Isolation of Cellulolytic Lactic-Acid Bacteria from Mentok (Anas moschata) Gastro-Intestinal Tract

Abstract

Mentok, a local Indonesian name's of muscovy duck (Anas moschata), has been known as a duck with the capability to consume poor quality feed, high with non-starch carbohydrate (NSP) content. This capacity occurs because of the presence of microbial fermentation activity in the gastro intestinal tract (GIT) of mentok. However, the information about the identification and characterization of the cellulolytic microbes involved is limited. This study was expected to provide scientific contributions about gastrointestinal microbes, especially Lactic Acid Bacteria (LAB), with cellulolytic activities. The experiment was conducted to select LAB with cellulolytic activity from the GIT of mentok. Twenty six of selected LABs were isolated from the duodenum, cecum, and colon regions after microbiological characterization, i.e., morphology, catalase test, gas production, Gram staining, and motility test. Characterization for cellulolytic activity was analyzed by measuring the clearing zone on Carboxymethylcellulose (CMC) media, cell growth analysis on 1% CMC as a carbon source, and CMCase value. Pediococcus acidilactici MK 20 isolate from colon region was selected LAB with the highest cellulolytic activity with the clearing zone diameter, and the CMCase value of 2.33 mm and 0.0153 U/mL, respectively. Molecular identification using 16S rRNA gene sequences analysis revealed that P. acidilactici MK 20 isolate has 99% similarity with P. acidilactici strain ZW001. It was concluded that P. acidilactici MK 20 isolated from the colon part of the gastrointestinal tract of mentok, the Indonesian muscovy duck (A. moschata) had cellulolytic activity.

Administration of Fermented Averrhoa bilimbi L. Fruit Filtrate on Growth, Hematological, Intestinal, and Carcass Indices of Broilers

“…Khan and Iqbal (2016) documented that organic acids could increase the activity of fiberdegrading enzymes resulting in increased crude fiber digestibility. Likewise, lactic acid bacteria may produce cellulolytic enzymes that can degrade the crude fiber in the feed as reported by Herdian et al (2018).…”

The Effect of Nanoencapsulated Phaleria macrocarpa Fruits Extract in Drinking Water on Jejunal Histomorphology of Broiler Chickens

“…reported that phenolic compounds of eight berries inhibited the growth of selected gram-negative bacteria and were not active against gram positive LAB. Increasing LAB population in broiler chicken indicated the better gastrointestinal conditions, because LAB potentially improved nutrient digestibility especially with cellulose content (Herdian et al, 2018).…”

Lactic acid production ability of Lactobacillus sp. from four tropical fruits using their by-products as carbon source

“…Just like starch, cellulose can be exploited for LA production if it is in the presence of a LAB able to metabolize it. Saowapar et al (2014) and Herdian et al (2018) in the course of their work showed that the cellulase activity of LAB not only varied between isolates from different biotopes but also varied between isolates from the same biotope. Similar data were observed in our study and cellulase from isolate1B9 showed the most significant activity with a production of 7.98 μg of glucose per ml of solution for one minute.…”

“…Saowapar et al. (2014) and Herdian et al. (2018) in the course of their work showed that the cellulase activity of LAB not only varied between isolates from different biotopes but also varied between isolates from the same biotope.…”

“…The use of lactic acid bacteria (LAB) in the agri-food industry has evolved exponentially over the years as they possess technological properties that can be exploited at the industrial level. These include proteolytic, cellulolytic, flavoring, amylolytic, texturizing, but also their ability to produce lactic acid ( Elmaged et al., 2015 ; Herdian et al., 2018 ; Galli et al., 2020 ). Indeed, lactic acid (LA) is an organic compound that plays a role in multiple biochemical processes; it has various applications cosmetic, pharmaceutical, chemical and food industries ( Mohan et al., 2015 ; Battula et al., 2018 ; Abedi and Hashemi, 2020 ).…”