Nitrogen-fixing bacteria were isolated from the stems of wild and cultivated rice on a modified Rennie medium. Based on 16S ribosomal DNA (rDNA) sequences, the diazotrophic isolates were phylogenetically close to four genera: Herbaspirillum, Ideonella, Enterobacter, and Azospirillum. Phenotypic properties and signature sequences of 16S rDNA indicated that three isolates (B65, B501, and B512) belong to the Herbaspirillum genus. To examine whether Herbaspirillum sp. strain B501 isolated from wild rice, Oryza officinalis, endophytically colonizes rice plants, the gfp gene encoding green fluorescent protein (GFP) was introduced into the bacteria. Observations by fluorescence stereomicroscopy showed that the GFP-tagged bacteria colonized shoots and seeds of aseptically grown seedlings of the original wild rice after inoculation of the seeds. Conversely, for cultivated rice Oryza sativa, no GFP fluorescence was observed for shoots and only weak signals were observed for seeds. Observations by fluorescence and electron microscopy revealed that Herbaspirillum sp. strain B501 colonized mainly intercellular spaces in the leaves of wild rice. Colony counts of surface-sterilized rice seedlings inoculated with the GFP-tagged bacteria indicated significantly more bacterial populations inside the original wild rice than in cultivated rice varieties. Moreover, after bacterial inoculation, in planta nitrogen fixation in young seedlings of wild rice, O. officinalis, was detected by the acetylene reduction and 15 N 2 gas incorporation assays. Therefore, we conclude that Herbaspirillum sp. strain B501 is a diazotrophic endophyte compatible with wild rice, particularly O. officinalis.
SUMMARYConditions of drought or waterlogging lead to greatly increased internal ethylene concentrations in Vida faba L. These increases appear to be due partly to decreased diffusion and partly to increased synthesis and are superimposed on normal diurnal fluctuations in internal ethylene concentration. The higher concentrations observed are correlated with a reduction in growth rate and increased leaf and flower abscission and senescence. The role of ethylene in the mediation of developmental responses to water stress is discussed.
Abstract The planet earth, on which we live in communities, is being increasingly 'ruptured' because of human activities; its carrying capacity is under great stress because of demographic pressures. The pressure is especially affecting the people living in the dry areas because of the marginal and fragile nature of the resources they have access to. There are over 2,000 million hectares of land that have been degraded, with a loss of agrobiodiversity, increased water scarcity and increased natural resource destruction. Superimposed on this is the fact that the neglectful and exploitive use of natural resources has set the train of global climate change in motion. It is anticipated that the impact of climate change will cut across all boundaries. Crops, cropping systems, rotations and biota will undergo transformation. To maintain the balance in the system, there is a need for new knowledge, alternative policies and institutional changes. The marginalized people in dry areas are likely to be most seriously hit by the shifts in moisture and temperature regimes as a result of the global climate change. To help them cope with the challenges, there is a need for a new paradigm in agricultural research and technology transfer that makes full use of modern science and technology in conjunction with traditional knowledge. This necessitates more investment by international agencies and national governments for supporting the relevant integrated research and sustainable development efforts, with full participation of the target communities. Only such an approach can enable the vulnerable communities of the dryland areas to use the natural resources in a sustainable manner and thus help protect the environment for future generations. The clock is ticking and the future of the world lies in the collective responsibility and wisdom of all nations on this planet. This should be reflected in the endorsement of a solid future plan.
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