Isolation of cellulase-producing &lt;i&gt;Microbulbifer&lt;/i&gt; sp. from marine teleost blackfish (&lt;i&gt;Girella melanichthys&lt;/i&gt;) intestine and the enzyme characterization

Tanaka, Daiki; Ohnishi, Ken-Ichiro; Watanabe, Shizuo

doi:10.2323/jgam.2020.05.001

Cited by 10 publications

(4 citation statements)

References 42 publications

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“…A Microbulbifer sp. strain was recently isolated from the intestine of the herbivorous teleost Girella melanichthys and was found to degrade cellulose [ 70 ], a rather useful trait for the omnivorous D. vulgaris midgut in the current study. Thaumasiovibrio is a recently described genus [ 71 ] and has not yet been reported to be associated with fish.…”

Section: Discussionmentioning

confidence: 99%

Midgut Bacterial Microbiota of 12 Fish Species from a Marine Protected Area in the Aegean Sea (Greece)

et al. 2022

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Fish microbiome science is progressing fast, but it is biased toward farmed or laboratory fish species against natural fish populations, which remain considerably underinvestigated. We analyzed the midgut bacterial microbiota of 45 specimens of 12 fish species collected from the Gyaros Island marine protected area (Aegean Sea, Greece). The species belong to seven taxonomic families and are either herbivores or omnivores. Mucosa midgut bacterial diversity was assessed by amplicon metabarcoding of the 16S rRNA V3–V4 gene region. A total of 854 operational taxonomic units (OTUs) were identified. In each fish species, between 2 and 18 OTUs dominated with cumulative relative abundance ≥ 70%. Most of the dominating bacterial taxa have been reported to occur both in wild and farmed fish populations. The midgut bacterial communities were different among the 12 fish species, except for Pagrus pagrus and Pagellus erythrinus, which belong to the Sparidae family. No differentiation of the midgut bacterial microbiota was found based on feeding habits, i.e., omnivorous vs. carnivorous. Comparing wild and farmed P. pagrus midgut bacterial microbiota revealed considerable variation between them. Our results expand the gut microbiota of wild fish and support the host species effect as the more likely factor shaping intestinal bacterial microbiota.

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Section: Discussionmentioning

confidence: 99%

Midgut Bacterial Microbiota of 12 Fish Species from a Marine Protected Area in the Aegean Sea (Greece)

et al. 2022

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“…A Microbulbifer sp. strain was recently isolated from the intestine of the herbivorous teleost Girella melanichthys and was found to degrade cellulose [Tanaka et al 2021], a rather useful trait for the omnivorous D. vulgaris midgut in the current study. Thaumasiovibrio is a recently described genus [Amin et al 2017] and has not yet been reported to be associated with fish.…”

Section: Discussionmentioning

confidence: 99%

Midgut bacterial microbiota of 12 fish species from a marine protected area in the Aegean Sea (Greece)

Kormas

Nikouli

Kousteni

et al. 2022

Preprint

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Fish microbiome science is progressing fast, but it is biased toward farmed or labora-tory fish species against natural fish populations, which remain considerably underinvestigated. We analysed the midgut bacterial microbiota of 45 specimens of 12 fish species collected from the Gyaros Island marine protected area (Aegean Sea, Greece). The species belong to seven taxonomic families and are either herbivores or omnivores. Mucosa midgut bacterial diversity was assessed by amplicon metabarcoding of the 16S rRNA V3–V4 gene region. A total of 854 operational tax-onomic units were identified, of which 28 were shared among all samples. The majority of the dominating bacterial taxa have been reported to occur both in wild and farmed fish populations. The midgut bacterial communities were different among the 12 fish, except for Pagrus pagrus and Pagellus erythrinus, which belong to the Sparidae family. No differentiation of the midgut mi-crobiota was found based on feeding habits: omnivorous vs. carnivorous. Comparing wild and farmed P. pagrus midgut microbiota revealed considerable variation between them. Our results expand the gut microbiota of wild fish and also support the host phylogeny as the prime factor shaping intestinal microbiota.

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“…Microbulbifer sp. Strain GL-2, the bacterium used in the present study, was isolated from the intestine of a blackfish (Girella melanichthys) (Tanaka et al, 2021). The whole-genome sequence of this strain has been deposited in DDBJ/EMBL/GenBank as Accession Number AP019807 (Sugimoto et al, 2020); a circular map of the genome is shown in Fig.…”

Section: Bacterial Strains and Culturementioning

confidence: 99%

“…In previous work, we isolated Microbulbifer sp. Strain GL-2 from the intestine of blackfish (Girella melanichthys), an omnivorous marine teleost, and reported that this bacterium exhibits high levels of cellulase activity (Tanaka et al, 2021). Species of the genus Microbulbifer, proposed by González et al (1997), have been isolated from marine sediments (Zhang et al, 2011), deep-sea sediments (Miyazaki et al, 2008), and seaweeds (Nishijima et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Cellulolytic enzymes in <i>Microbulbifer</i> sp. Strain GL-2, a marine fish intestinal bacterium, with emphasis on endo-1,4-β-glucanases Cel5A and Cel8

Ohnishi,

Watanabe,

Kadoya

et al. 2024

J. Gen. Appl. Microbiol.

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Isolation of cellulase-producing <i>Microbulbifer</i> sp. from marine teleost blackfish (<i>Girella melanichthys</i>) intestine and the enzyme characterization

Abstract: Isolation of cellulase-producing Microbulbifer sp. from marine teleost blackfish (Girella melanichthys) intestine and the enzyme characterization

Cited by 10 publications

References 42 publications

Midgut Bacterial Microbiota of 12 Fish Species from a Marine Protected Area in the Aegean Sea (Greece)

Midgut Bacterial Microbiota of 12 Fish Species from a Marine Protected Area in the Aegean Sea (Greece)

Midgut bacterial microbiota of 12 fish species from a marine protected area in the Aegean Sea (Greece)

Cellulolytic enzymes in <i>Microbulbifer</i> sp. Strain GL-2, a marine fish intestinal bacterium, with emphasis on endo-1,4-β-glucanases Cel5A and Cel8

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