including a lack of standardization of ADSC-associated protocols and the methods used to obtain them, inconsistent dosages, small numbers of patients in each treatment group, and variable graft purity. This severely complicates our ability to compare these studies, making the results even of similar studies controversial. Short conclusion This review described the current stage of ADSCs-based treatment outcomes and their limitations, associated with standardization of ADSCs.

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Adipose-Derived Stem Cells: Angiogenetic Potential and Utility in Tissue Engineering

Cited by 6 publications

References 201 publications

Effects of Low‐Intensity Pulsed Ultrasound on the Regulation of Free Fatty Acid Release in 3T3‐L1 Cells

Effects of Low‐Intensity Pulsed Ultrasound on the Regulation of Free Fatty Acid Release in 3T3‐L1 Cells

Breathing new life into tissue engineering: exploring cutting-edge vascularization strategies for skin substitutes

Current progress and limitations of research regarding the therapeutic use of adipose-derived stem cells: literature review

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