Isolation of endophytic diazotrophic bacteria from wetland rice

Barraquio, Wilfredo L.; Revilla, L.; Ladha, J. K.

doi:10.1007/978-94-011-5744-5_3

Cited by 59 publications

(83 citation statements)

References 35 publications

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“…Production of enzymes, such as endoglucanases [35] and endopolygalacturonidases [25], seems to be indispensable in this process. At this point, competent endophytes can quickly multiply inside the plant [46,47], often reaching high cell numbers [48]. rhizospheric colonizers, once they are established within the tissues of the host plant, are no longer subject to the variations of changing soil conditions.…”

Section: Introductionmentioning

confidence: 99%

Potential of Bacterial Endophytes as Plant Growth Promoting Factors

Chaturvedi¹,

Singh²,

Gupta³

2016

J Plant Pathol Microbiol

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Section: Introductionmentioning

confidence: 99%

Potential of Bacterial Endophytes as Plant Growth Promoting Factors

Chaturvedi¹,

Singh²,

Gupta³

2016

J Plant Pathol Microbiol

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“…mycotoxins, altechromones and flavonoids) which are required for the normal functioning of plants under adverse conditions (Strobel 2003). Diazotrophic bacterial and actinomycete populations of rice are reported to promote plant growth promotion and to possess the potential for nitrogen fixation and disease resistance (Barraquio et al 1997, Tian et al 2004. Fungal endophytes also have been detected in cultivated rice and they have shown to possess antagonistic or plant growth stimulating properties (Yuan et al 2009, Naik et al 2009).…”

Section: Introductionmentioning

confidence: 99%

An inoculum of endophytic fungi for improved growth of a traditional ricevariety in Sri Lanka

Wijesooriya¹,

Deshappriya²

2016

Trop. Plant Res.

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Use of chemical fertilizers and pesticides in rice cultivation has incurred many environmental and health problems in Sri Lanka. Therefore, there is renewed interest in cultivating traditional rice varieties as they are more amenable to organic farming practices. However, as the yield of these varieties is comparatively low, strategies to enhance their performance should be investigated. As endophytes of plants are reported to promote growth and yield of a number of crop varieties, this study was aimed at studying the endophytic fungal assemblage present in the traditional rice variety Kuruluthuda with a view to evaluate their capacity to enhance plant growth and yield. Twenty seven endophytic fungal species were isolated from different parts of Kuruluthuda rice plants collected from a paddy field cultivated using organic fertilizers in the Gampaha district, Sri Lanka. Two frequently isolated endophytic fungal spp. i.e. Acremonium and Arthrobotrys (frequencies of isolation 60% and 38.6% respectively) were introduced separately and in combination torice seedlings using spore suspension and plate methods to determine their effect on growth and yield under green house and field conditions. All endophyte inoculated plants showed a significant difference (P ≤ 0.05) in plant growth (height, fresh weight and dry weight), number of tillers and yield when compared withnon-inoculated plant sunder both green house and field conditions. The effect of Acremonium and Arthrobotrys when introduced in combination showed a significant difference (P ≤ 0.05) in the fresh weight, dry weight, tiller number and yield (weight of seeds harvested) when compared to their individual effects under field conditions which indicates that the two endophytes in combination can be used as a better inoculum to improve biomass and yield of the plants of rice variety tested. This is the first report of the endophytic mycoflora of the rice variety Kuruluthuda and their potential use for growth promotion and yield enhancement.

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“…Wild rice species are likely to harbor unique populations of nitrogen-fixing bacteria that differ from those in extensively bred modern varieties of cultivated rice (10). Nitrogen-fixing endophytes in rice were reported to be higher in stems than in roots, indicating that rice stems probably provide a suitable niche (5). Additionally, use of rice stems for isolation of diazotrophic bacteria can minimize the possibility of contamination from soil, because the rice stems are completely covered with successive leaf sheaths before heading (14).…”

mentioning

confidence: 99%

Endophytic Colonization and In Planta Nitrogen Fixation by a Herbaspirillum sp. Isolated from Wild Rice Species

El-Beltagy

Nishioka

Satô

et al. 2001

Appl Environ Microbiol

421

302

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Nitrogen-fixing bacteria were isolated from the stems of wild and cultivated rice on a modified Rennie medium. Based on 16S ribosomal DNA (rDNA) sequences, the diazotrophic isolates were phylogenetically close to four genera: Herbaspirillum, Ideonella, Enterobacter, and Azospirillum. Phenotypic properties and signature sequences of 16S rDNA indicated that three isolates (B65, B501, and B512) belong to the Herbaspirillum genus. To examine whether Herbaspirillum sp. strain B501 isolated from wild rice, Oryza officinalis, endophytically colonizes rice plants, the gfp gene encoding green fluorescent protein (GFP) was introduced into the bacteria. Observations by fluorescence stereomicroscopy showed that the GFP-tagged bacteria colonized shoots and seeds of aseptically grown seedlings of the original wild rice after inoculation of the seeds. Conversely, for cultivated rice Oryza sativa, no GFP fluorescence was observed for shoots and only weak signals were observed for seeds. Observations by fluorescence and electron microscopy revealed that Herbaspirillum sp. strain B501 colonized mainly intercellular spaces in the leaves of wild rice. Colony counts of surface-sterilized rice seedlings inoculated with the GFP-tagged bacteria indicated significantly more bacterial populations inside the original wild rice than in cultivated rice varieties. Moreover, after bacterial inoculation, in planta nitrogen fixation in young seedlings of wild rice, O. officinalis, was detected by the acetylene reduction and 15 N 2 gas incorporation assays. Therefore, we conclude that Herbaspirillum sp. strain B501 is a diazotrophic endophyte compatible with wild rice, particularly O. officinalis.

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Isolation of endophytic diazotrophic bacteria from wetland rice

Cited by 59 publications

References 35 publications

Potential of Bacterial Endophytes as Plant Growth Promoting Factors

Potential of Bacterial Endophytes as Plant Growth Promoting Factors

An inoculum of endophytic fungi for improved growth of a traditional ricevariety in Sri Lanka

Endophytic Colonization and In Planta Nitrogen Fixation by a Herbaspirillum sp. Isolated from Wild Rice Species

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