Isolation of cyclic 3',5'-guanosine monophosphate from bacterial culture fluids.

Ishiyama, Jiro

doi:10.1271/bbb1961.39.1331

Cited by 5 publications

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“…UNUSUAL CYCLIC NUCLEOTIDES Cyclic pyrimidines including 3',5'-uridine and -cytidine monophosphates have been found in cultures of Corynebacterium murisepticum and Micrococcus spp. (104). Cyclic deoxyadenosine monophosphate has also been isolated from C. murisepticum (105,107).…”

Section: Cyclic Nucleotides In Dimorphicmentioning

confidence: 99%

Cyclic nucleotides in procaryotes.

Botsford¹

1981

Microbiological Reviews

113

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Section: Cyclic Nucleotides In Dimorphicmentioning

confidence: 99%

Cyclic nucleotides in procaryotes.

Botsford¹

1981

Microbiological Reviews

113

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“…In the case of CRP proteins, cAMP binding results in a large well-understood allosteric transition that allows binding to specific DNA sequences. − The deamination product of cAMP, cIMP, has been reported as a compound released into the growth medium by cultures of Corynebacterium murisepticum and Microbacterium sp . 205 , and a component of mammalian tissue culture cells, although in each of these systems the means of cIMP production are unknown. Deamination of cAMP by a broad specificity fungal adenosine deaminase and in toad bladder extracts has been reported.…”

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Discovery of a cAMP Deaminase That Quenches Cyclic AMP-Dependent Regulation

et al. 2013

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An enzyme of unknown function within the amidohydrolase superfamily was discovered to catalyze the hydrolysis of the universal second messenger, cyclic-3’, 5’-adenosine monophosphate (cAMP). The enzyme, which we have named CadD, is encoded by the human pathogenic bacterium Leptospira interrogans. Although CadD is annotated as an adenosine deaminase, the protein specifically deaminates cAMP to cyclic-3’, 5’-inosine monophosphate (cIMP) with a kcat/Km of 2.7 ± 0.4 × 105 M−1 s−1 and has no activity on adenosine, adenine, or 5’-adenosine monophosphate (AMP). This is the first identification of a deaminase specific for cAMP. Expression of CadD in Escherichia coli mimics the loss of adenylate cyclase in that it blocks growth on carbon sources that require the cAMP-CRP transcriptional activator complex for expression of the cognate genes. The cIMP reaction product cannot replace cAMP as the ligand for CRP binding to DNA in vitro and cIMP is a very poor competitor of cAMP activation of CRP for DNA binding. Transcriptional analyses indicate that CadD expression represses expression of several cAMP-CRP dependent genes. CadD adds a new activity to the cAMP metabolic network and may be a useful tool in intracellular study of cAMP-dependent processes.

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A novel method for the preparation of adenosine 3′5′ cyclic phosphate labelled with ¹⁴C or ³H

Pandey

Nair

Pradhan

1983

Labelled Comp Radiopharmac

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SUMMARYHigh yields of cyclic AMP (CAMP) and cyclic GMP (cGMP) could be obtained by near quantitative conversions of adenine and guanine by aerobically-grown mnp r o l i f e r a t i n g c e l l s of Corynebapterium murisepticum. chemically i n several steps from labelled adenine (2,3). The enzymatic method f o r t h e preparation of labelled CAMP f r o m labelled ATF' is cormoercially unsuitable for large scale preparations since it requires e i t h e r purified ATWsef r e e adenyl cyclase o r crude adenyl cyclase with ATP regenerating system ( 4 ) . The procedure f o r t h e chemicalThe s t a r t i n g material is labelled AMP which i t s e l f is prepared W e have developed a simple, e f f i c i e n t and inexpensive procedure for t h e preparation of labelled CAMP f r o m labelled adenine using nonproliferating c e l l s of Corynebacterium murisepticum.

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Isolation of cyclic 3',5'-guanosine monophosphate from bacterial culture fluids.

Cited by 5 publications

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Cyclic nucleotides in procaryotes.

Cyclic nucleotides in procaryotes.

Discovery of a cAMP Deaminase That Quenches Cyclic AMP-Dependent Regulation

A novel method for the preparation of adenosine 3′5′ cyclic phosphate labelled with ¹⁴C or ³H

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Isolation of cyclic 3',5'-guanosine monophosphate from bacterial culture fluids.

Cited by 5 publications

References 0 publications

Cyclic nucleotides in procaryotes.

Cyclic nucleotides in procaryotes.

Discovery of a cAMP Deaminase That Quenches Cyclic AMP-Dependent Regulation

A novel method for the preparation of adenosine 3′5′ cyclic phosphate labelled with 14C or 3H

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A novel method for the preparation of adenosine 3′5′ cyclic phosphate labelled with ¹⁴C or ³H