Isolation of Desulfomicrobium orale sp. nov. and Desulfovibrio strain NY682, oral sulfate-reducing bacteria involved in human periodontal disease.

Langendijk, P. S.; Kulik, Eva M.; Sandmeier, H.; Meyer, J.; Hoeven, J.S. van der

doi:10.1099/00207713-51-3-1035

Cited by 82 publications

(64 citation statements)

References 43 publications

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“…For the tooth pocket material of two patients suffering from adult periodontitis the SRP-PhyloChip hybridization pattern indicated the presence of members of the genus Desulfomicrobium. Colonization of the tooth pockets analyzed by these SRPs, which is consistent with a previous report of isolation of Desulfomicrobium orale from periodontal tooth pockets (33), was independently confirmed by retrieval of 16S rRNA and dsrAB gene sequences of Desulfomicrobium orale, demonstrating the reliability of the microarray results.…”

Section: Discussionsupporting

confidence: 78%

Oligonucleotide Microarray for 16S rRNA Gene-Based Detection of All Recognized Lineages of Sulfate-Reducing Prokaryotes in the Environment

Loy

Lehner

Lee

et al. 2002

Appl Environ Microbiol

604

417

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For cultivation-independent detection of sulfate-reducing prokaryotes (SRPs) an oligonucleotide microarray consisting of 132 16S rRNA gene-targeted oligonucleotide probes (18-mers) having hierarchical and parallel (identical) specificity for the detection of all known lineages of sulfate-reducing prokaryotes (SRP-PhyloChip) was designed and subsequently evaluated with 41 suitable pure cultures of SRPs. The applicability of SRPPhyloChip for diversity screening of SRPs in environmental and clinical samples was tested by using samples from periodontal tooth pockets and from the chemocline of a hypersaline cyanobacterial mat from Solar Lake (Sinai, Egypt). Consistent with previous studies, SRP-PhyloChip indicated the occurrence of Desulfomicrobium spp. in the tooth pockets and the presence of Desulfonema-and Desulfomonile-like SRPs (together with other SRPs) in the chemocline of the mat. The SRP-PhyloChip results were confirmed by several DNA microarrayindependent techniques, including specific PCR amplification, cloning, and sequencing of SRP 16S rRNA genes and the genes encoding the dissimilatory (bi)sulfite reductase (dsrAB).

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Section: Discussionsupporting

confidence: 78%

Oligonucleotide Microarray for 16S rRNA Gene-Based Detection of All Recognized Lineages of Sulfate-Reducing Prokaryotes in the Environment

Loy

Lehner

Lee

et al. 2002

Appl Environ Microbiol

604

417

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“…An increased number of these microorganisms can be found in people with periodontitis, inflammatory bowel diseases, ulcerative colitis, rheumatic diseases and many other diseases [6,8,9,16,18,20,24]. Some scientists also suggest that the sulfatereducing bacteria (SRB) might cause of some forms of colon cancer, taking into account that these microorganisms produce hydrogen sulfide affecting the intestinal cells metabolism causing various diseases [6,15,16,22,24,28].…”

Section: Introductionmentioning

confidence: 99%

Identification of sulfate-reducing bacteria strains of human large intestine

Kushkevych

2013

Biol. Stud.

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The sulfate-reducing bacteria have been isolated from human intestine. These bacteria are identified by their morphological, physiological and biochemical characteristics as the Desulfovibrio sp. and Desulfomicrobium sp. according to Bergey's Manual of Determinative Bacteriology. Growth of the isolated strains of bacteria has been studied under various conditions including types of media, effect of oxygen, pH, and tempe rature. Bacterial growth in the modified KravtsovSorokin's medium, and process of dissimilatory sulfate reduction by the selected strains of bacteria have been investiga ted. All obtained bacterial cultures used sulfate and lactate intensively as well as produced hydrogen sulfide and acetate in the medium. The Desulfovibrio sp. strain Vib7 produced the highest concentration (up to 3.23 mM) of hydrogen sulfide, while the bacteria used about 99% of sulfate ions presented in the medium. This strain also produced the highest concentration of acetate ions (up to 15.87 mM) on the fifth day of cultivation. Among the studied Desulfomicrobium sp. strains, the highest concentration of hydrogen sulfide was produced by the Desulfomicrobium sp. strain Rod9, and the highest concentration of acetate ions was produced by the Desulfomicrobium sp. strain Rod4.

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“…16S rRNA sequencing Distinct sulfate reducing properties Cellular lipid composition profiles (Langendijk et al, 2001) Isolated from periodontal pockets in periodontitis…”

Section: Porphyromonas Uenonis [S]mentioning

confidence: 99%

Exploring the oral bacterial flora: current status and future directions

et al. 2010

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Oral Diseases (2010) 16, 136–145 Objective: The oral cavity forms an indispensable part of the human microbiome, for its unique and diverse microflora distributed within various niches. While majority of these organisms exhibit commensalism, shifts in bacterial community dynamics cause pathological changes within oral cavity and distant sites. The aim of this review was to appraise the current and emerging methods of detecting bacteria of the oral cavity paying particular attention to the cultivation independent methods. Design: Literature pertaining to cultivation based and cultivation independent methods of oral bacterial identification was reviewed. Methods: The specific advantages and disadvantages of cultivation based, microscopic, immunological and metagenomic identification methods were appraised. Results: Because of their fastidious and exacting growth requirements, cultivation based studies grossly underestimate the extent of bacterial diversity in these polymicrobial infections. Culture independent methods deemed more sensitive in identifying difficult to culture and novel bacterial species. Conclusion: Apart from characterizing potentially novel bacterial species, the nucleic acid sequence data analyzed using various bioinformatics protocols have revealed that there are in excess of 700 bacterial species inhabiting the mouth. Moreover, the latest pyrosequencing based methods have further broadened the extent of bacterial diversity in oral niches.

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Isolation of Desulfomicrobium orale sp. nov. and Desulfovibrio strain NY682, oral sulfate-reducing bacteria involved in human periodontal disease.

Cited by 82 publications

References 43 publications

Oligonucleotide Microarray for 16S rRNA Gene-Based Detection of All Recognized Lineages of Sulfate-Reducing Prokaryotes in the Environment

Oligonucleotide Microarray for 16S rRNA Gene-Based Detection of All Recognized Lineages of Sulfate-Reducing Prokaryotes in the Environment

Identification of sulfate-reducing bacteria strains of human large intestine

Exploring the oral bacterial flora: current status and future directions

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